Serum albumin nanoparticles combined with fluorescent dyes and coomassie brilliant blue as well as preparation method and application thereof in tumor diagnosis and treatment

Abstract

The invention discloses a preparation method of serum albumin (SA) nanoparticles based on fluorescent dyes (FD) and coomassie brilliant blue (CBB). The method comprises the following steps: S101: mixing a coomassie brilliant blue solution and a serum albumin solution at a mass ratio of (1 to 5) to (1 to 40), stirring and reacting for 0.5 to 15 hours; S102: post-filtering, washing, and dispersing to obtain an intermediate product; S103: adding the fluorescent dye, which accounts for 1 to 10% of the weight percentage of the intermediate product, to the intermediate product, stirring and reactingfor 0.5 to 15h; S104: filtering to obtain the serum albumin nanoparticles. The preparation method of the serum albumin nanoparticle of the invention has the advantages of simple process, strong operability and good process repeatability, and the prepared serum albumin nanoparticles combined with the fluorescent dyes and the coomassie brilliant blue have an excellent anti-tumor effect, high selectivity and small toxic and side effects.

Description

technical field [0001] The invention relates to a novel organic nano material, especially a nano material used in the medical field, in particular to a serum albumin nano particle based on the co-combination of a fluorescent dye and Coomassie brilliant blue, and a preparation method and application thereof. Background technique [0002] Tumor is one of the diseases that threaten human health and life, and chemotherapy is still the means of tumor treatment at present. However, the toxic and side effects of chemotherapy drugs used clinically cannot be ignored. Most of the currently disclosed anti-tumor nano drug particles of serum albumin are realized by loading traditional cytotoxic chemotherapy drugs. For example, Chinese patent CN201610228149.4 discloses an anti-tumor drug and MRI contrast agent to co-deliver albumin nanoparticles and its preparation method , through the electrostatic adsorption and coordination between the drug and the carrier, as well as the cross-linkin...

AI Technical Summary

Problems solved by technology

Because traditional cytotoxic chemotherapy and antineoplastic drugs often have toxic side effects on normal cells, although the concentration of these drugs in tumor cells can be increased by using targeted technology to reduce the side effects, some organs such as the liver and spleen are inevitably absorption of nanomedicines, resulting in potential hazards to these organs

[0003] In addition, Chinese patent CN201510566206.5 discloses a copper-based human serum albumin nanocomposite with near-infrared photothermal effect and its preparation method and application. By controlling the protein concentration, copper salt concentration, reaction solution pH, reaction time and reaction temperature , induce the growth of inorganic copper-based nanocomposites, and synthesize copper-based nanostructures with human serum albumin as the skeleton and wrapped copper sulfide or copper selenide nanocomposites. The copper-based human serum albumin with near-infrared photothermal effect Nanocomposites can be used in photothermal therapy to achieve efficient heat elimination of tumors. They are inorganic nanocomposites with poor biocompatibility and cannot be used for direct chemotherapy.

[0004] The preparation and application of serum albumin nanoparticles based on the co-combination of fluorescent dyes and Coomassie brilliant blue has not been found so far

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