A method for promoting yeast cell wall breaking

Abstract

The invention discloses a method for promoting yeast cell wall breaking. The yeast milk is sprayed at high speed by an injector, heated by steam, and then passed through a buffer container with a large volume to fully disperse the yeast milk, and the temperature is rapidly raised and then passed through isentropic Reduce pressure and expand to achieve rapid wall breaking of yeast cells. This method can achieve yeast cell wall breaking within 2 hours, and can quickly increase the temperature of yeast emulsion to the temperature required by the post-processing process; after enzymatic treatment in the subsequent section, this method The enzymatic hydrolysis effect basically equal to that of other wall-breaking methods can be achieved, the production efficiency is improved, and the production cost is reduced.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for promoting yeast cell wall breaking. Background technique

[0002] Yeast is a single-cell eukaryotic microorganism, rich in protein, nucleic acid, functional polysaccharides, vitamins and various mineral elements, and is widely used in the fields of food production and feed production. When extracting and producing functional substances such as yeast nucleic acid from yeast cells, producing yeast extracts commonly used in the food industry, yeast autolyzed powder, etc., and producing yeast hydrolyzates commonly used in the feed industry, it is first necessary to break the yeast cells .

[0003] The currently commonly used yeast wall breaking methods include: physical methods, chemical methods, and methods combining physical and chemical methods; physical methods include repeated freezing and thawing methods, ultrasonic methods, extrusion wall breaking methods, high-press...

Examples

Embodiment 1

[0043] The method for promoting yeast cell wall breaking includes:

[0044] Prepare yeast milk with a concentration of 10%, open the steam valve of the injector, introduce jet steam, and turn on the feed pump to pump the yeast milk into the injector, adjust the opening of the injector nozzle needle valve, so that the injection of yeast milk Flow control is 3m 3 / h, to maintain an appropriate film thickness, so that the yeast milk can fully contact and exchange heat with the steam; set the injection temperature of the yeast milk to 70°C, adjust the steam flow through the automatic control system, and maintain the temperature of the yeast milk to keep stable, so that Yeast milk reaches the set temperature and is continuously and stably sprayed into the 0.5m 3 in the maintenance tank, and then flow into the 1.5 m 3 In the flash tank, keep warm for 40 minutes, flow out of the flash tank, and enter the post-treatment (enzymatic hydrolysis) process.

Embodiment 2

[0046] The method for promoting yeast cell wall breaking includes:

[0047] Prepare yeast milk with a concentration of 12%, open the steam valve of the injector, introduce jet steam, and turn on the feed pump to pump the yeast milk into the injector, adjust the opening of the injector nozzle needle valve, so that the injection of yeast milk Flow control is 12m 3 / h, to maintain an appropriate film thickness, so that the yeast milk can fully contact and exchange heat with the steam; set the injection temperature of the yeast milk to 90°C, adjust the steam flow through the automatic control system, and maintain the temperature of the yeast milk to keep stable, so that Yeast milk reaches the set temperature and is continuously and stably sprayed into the 0.5m 3 in the maintenance tank, and then flow into the 1.5 m 3 In the flash tank, keep warm for 10 minutes, flow out of the flash tank, and enter the post-treatment (enzymatic hydrolysis) process.

Embodiment 3

[0049] The method for promoting yeast cell wall breaking includes:

[0050] Prepare yeast milk with a concentration of 3%, open the steam valve of the injector, introduce jet steam, and turn on the feed pump to pump the yeast milk into the injector, adjust the opening of the needle valve of the injector nozzle, so that the injection of yeast milk Flow control is 1m 3 / h, to maintain an appropriate film thickness so that the yeast milk can fully contact and exchange heat with the steam; set the injection temperature of the yeast milk to 50°C, adjust the steam flow through the automatic control system, and maintain the temperature of the yeast milk to keep stable, so that Yeast milk reaches the set temperature and is continuously and stably sprayed into the 0.5m 3 in the maintenance tank, and then flow into the 1.5 m 3 In the flash tank, keep warm for 25 minutes, flow out of the flash tank, and enter the post-treatment (enzymatic hydrolysis) process.

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for promoting yeast cell wall breaking. Background technique

[0002] Yeast is a single-cell eukaryotic microorganism, rich in protein, nucleic acid, functional polysaccharides, vitamins and various mineral elements, and is widely used in the fields of food production and feed production. When extracting and producing functional substances such as yeast nucleic acid from yeast cells, producing yeast extracts commonly used in the food industry, yeast autolyzed powder, etc., and producing yeast hydrolyzates commonly used in the feed industry, it is first necessary to break the yeast cells .

[0003] The currently commonly used yeast wall breaking methods include: physical methods, chemical methods, and methods combining physical and chemical methods; physical methods include repeated freezing and thawing methods, ultrasonic methods, extrusion wall breaking methods, high-press...

Examples

Embodiment 1

[0043] The method for promoting yeast cell wall breaking includes:

[0044] Prepare yeast milk with a concentration of 10%, open the steam valve of the injector, introduce jet steam, and turn on the feed pump to pump the yeast milk into the injector, adjust the opening of the injector nozzle needle valve, so that the injection of yeast milk Flow control is 3m 3 / h, to maintain an appropriate film thickness, so that the yeast milk can fully contact and exchange heat with the steam; set the injection temperature of the yeast milk to 70°C, adjust the steam flow through the automatic control system, and maintain the temperature of the yeast milk to keep stable, so that Yeast milk reaches the set temperature and is continuously and stably sprayed into the 0.5m 3 in the maintenance tank, and then flow into the 1.5 m 3 In the flash tank, keep warm for 40 minutes, flow out of the flash tank, and enter the post-treatment (enzymatic hydrolysis) process.

Embodiment 2

[0046] The method for promoting yeast cell wall breaking includes:

[0047] Prepare yeast milk with a concentration of 12%, open the steam valve of the injector, introduce jet steam, and turn on the feed pump to pump the yeast milk into the injector, adjust the opening of the injector nozzle needle valve, so that the injection of yeast milk Flow control is 12m 3 / h, to maintain an appropriate film thickness, so that the yeast milk can fully contact and exchange heat with the steam; set the injection temperature of the yeast milk to 90°C, adjust the steam flow through the automatic control system, and maintain the temperature of the yeast milk to keep stable, so that Yeast milk reaches the set temperature and is continuously and stably sprayed into the 0.5m 3 in the maintenance tank, and then flow into the 1.5 m 3 In the flash tank, keep warm for 10 minutes, flow out of the flash tank, and enter the post-treatment (enzymatic hydrolysis) process.

Embodiment 3

[0049] The method for promoting yeast cell wall breaking includes:

[0050] Prepare yeast milk with a concentration of 3%, open the steam valve of the injector, introduce jet steam, and turn on the feed pump to pump the yeast milk into the injector, adjust the opening of the needle valve of the injector nozzle, so that the injection of yeast milk Flow control is 1m 3 / h, to maintain an appropriate film thickness so that the yeast milk can fully contact and exchange heat with the steam; set the injection temperature of the yeast milk to 50°C, adjust the steam flow through the automatic control system, and maintain the temperature of the yeast milk to keep stable, so that Yeast milk reaches the set temperature and is continuously and stably sprayed into the 0.5m 3 in the maintenance tank, and then flow into the 1.5 m 3 In the flash tank, keep warm for 25 minutes, flow out of the flash tank, and enter the post-treatment (enzymatic hydrolysis) process.

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