Microbial preparation and aquaculture water purification method

A technology for microbial preparations and aquaculture water, which is applied in the field of water treatment to achieve the effects of high mechanical strength, rapid and efficient degradation, and high density

Pending Publication Date: 2022-07-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the technical problems that microorganisms are greatly affected by the environment, the survival rate and content of beneficial bacteria in the composite bacterial agent are low, the biological activity and adaptability are poor, and the degradation effect of nitrite is unstable, the present invention provides a microbial agent and a method for purifying aquaculture water. The composite bacteria fixative and ferrous stabilizer used in microbial preparations are low in cost and have the ability to efficiently degrade nitrite and nitrate. They have broad application prospects in solving the problem of high nitrite in aquaculture water

Method used

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  • Microbial preparation and aquaculture water purification method
  • Microbial preparation and aquaculture water purification method

Examples

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Effect test

Embodiment 1

[0044] A microbial preparation, comprising a composite bacterial fixative and a ferrous stabilizer in a mass ratio of 2:1, the bacteria used in the composite bacterial fixative are Pseudomonas stutzeri (strain number CGMCC1.3184), rhamnose Lactobacillus (strain number is CGMCC1.533), Lactobacillus plantarum (strain number is CGMCC1.16089), Lactobacillus brevis (strain number is CGMCC1.3258), the above four kinds of bacteria can be purchased at the China General Microorganisms Collection and Management Center.

[0045] Wherein, the preparation method of composite bacterial fixative is as follows:

[0046] (1) Under stirring state, add sodium alginate and zeolite powder into distilled water in sequence in a mass ratio of 1:1, this adding method is beneficial to avoid agglomeration phenomenon, magnetic stirring 12h after adding sodium alginate and zeolite powder, Then add CaCO 3 Continue to stir evenly to obtain a premix, CaCO 3 The amount added is 0.1wt% based on the total mas...

Embodiment 2

[0067] The difference between Example 2 and Example 1 is that the ratio of four single bacterial bacterial suspensions in the composite bacterial solution is different when the composite bacterial fixative is prepared, and the composite bacterial solution of Example 2 is prepared according to the following method:

[0068] Pour the bacterial suspensions of Pseudomonas stutzeri, Lactobacillus rhamnosus, Lactobacillus plantarum and Lactobacillus brevis into a centrifuge tube at a mass ratio of 2:1:1:2, centrifuge at 10,000 rpm for 10 min, and rinse with sterile water. Mix it evenly, configure it into a bacterial suspension with uniform distribution, count the viable bacteria under the microscope, and the number of viable bacteria is 2.63 × 10 after calculation. 9 cfu / mL.

Embodiment 3

[0070] The difference between Example 3 and Example 1 is that the ratio of four single bacterial bacterial suspensions in the composite bacterial solution is different when the composite bacterial fixative is prepared, and the composite bacterial solution of Example 3 is prepared as follows:

[0071] Pour the bacterial suspensions of Pseudomonas stutzeri, Lactobacillus rhamnosus, Lactobacillus plantarum and Lactobacillus brevis into a centrifuge tube at a mass ratio of 2:1:2:1, centrifuge at 10,000 rpm for 10 min, and rinse with sterile water. Mix it evenly, configure it into a bacterial suspension with uniform distribution, count the viable bacteria under the microscope, and the number of viable bacteria is 2.33 × 10 after calculation. 9 cfu / mL.

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Abstract

The invention relates to the technical field of water treatment, in particular to a microbial preparation and an aquaculture water purification method. The microbial preparation comprises a composite bacterial fixing agent and a ferrous stabilizer, the preparation method of the composite bacterial fixing agent comprises the following steps: (1) adding a composite carrier into water and stirring to obtain a premixed solution, and the composite carrier comprises sodium alginate, zeolite powder and CaCO3; (2) adding a compound bacteria solution into the premixed solution, and stirring and mixing to obtain a mixed solution; (3) dropwise adding the mixed solution into a CaCl2 solution, and standing for crosslinking to obtain gel balls; and (4) washing the gel balls to obtain the composite bacteria fixing agent. The ferrous stabilizer is activated carbon loaded with ferrous particles. The aquaculture water purification method adopts the microbial preparation to reduce the nitrite and/or nitrate content of the aquaculture water. The microbial preparation and the aquaculture water body purification method can obtain a faster and more efficient nitrite degradation rate.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a microbial preparation and a method for purifying aquaculture water. Background technique [0002] Fishery production is of great significance in production and life. Putting a large amount of bait into aquaculture water will cause a large amount of nitrogen to accumulate. These nitrogens are converted into ammonia nitrogen, nitrite and nitrate through the action of various microorganisms, which are absorbed by algae and aquatic plants on the one hand, and nitrate on the other hand. Nitrate is converted to nitrogen by denitrification when conditions are ripe. If the water body reaches a certain state of self-purification equilibrium and there is no external interference, the nitrogen cycle in the water body will be relatively normal, and the nitrate nitrogen will remain in a stable state. However, in aquaculture water, the regularly used disinfectants will kill both ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/28C02F3/34C12R1/38C12R1/225C12R1/25C12R1/24C02F101/10C02F101/16C02F101/30C02F103/20
CPCC12N1/20C02F3/348C02F1/281C02F1/283C02F1/285C02F2103/20C02F2101/166C02F2101/105C02F2101/16C02F2101/30
Inventor 谷劲松赵晓东张艳楠王艺霖徐会连张秀君谭晓军成堃
Owner UNIV OF JINAN
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