Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cryopreserving liquid for cryopreserving single karyocyte

A nuclear cell and cryopreservation solution technology, applied in the field of low-temperature cryopreservation of biotechnology, can solve the problems of treatment impact, increase the possibility of contamination by animal pathogens, etc., reduce the amount of introduction, reduce the possibility of animal pathogen contamination, and differentiate powerful effect

Active Publication Date: 2018-05-15
山东省齐鲁细胞治疗工程技术有限公司 +1
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fetal bovine serum has both advantages and disadvantages. Its biggest disadvantage is the introduction of animal protein, which increases the possibility of contamination by animal pathogens and will have a certain impact on human adoptive immune cell therapy.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cryopreserving liquid for cryopreserving single karyocyte
  • Cryopreserving liquid for cryopreserving single karyocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: preparation dextran solution reagent

[0028] Use an analytical balance to weigh 2g of dextran with a molecular weight of 40,000, put it in a 50ml centrifuge tube, pipette 20ml of 1×PBS into the above centrifuge tube, mix well and put it in a 4°C refrigerator for later use.

Embodiment 2

[0029] Embodiment 2: Preparation of mononuclear cell cryopreservation solution No. 6 (fetal bovine serum, dimethyl sulfoxide)

[0030] Take 90% fetal bovine serum by volume percentage, then slowly add 10% dimethyl sulfoxide dropwise, and finally shake well to prepare mononuclear cell cryopreservation solution. Store at 4°C for later use.

Embodiment 3

[0031] Embodiment 3: Preparation of mononuclear cell cryopreservation liquid No. 9 (fetal bovine serum, dimethyl sulfoxide, cell culture medium)

[0032] Take 40% fetal bovine serum by volume percentage, then slowly add 10% dimethyl sulfoxide dropwise, and finally add 50% cell culture medium and shake well to prepare a mononuclear cell cryopreservation solution. Store at 4°C for later use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses cryopreserving liquid for cryopreserving a single karyocyte. The cryopreserving liquid is prepared from fetal bovine serum, dimethyl sulfoxide and dextran dissolving liquid, wherein the fetal bovine serum accounts for 45 to 95 percent; the dextran dissolving liquid accounts for 10 to 50 percent; the dimethyl sulfoxide accounts for 5 to 10 percent. Dextran refers to low-molecular-weight dextran; a molecular weight of the dextran is 40,000. The cryopreserving liquid uses the low-molecular-weight dextran to replace part of animal serum, and can be used for effectively reducing an introduction amount of foreign protein. The cryopreserving liquid provided by the invention can be used for effectively retaining the cell activity of the single karyocyte which is directly cryopreserved after being extracted. If the single karyocyte is subjected to recovery culture at an interval after being subjected to cryopreservation treatment by using the cryopreserving liquid provided by the invention, a cell recovery rate can be maintained at a higher level, and further, the cell differentiation capacity is still high.

Description

technical field [0001] The invention relates to a cryopreservation solution for cryopreservation of mononuclear cells, which belongs to the field of low-temperature cryopreservation of biotechnology. Background technique [0002] The immune activity of mononuclear cells in the human body is closely related to the age and health of the individual. How can mononuclear cells be preserved for a longer period of time is a problem. If the cells are frozen, whether they can maintain their original activity after recovery is also a challenge. [0003] Cryopreservation of mononuclear cells can maintain their viability and function, which is of great significance for the induction culture of later cells and the direct treatment of patients after recovery of cryopreserved cells. Cryopreservation of mononuclear cells can not only solve the problem of repeated induction of cells during multiple blood collection and culture of patients, but also preserve the mononuclear cells with the st...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 谭毅郭士朋郭慧贞吴冰梅
Owner 山东省齐鲁细胞治疗工程技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com