Illumination super-resolution fluorescence microscopic imaging method and device for microstructure light

Abstract

The invention relates to an illumination super-resolution fluorescence microscopic imaging method and device for microstructure light and belongs to the field of optical super-resolution microscopic imaging and fluorescence detection. A microstructure light generation device is composed of a polygonal frustum of a prism and multiple linear lasers; the polygonal frustum of a prism is made of a transparent material, the area of the lower bottom surface is larger than that of the upper bottom surface, the included angle between each side surface and the bottom surface of the polygonal frustum ofa prism is beta, two or more side surfaces of the polygonal frustum of a prism are provided with the linear lasers, all the linear lasers can be reflectively transmitted at the beta angle in a zigzagshape between the upper and lower bottom surfaces of the frustum of a prism, partial light can be reflected and leak every time that light is vibrated to the upper bottom surface, and finally clear strip structure light can be generated on the upper surface of the device. The illumination super-resolution fluorescence microscopic imaging method and device for the microstructure light can be applied to a super-resolution fluorescence microscopic system.

Description

technical field [0001] The invention relates to a miniature structured light illumination fluorescence microscopic imaging method and device, belonging to the field of optical super-resolution microscopic imaging and fluorescence detection. Background technique [0002] Optical microscopes play a very important role in the fields of biology and medicine. The size of the microscopic ability plays an extremely important role in promoting the development of science and human thinking. Due to the existence of the emission limit of ordinary optical microscope, the horizontal resolution of imaging is about 200nm, and the vertical resolution is 500nm~600nm. [0003] With a new entry point, super-resolution optical microscopy technology breaks through the optical diffraction limit by adding temporal or spatial modulation on the basis of ordinary optical microscopes, and improves the optical resolution of the microscope to within 100nm. Among various super-resolution microscopy imag...

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Problems solved by technology

[0006] Aiming at the problems that the above-mentioned structured light generation device and structured light illumination super-resolution fluorescence microscopy system are bulky, and it is difficult to further reduce the volume, and the accuracy and time resolution are difficult to further improve, we propose a non-interferential covering structured light generation Device and Fluorescence Super-resolution Microscopy System Based on it

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