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Application of kb-r7943 or bepridil in the preparation of medicine for treating glioma

A KB-R7943, glioma technology, applied in the field of biomedicine, to achieve the effect of quick effect, good curative effect and reducing social burden

Active Publication Date: 2019-10-11
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report about the application of compound KB-R7943 or Bepridil in the preparation of drugs for the treatment of glioma

Method used

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  • Application of kb-r7943 or bepridil in the preparation of medicine for treating glioma
  • Application of kb-r7943 or bepridil in the preparation of medicine for treating glioma
  • Application of kb-r7943 or bepridil in the preparation of medicine for treating glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Cell experiment

[0037]During the experiment, human glioma cell lines U87, U251 and SF188 were cultured with DMEM+10% fetal bovine serum, and the cells were seeded in 96-well plates at 8000 cells / well, and placed in a 37°C incubator containing 5% carbon dioxide Internal culture. After the cells grow to 70%-80%, add a series of different concentrations of drugs (KB-R7943 or Bepridil) to the medium, and observe the survival and death of glioma cells after 3 days of drug incubation. Methods to detect cell viability. It was found that both KB-R7943 and Bepridil could significantly inhibit the growth of tumor cells, and their 50% inhibitory concentration (IC 50 ) are 17.30 μM and 12.93 μM respectively, and can kill tumor cells when the concentration is greater than 20 μM. The dose-effect relationship of KB-R7943 and Bepridil in inhibiting the growth of glioma cells figure 2 shown.

[0038] Temozolomide (TMZ) is currently the first-line chemotherapy drug for ...

Embodiment 2

[0041] Embodiment 2 in vivo animal experiments

[0042] 1) Culture and in vivo planting of rat C6 glioma cells:

[0043] Rat C6 glioma cells were cultured in high-glucose DMEM containing 10% fetal bovine serum at 37°C with 5% CO 2 subcultured in an incubator. Take the cells in the logarithmic growth phase, wash with PBS and digest with 0.25% trypsin, collect the cells with PBS to make a cell suspension, the trypan blue staining test identifies that the cell survival rate is greater than 92%, adjust the cell concentration to 100000 / μl and then plant to In the rat brain.

[0044] The implantation of the orthotopic glioma model in rats, C6 cells were implanted into the caudate nucleus of the right brain of the rats. Forty-five clean-grade Wistar rats, 200-310 g each, were used, all male, purchased from the Experimental Animal Center of Shanghai Jiao Tong University School of Medicine. Rats were anesthetized by intraperitoneal injection of 2% pentobarbital sodium, and fixed on...

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Abstract

The invention discloses application of KB-R7943 or Bepridil in preparing a medicine for treating gliomas. The invention discloses a novel strategy in treating gliomas, that is, gliomas are treated byusing a sodium-calcium permutoid inhibitor KB-R7943 or Bepridil, and an old medicine has new application. KB-R7943 and Bepridil have tumor cell killing mechanisms which are completely different from those of conventional medicines, and have no drug resistance as well; in addition, both KB-R7943 and Bepridil are capable of improving sensitivity of gliomas to other common chemotherapeutic drugs; dueto adoption of the two novel medicines, prognosis of patients suffering from gliomas can be improved, the survival rate of the patients can be increased, the social burden can be alleviated, and goodprospects can be achieved for treatment on gliomas.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of sodium-calcium exchanger inhibitor KB-R7943 or Bepridil in the preparation of drugs for treating neuroglioma. Background technique [0002] Glioma is the most common malignant tumor of the human brain, accounting for 40% to 50% of central nervous system tumors, mainly divided into astrocytoma, glioblastoma, oligodendroglioma, and medulloblastoma , Ependymoma and other types. The English name of glioma is glioma or glioblastoma. Glioma patients are divided into different grades. The survival period of patients with grade 1-2 is generally about 5-10 years; the survival time of patients with grade 3-4 is shorter, about 1 year. Glioma has the characteristics of high recurrence rate, high mortality rate, and low cure rate. Due to its poor differentiation, rapid proliferation, and strong invasiveness, current surgical methods cannot completely remove the tumor....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/40A61K31/155A61K45/06A61K31/4188A61P35/00
Inventor 宋明柯
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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