Detection reagent for triglyceride and detection test paper for triglyceride

A technology for detecting reagents and triglycerides, applied in the field of detection, can solve the problems of high blood consumption, cumbersome production process, uneven blood samples in the reaction layer, etc., and achieve the effect of avoiding outflow and removing interference

Inactive Publication Date: 2018-05-04
民康医疗科技(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is that the upper shell has only one sample injection hole (either a circular hole or a rectangular hole), and the blood must diffuse to the left and right sides so that the detection holes on both sides can also have blood for testing
[0010] The second deficiency is that there is only one sample injection hole (either a circular hole or a rectangular hole) on the upper shell. When the blood diffuses to the left and right sides, there is a possibility of uneven blood samples in the reaction layer on the left and right sides.
However, the blood consumption of this structure is still relatively high, and its production process is relatively cumbersome

Method used

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  • Detection reagent for triglyceride and detection test paper for triglyceride
  • Detection reagent for triglyceride and detection test paper for triglyceride
  • Detection reagent for triglyceride and detection test paper for triglyceride

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preparation example Construction

[0051] The preparation method of the detection test paper of described triglyceride, comprises the steps:

[0052] (1) spraying the detection reagent onto the reaction layer material, and then drying at a drying temperature of 35-50° C. and a drying time of 20-45 minutes to obtain a reaction layer material containing the reaction reagent;

[0053] (2) Cutting the materials of the diffusion layer, the filter layer, the hydrophilic layer and the reaction layer into required sizes respectively to obtain the diffusion layer, the filter layer, the hydrophilic layer and the reaction layer;

[0054] (3) stacking an upper reaction layer, a hydrophilic layer, a filter layer, and a diffusion layer sequentially from bottom to top on the lower shell;

[0055] (4) Cover the upper shell on the diffusion layer, and then cut it to obtain the test paper for detecting triglyceride.

Embodiment 1

[0057] A detection reagent for triglycerides, the detection reagent comprises 400KU / L of lipoprotein lipase, 45KU / L of glycerol kinase, 30KU / L of glycerol triphosphate oxidase, 10KU / L of horseradish peroxidase, 1mmol / L 4-aminoantipyrine, 1mmol / L chromogen, 5mmol / L Mg 2+ , 0.2% BSA, 1% Trition-100.

[0058] A triglyceride detection test paper comprises a diffusion layer, a filter layer, a hydrophilic layer and a reaction layer which are sequentially arranged from top to bottom, and the surface of the reaction layer is coated with the detection reagent.

[0059] The material of the diffusion layer is PES, the material of the filter layer is PES, the material of the hydrophilic layer is PES, and the material of the reaction layer is PES.

[0060] The thickness of the diffusion layer is 242 μm; the thickness of the filter layer is 353 μm; the thickness of the hydrophilic layer is 268 μm; the thickness of the reaction layer is 114 μm.

[0061] The pore size of the material of the...

Embodiment 2

[0070] A detection reagent for triglycerides, the detection reagent comprises 400KU / L lipoprotein lipase, 45KU / L glycerol kinase, 37.5KU / L glycerol triphosphate oxidase, 15KU / L horseradish peroxidase , 1mmol / L of 4-aminoantipyrine, 1mmol / L of chromogen, 5mmol / L of Mg 2+ , 1% BSA, 1% Trition-100.

[0071] A triglyceride detection test paper comprises a diffusion layer, a filter layer, a hydrophilic layer and a reaction layer which are sequentially arranged from top to bottom, and the surface of the reaction layer is coated with the detection reagent.

[0072] The material of the diffusion layer is PES, the material of the filter layer is PES, the material of the hydrophilic layer is PES, and the material of the reaction layer is PES.

[0073] The thickness of the diffusion layer is 242 μm; the thickness of the filter layer is 353 μm; the thickness of the hydrophilic layer is 268 μm; the thickness of the reaction layer is 114 μm.

[0074] The pore size of the material of the d...

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Abstract

The invention provides a detection reagent for triglyceride and detection test paper for triglyceride. The detection reagent comprises 80-400KU / L lipoprotein lipase, 9-65KU / L glycerol kinase, 6-45KU / Lglycerol triphosphate oxidase, 2-25KU / L horse radish peroxidase, 0.8-1.2mmol / L 4-aminoantipyrine, 0.6-1mmol / L chromogen and 1.0-5mmol / L Mg<2+>. The detection reagent for triglyceride can remove interference and can measure the level of triglyceride quickly and accurately.

Description

technical field [0001] The invention belongs to the detection field, in particular to a triglyceride detection reagent and a triglyceride detection test paper. Background technique [0002] The analysis of samples can generally be divided into two categories of techniques: dry chemical analysis techniques and wet chemical analysis techniques. Dry chemical analysis technology is relative to wet chemical technology. It refers to adding liquid detection samples directly to dry test paper (dry test paper), and using the moisture of the tested sample as a solvent to cause a specific chemical reaction, thereby performing chemical analysis. method of analysis. [0003] According to the flow path of the liquid sample on the dry chemical test paper, it is divided into a lateral-flow method (lateral-flow) or a vertical-flow method (vertical-flow). [0004] The structure of the dry test strip using the lateral flow method generally includes a hard impermeable bottom plate, on which t...

Claims

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Application Information

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IPC IPC(8): G01N33/52
CPCG01N33/526
Inventor 周敬丽甘建民
Owner 民康医疗科技(天津)有限公司
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