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an ace inhibitory peptide
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A technology for inhibiting peptides and lowering blood pressure, applied in the field of angiotensin-converting enzyme inhibitory peptides, can solve problems such as small side effects, and achieve the effect of low cost
Inactive Publication Date: 2021-05-25
BOHAI UNIV
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Problems solved by technology
However, these synthetic drugs have many side effects, compared with synthetic ACE inhibitors, ACE inhibitory peptides from natural food are safer, more stable, and have fewer side effects
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Embodiment 1
[0017] Example 1 Virtual enzymatic hydrolysis and virtual screening of large yellow croaker actin
[0018] 1. Screening of potential bioactive peptides in large yellow croaker actin.
[0019] The large yellow croaker actin sequence (KKF15719) was obtained from the NCBI database, and pepsin, trypsin and chymotrypsin were used for virtual enzymolysis. Unpublished tripeptide sequences were predicted by ToxinPred and Innovagen servers for toxicity and water solubility to obtain peptide sequences with potential biological activity, and to obtain tripeptide sequences that are non-toxic and have good water solubility.
[0020] 2. Screening of potential ACE inhibitory peptides
[0021] From the PDB database, the crystal structure of ACE (1O86 resolution 2.0 ), set it as a receptor protein by means of DS, and predict the active site for molecular docking. The tripeptide sequence obtained in step 1 was docked with ACE by Libdock. And calculate the LibDock score of the top ten optim...
Embodiment 2
[0024] Example 2 Identification of HER in vitro ACE inhibitory activity
[0025] Using high-performance liquid chromatography, take hippuryl histidyl leucine (HHL) substrate solution, add inhibitors and mix evenly, preheat in a constant temperature water bath at 37°C for 3 to 5 minutes, then add ACE solution and mix thoroughly, 37 After incubating at ℃ for 30 min, 1 mol / L HCl was added to terminate the reaction to obtain a reaction solution. At the same time, boric acid buffer solution was used instead of inhibitor solution as blank control group. The reaction solution was directly analyzed by HPLC system.
[0026] Chromatographic conditions: column temperature 25°C, flow rate 0.5mL / min, mobile phase acetonitrile / water 25:75 isocratic elution, detection wavelength 228nm.
[0027] 5. Result Analysis
[0028] Based on all the above results, a comprehensive evaluation was carried out, and the tripeptide HER was screened. HER has good ACE inhibitory activity, IC 50 The value ...
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Abstract
The invention discloses an ACE inhibitory peptide. The amino acid sequence of the ACE inhibitory peptide is His-Glu-Arg (HER). The peptide can effectively inhibit the activity of ACE, and the IC 50 The value was 1.82±0.06 mM. The ACE inhibitory peptide involved in the present invention has the characteristics of simple structure, safety, strong activity, etc., and has wide application prospects.
Description
technical field [0001] The present invention relates to the production of an angiotensin-converting enzyme (ACE) inhibitory peptide. Background technique [0002] Large yellow croaker, also known as large yellow flower, is the main economic fish in my country's coastal waters. Its meat is delicious and nutritious. Actin and myosin are the major myofibrillar proteins in fish, contributing up to 80% of the myofibrillar protein fraction. With the in-depth research on marine organisms, more and more physiologically active substances have been discovered, especially the research on peptides. The main function of large yellow croaker peptide is reflected in anti-oxidation, antibacterial and other aspects. [0003] Hypertension is a chronic disease known as the invisible killer, affecting about one-third of adults worldwide and responsible for about one-half of all deaths. Long-term hypertension is generally considered a major risk factor for coronary artery disease, peripheral...
Claims
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