InDel molecular marker realizing coseparation with rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of InDel molecular marker

A technology of xa34-nv7indelrv and rice bacterial blight, applied in the field of agricultural biology, can solve the problems of low throughput, long time-consuming, expensive reagents, etc., and achieve the effects of high specificity, reduced pollution, and long detection time
CN108103237AActive Publication Date: 2018-06-01PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
Publication Date
2018-06-01

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Abstract

The invention discloses an InDel molecular marker realizing coseparation with the rice xanthomonas oryzae resistant gene xa34(t) as well as detection primers and applications of the InDel molecular marker. The InDel molecular marker has the nucleotide sequence as shown in SEQ ID NO.1. The InDel molecular marker is the co-dominant marker, can distinguish homozygotes from heterozygotes, and can be used for identifying the rice xanthomonas oryzae resistant variety. The InDel molecular marker is high in specificity, the band differences are great, and clear distinguishing can be realized through agarose gel electrophoresis. The invention further provides detection primers of the InDel molecular marker, which are composed of the primer as shown in SEQ ID NO.3 and the primer as shown in SEQ ID NO.4. The invention further provides a detection kit of the InDel molecular marker, which comprises the detection primers. With the detection primers or the detection kit, the molecular breeding process for the broad-spectrum xanthomonas oryzae resistance of rice is greatly accelerated.
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Description

technical field

[0001] The invention belongs to the field of agricultural biotechnology, and particularly relates to an InDel molecular marker co-separated with rice bacterial blight resistance gene xa34(t), detection primers and application thereof. Background technique

[0002] Bacterial blight (Xanthomonas oryzae pv. oryzae) is one of the main diseases of rice in my country and Southeast Asia, which is the main rice producing area in the world, and seriously threatens the safety of rice production. Bacterial blight infection can harm the growth of rice, generally reducing yield by 10%-30%, and can exceed 50% in severe cases, or even no harvest [Mew, 1987, Current Status and Future Prospects of Research on Bacterial Blight of Rice.Ann Rev Phytopathol , 25:359-382.]. Since the disease is a vascular bacterial disease, the control effect of common chemical agents is not ideal, and the use of host resistance is an effective means to control the disease [Wu Shangzhong, 1982, R...

Claims

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