Hydrogel as well as preparation method and application thereof

Abstract

The invention relates to hydrogel as well as a preparation method and application thereof. The hydrogel is patterned temperature-sensitive hydrogel which comprises the following raw material components in parts by mass: 10-50 parts of an N-isopropylacrylamide monomer, 0.01-0.2 part of graphene oxide and 5-30 parts of a cross-linking agent. The hydrogel is applied to cell culture and collection ofcell layers and can be further used for establishing three-dimensional tumor models. The hydrogel provided by the invention is reasonable in raw material component matching and excellent in temperature sensitivity; due to reasonably matched concentrations of graphene oxide, the hydrogel has hydrophobicity at a cell culture temperature, and adhesion and propagation of cells on the surface of the hydrogel can be promoted; under a room temperature condition, the hydrogel has hydrophilcity instead of hydrophobicity, peeled cell layers can be collected with a short time, the obtained cell layers can be further cultured into cell spheres of three-dimensional structures, and the cell spheres can be applied to tumor model study and used as cell models for screening anti-tumor medicines.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a hydrogel and a preparation method and application thereof. Background technique [0002] The damage and loss of the functions of human tissues and organs can lead to disease and death. According to data from the United States, more than one million people suffer from diseases that damage or lose tissue and organ functions every year. my country is the most populous country in the world, and it is also the country with the most tissue and organ dysfunction or loss of diseases. [0003] The ideal tissue engineering is to adsorb the normal tissue cells of the body on the scaffold for in vitro culture, and this scaffold can be absorbed by the body. When it reaches a certain level, the cell-scaffold is implanted into the diseased part of the body, and as the scaffold is slowly absorbed by the body , the above cells will gradually form the corresponding tissue, so ...

AI Technical Summary

Problems solved by technology

However, the adhesion ability of cells on the surface of pNIPAm hydrogel is not ideal. In order to improve the adhesion and proliferation performance of cells on the surface of pNIPAm hydrogel, materials such as lithium diatomaceous earth, chitosan, gelatin, and multi-walled carbon nanotubes were used for modification. pNIPAm hydrogel, but the prepared gel has poor flexibility and slow response rate

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