InDel molecular marker co-segregated from ZYMV resistance gene of cucumber

Abstract

The invention relates to an InDel molecular marker co-segregated from a ZYMV resistance gene. The InDel molecular marker named InDel-zym1 and is composed of a nucleotide sequence fragment shown in SEQ ID NO. 1 and a nucleotide sequence fragment shown in SEQ ID NO. 2, wherein the nucleotide sequence fragment shown in SEQ ID NO. 1 is co-segregated from a resistance gene; the nucleotide sequence fragment shown in SEQ ID NO. 2 is co-segregated from a disease-susceptible gene. The InDel molecular marker provided by the invention has high stability and can be easily and rapidly applied to the auxiliary screening of ZYMV resistant individual plants in a cucumber seedling stage at high throughput; according to SEQ ID NO. 5 and SEQ ID No. 6, multiple pairs of co-dominant InDel markers can also be designed as molecular markers for ZYMV resistance screening on account of difference in DNA length of resistance candidate genes caused by insertion/deletion mutations. The InDel marker provided by the invention lays a foundation for ZYMV resistance molecular marker-assisted breeding, and greatly accelerates progress of breeding of ZYMV resistance molecules of cucumber.

Description

technical field [0001] The invention relates to genetic engineering technology, in particular to InDel molecular marker co-separated with cucumber ZYMV resistance gene. Background technique [0002] Cucumber (Cucumis sativus L.) is an annual herbaceous climbing plant in Cucurbitaceae. Originating in the northern part of India at the southern foot of the Himalayas, my country has a cultivation history of more than 2,000 years and is the secondary origin center of cucumbers. As one of the ten most important vegetable crops in the world, cucumber is also one of the main vegetable crops in my country. As an important vegetable crop, it has always been valued by breeders. [0003] In recent years, ZYMV (Zucchini Yellow Mosaic Virus, Cucumber Yellow Mosaic Virus) has become more and more harmful to cucumbers. After cucumbers are infected with ZYMV, the leaves appear yellowing, mosaic, and there are tumor-like protrusions on the surface of the leaves. When the disease is severe, t...

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Problems solved by technology

[0006] The above studies show that ZYMV resistance in cucumber is controlled by a single recessive gene. Some linked markers obtained so far have a long genetic distance or are not suitable for large-scale population screening, which is not conducive to the development of molecular marker-assisted breeding.

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