Microbial agent as well as preparation method and application

Abstract

The invention relates to a microbial agent as well as a preparation method and application thereof, and belongs to the field of comprehensive prevention and control of plant diseases. The microbial agent is prepared from functional microorganism, a nutritional agent and a protective agent, wherein the microorganism is prepared from streptomyces jingyangensis, streptomyces lavendulae, streptomycesaureofaciens and viridin. The functional microorganism of the microbial agent is screened from the natural world; the effect is achieved in a way of inhibiting bacteria by the bacteria, pathogenic bacteria is killed, environmental pollution is avoided and the using of medicines is reduced; the microbial agent is safe and harmless, a harmful influence to crops and a human body is avoided, and the microbial agent can be applied to spraying of plants, soaking of seeds, spray coating of seedlings, disinfection of irrigation water, disinfection of farm tools, soil treatment and the like; a route oftransmission of the pathogenic bacteria can be blocked in many ways so as to prevent and control the diseases; a using method of the microbial agent is flexible, safe and easy.

Description

technical field

[0001] The invention relates to a microbial agent, a preparation method and application thereof, and belongs to the field of comprehensive prevention and control of plant diseases. Background technique

[0002] Tomato canker is one of the most serious diseases in tomato production. Since it was first reported in the United States, it has spread rapidly and spread to many tomato-producing areas in the world, gradually becoming a worldwide disease. At present, the disease is distributed in North America, Europe, Asia, Africa, and Oceania, covering more than 60 countries such as the United States, the United Kingdom, Canada, Germany, and Japan. Tomato canker was first discovered in Pinggu, Beijing in 1985 in my country, and has now spread to many tomato producing areas. In 1993, the occurrence of tomato canker in Northeast my country was confirmed for the first time. In 2002, the incidence rate of tomato in Fangtang Township, Guoning City, Anhui Province exceed...

Examples

Embodiment 1

[0020] Screening of functional microorganisms

[0021] In tomato canker disease-infected areas, soil samples were collected from areas with lesser disease levels, as well as leaf samples of diseased plants without disease, and microorganisms were isolated, cultivated and preserved. After preliminary screening, 7 strains of bacteria were obtained from soil samples. Numbered A01-A07, 4 strains were isolated from leaf samples, numbered A08-A11. At the same time, Bacillus subtilis, Bacillus sporogenes, Bacillus jelly-like, Trichoderma harzianum, Verticillium, Streptomyces Jingyang, Paecilomyces lilacinus, and yellow were selected from the laboratory strain bank for plant disease control. Trichoderma, powdery mildew parasite 9 strains. The antibacterial test was carried out on 11 strains of primary screening strains and 9 strains of laboratory strains to observe the inhibitory effect of each strain on the pathogenic bacteria of tomato canker. The specific results are as follows: ...

Embodiment 2

[0027] preparation of microbial agents

[0028] A: Mix Streptomyces jingyang, Streptomyces lavender, Streptomyces aureus, and Trichoderma viride in a ratio of 1:1:1:1 to make microbial powder.

[0029] B: Glucose, whey powder, and magnesium sulfate are mixed in a ratio of 60:35:5 to prepare a nutritional supplement.

[0030] C: Mix trehalose, ascorbic acid, and copper sulfate in a ratio of 50:40:10 to prepare a protective agent.

[0031] D: The microbial bacterial powder, nutrient, and protective agent were mixed evenly, and vacuum-packed to prepare a microbial bacterial agent. The measured viable bacteria amount in the bacterial agent was 2.4 billion / g.

Embodiment 3

[0033] Investigate the antibacterial effect and dosage of microbial agents through experiments

[0034] Tomato seedlings were planted in a pot experiment. After 5 days, the seedlings with the same growth were selected, and the pathogenic bacteria were inoculated by artificial inoculation. After the symptoms of the disease appeared, they were placed in different isolated areas for later use. The microbial bacterial agent prepared in Example 1 was diluted 500 times, 1000 times, 1500 times, 2000 times, 2500 times and 3000 times respectively to prepare bacterial liquids of different concentrations, and sprayed the diseased seedlings according to the normal spraying speed. Then manage according to the conventional method, spray once every 7 days, and observe the disease of the plants.

[0035] Through experiments, it was found that in the groups diluted 2500 times and 3000 times, the spread of symptoms slowed down significantly, but it was still spreading slowly, indicating that th...

technical field

[0001] The invention relates to a microbial agent, a preparation method and application thereof, and belongs to the field of comprehensive prevention and control of plant diseases. Background technique

[0002] Tomato canker is one of the most serious diseases in tomato production. Since it was first reported in the United States, it has spread rapidly and spread to many tomato-producing areas in the world, gradually becoming a worldwide disease. At present, the disease is distributed in North America, Europe, Asia, Africa, and Oceania, covering more than 60 countries such as the United States, the United Kingdom, Canada, Germany, and Japan. Tomato canker was first discovered in Pinggu, Beijing in 1985 in my country, and has now spread to many tomato producing areas. In 1993, the occurrence of tomato canker in Northeast my country was confirmed for the first time. In 2002, the incidence rate of tomato in Fangtang Township, Guoning City, Anhui Province exceed...

Examples

Embodiment 1

[0020] Screening of functional microorganisms

[0021] In tomato canker disease-infected areas, soil samples were collected from areas with lesser disease levels, as well as leaf samples of diseased plants without disease, and microorganisms were isolated, cultivated and preserved. After preliminary screening, 7 strains of bacteria were obtained from soil samples. Numbered A01-A07, 4 strains were isolated from leaf samples, numbered A08-A11. At the same time, Bacillus subtilis, Bacillus sporogenes, Bacillus jelly-like, Trichoderma harzianum, Verticillium, Streptomyces Jingyang, Paecilomyces lilacinus, and yellow were selected from the laboratory strain bank for plant disease control. Trichoderma, powdery mildew parasite 9 strains. The antibacterial test was carried out on 11 strains of primary screening strains and 9 strains of laboratory strains to observe the inhibitory effect of each strain on the pathogenic bacteria of tomato canker. The specific results are as follows: ...

Embodiment 2

[0027] preparation of microbial agents

[0028] A: Mix Streptomyces jingyang, Streptomyces lavender, Streptomyces aureus, and Trichoderma viride in a ratio of 1:1:1:1 to make microbial powder.

[0029] B: Glucose, whey powder, and magnesium sulfate are mixed in a ratio of 60:35:5 to prepare a nutritional supplement.

[0030] C: Mix trehalose, ascorbic acid, and copper sulfate in a ratio of 50:40:10 to prepare a protective agent.

[0031] D: The microbial bacterial powder, nutrient, and protective agent were mixed evenly, and vacuum-packed to prepare a microbial bacterial agent. The measured viable bacteria amount in the bacterial agent was 2.4 billion / g.

Embodiment 3

[0033] Investigate the antibacterial effect and dosage of microbial agents through experiments

[0034] Tomato seedlings were planted in a pot experiment. After 5 days, the seedlings with the same growth were selected, and the pathogenic bacteria were inoculated by artificial inoculation. After the symptoms of the disease appeared, they were placed in different isolated areas for later use. The microbial bacterial agent prepared in Example 1 was diluted 500 times, 1000 times, 1500 times, 2000 times, 2500 times and 3000 times respectively to prepare bacterial liquids of different concentrations, and sprayed the diseased seedlings according to the normal spraying speed. Then manage according to the conventional method, spray once every 7 days, and observe the disease of the plants.

[0035] Through experiments, it was found that in the groups diluted 2500 times and 3000 times, the spread of symptoms slowed down significantly, but it was still spreading slowly, indicating that th...

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