Primer group for detecting genotype in pharmacogenomics, kit and detection method for instructing personalized medicine administration

A detection kit and genomics technology, applied in biochemical equipment and methods, recombinant DNA technology, and microbial assay/inspection, etc., can solve the problems of long cycle, high cost, unable to meet clinical needs, etc. The method is simple and easy to implement, and the effect that meets the clinical needs

Active Publication Date: 2018-05-18
神州医学数据科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you want to detect multiple sites, you can only perform multiple test tests, which is not only costly, but also has a long cycle, which cannot meet clinical needs

Method used

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  • Primer group for detecting genotype in pharmacogenomics, kit and detection method for instructing personalized medicine administration
  • Primer group for detecting genotype in pharmacogenomics, kit and detection method for instructing personalized medicine administration
  • Primer group for detecting genotype in pharmacogenomics, kit and detection method for instructing personalized medicine administration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Query information

[0039] 1. Major guidelines: CPIC, DPWG, FDA, CFDA and other relevant authoritative platforms give information about the relationship between germline variant and drug metabolism and drug response. The information comes from the official websites of major platforms.

[0040] 2. Pubmed related information: 1) search for the main keyword pharmacogenetics; 2) search for information from the perspective of variant affecting final drug metabolism and drug response, including metabolic enzymes of related drugs, drug transporters, drug targets and regulation of drug pathways factor.

[0041] 3. The above various information collection methods, with the help of "Machine Learning-Based Biomedical Text Classification Method, System and Storage Medium" (PN1750734IBJ) help the entire manual search and proofreading process, and finally manual correction review.

[0042] 2. Evaluate query data:

[0043] 1. Classify according to the experimental design plan of...

Embodiment 2

[0055] A detection method for guiding individualized medication, the steps are as follows:

[0056] 1) Extract gDNA, the sample is the subject's venous blood or oral epithelial cells, using the DNA extraction kit Qiagen FlexiGene DNA Kit (250) (Cat No.51206) from Qiagen Company, and extracting according to the instructions.

[0057] (2) Building a database

[0058] Step 1 The first round of multiplex PCR reaction

[0059] 1. Multiplex PCR reaction system

[0060] The first round of multiplex PCR reaction was divided into 2 reaction tubes, and the multiplex primers used were Primer pool T1 (SEQ ID NO: 1-195) and Primer pool T2 (SEQ ID NO: 196-390). The other reagents in the 2 reaction tubes are the same.

[0061] The specific reaction system is shown in Table 1.

[0062] Table 1 reaction system

[0063] wxya 2 o

10-x

5×Multiple buffers

5

Enhancer NB(1N)

5

dNTPs (10mM)

0.5

Primer pool

4

gDNA

x

Multiple e...

Embodiment 3

[0112] According to the method of Example 2, the sample is detected, the detection equipment: Illumina-2500, the number of detection genes is 77, and the effective types of detection drugs are 62. The results of other detection drugs are meaningless and will not be listed. The results are shown in Table 4 .

[0113] Table 4 Test results

[0114]

[0115]

[0116] In the table, others indicate: asparaginase, cytarabine, daunorubicin, dexamethasone, etoposide, calcium folinate, methotrexate, prednisone (glucocorticoid drugs), Changchun Neobase.

[0117] In the table, √ means taking the medicine according to the instructions or according to the doctor's advice;! Indicates that there is a certain risk of toxic and side effects or that the efficacy of the drug is poor.

[0118] The detection method for guiding individualized medication provided by the present invention has been verified by a large number of experiments, and all of them can accurately detect the target gene...

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Abstract

The invention relates to the field of precise medicines, in particular to a primer group for detecting genotype in pharmacogenomics, a kit and a detection method for instructing personalized medicineadministration. The primer group for detecting the genotype in the pharmacogenomics comprises 390 primers, wherein nucleic acid sequences of the 390 primers are shown in SEQ ID NO:1 to 390. The primergroup for detecting the genotype in the pharmacogenomics has the advantages that after massive screening and analyzing, the primer is designed for 77 genes and 219 sites, so as to obtain the primer group; the primer group can simultaneously detect the safety, validity and toxic or side effects of 160 types of medicines in different individuals, so that the cost is reduced, and the detection efficiency is improved; demonstrated by test for multiple times, the target gene can be accurately detected, the condition of reaction to the medicine can be stably and accurately reflected, and the clinical requirement can be well met.

Description

technical field [0001] The invention relates to the field of gene detection for individualized medicine, in particular to a primer set for detecting pharmacogenomics genotype, a kit and a detection method for guiding individualized medicine. Background technique [0002] Pharmacogenomics (Pharmacogenomics, PGx) is a discipline that studies the different responses of different diseases to drugs caused by gene variation. Its main research content is the impact of genome or gene variation on drug absorption, metabolism, efficacy and adverse reactions in the human body. phenomenon and its mechanism. In July 2016, the Clinical Pharmacology Implementation Consortium (CPIC) published a document in Geneticsin Medicine, dividing pharmacogenomics-related genes into three categories: drug-metabolizing enzymes (CYP family, UGT1A1, DPYD and TPMT), drug transporters (such as SLCO1B1), high-risk genotypes (such as HLA-B). [0003] Genetic variation of drug metabolism, transport and drug ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6883C12Q2600/106C12Q2600/16C12Q2531/113C12Q2537/143
Inventor 不公告发明人
Owner 神州医学数据科技(北京)有限公司
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