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Method for preparing high-efficiency flocculating agent by using compound microbial inoculum

A technology for compounding bacteria and flocculants, applied in microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of low flocculation activity, high cost, low yield, etc., and achieve no toxic side effects, The effect of high safety and high flocculation rate

Active Publication Date: 2018-07-03
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of microbial flocculant-producing bacteria have been screened from soil, activated sludge, domestic sewage, and various industrial wastewater environments. Among them, Aspergillus sojae AJ7002 and Takagi which produce microbial flocculant AJ7002 discovered by Nakamura et al. Paecilomyces producing PF101 and Rhodococcus erythrophylla producing NOC-1 found by Kurane et al. These microbial flocculants have the advantages of environmental friendliness and no secondary pollution compared with traditional polymer chemical flocculants, but due to Some substrates required for the production of bioflocculants, such as sucrose, glucose, and fructose, are too expensive, low in yield, and low in flocculation activity, making it difficult to scale up their application and industrialization. The promotion application is still quite different

Method used

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  • Method for preparing high-efficiency flocculating agent by using compound microbial inoculum
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  • Method for preparing high-efficiency flocculating agent by using compound microbial inoculum

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Effect test

Embodiment 1

[0036] A kind of method utilizing compound bacterial agent to prepare efficient flocculant, compound bacterial agent is made up of fast-growing bacteria strain Celeribacter sp.GHF1031 and albicans rhodobacter strain Albirhodobacter sp.GHF1032, comprises the following steps,

[0037] a) Inoculate the bacterial classifications of the fast-growing bacillus strain Celeribacter sp.GHF1031 and the albirhodobacterium strain Albirhodobacter sp.GHF1032 on the solid medium respectively, and then add sterile water to make GHF1031 strain liquid and GHF1032 bacterial strain liquid respectively; The culture temperature of the culture medium is 25 ℃, the culture time is 36 hours, the volume of the added sterile water is 1.2 times of the solid medium; The volume is 1.2 times that of the solid medium;

[0038] b) Mix the two bacterial strains to obtain a mixed bacterial liquid, and then inoculate it into a liquid medium for fermentation to obtain a fermented bacterial liquid; The inoculum con...

Embodiment 2

[0044] A kind of method utilizing compound bacterial agent to prepare efficient flocculant, compound bacterial agent is made up of fast-growing bacteria strain Celeribacter sp.GHF1031 and albicans rhodobacter strain Albirhodobacter sp.GHF1032, comprises the following steps,

[0045] a) Inoculate the bacterial classifications of the fast-growing bacillus strain Celeribacter sp.GHF1031 and the albirhodobacterium strain Albirhodobacter sp.GHF1032 on the solid medium respectively, and then add sterile water to make GHF1031 strain liquid and GHF1032 bacterial strain liquid respectively; The cultivation temperature of the culture medium is 27 ℃, and the cultivation time is 42 hours, and the volume of the added sterile water is 1.35 times of the solid medium; The volume of the solid medium is 1.35 times;

[0046] b) Mix the two bacterial strains to obtain a mixed bacterial liquid, and then inoculate it into a liquid medium for fermentation to obtain a fermented bacterial liquid; The...

Embodiment 3

[0052] A kind of method utilizing compound bacterial agent to prepare efficient flocculant, compound bacterial agent is made up of fast-growing bacteria strain Celeribacter sp.GHF1031 and albicans rhodobacter strain Albirhodobacter sp.GHF1032, comprises the following steps,

[0053] a) Inoculate the bacterial classifications of the fast-growing bacillus strain Celeribacter sp.GHF1031 and the albirhodobacterium strain Albirhodobacter sp.GHF1032 on the solid medium respectively, and then add sterile water to make GHF1031 strain liquid and GHF1032 bacterial strain liquid respectively; The culture temperature of the culture medium is 30 ℃, the culture time is 48 hours, the volume of the added sterile water is 1.5 times of the solid medium; The volume is 1.5 times that of solid medium;

[0054] b) Mix the two bacterial strains to obtain a mixed bacterial liquid, and then inoculate it into a liquid medium for fermentation to obtain a fermented bacterial liquid; The inoculum concent...

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Abstract

The invention belongs to the technical field of flocculant preparation, and discloses a method for preparing a high-efficiency flocculating agent by using compound microbial inoculum. The method comprises the steps of: strain cultivation, fermenting cultivation, sedimentation by using modified bentonite, drying and the like, wherein the compounding strain is obtained by compounding a fast-growingbacillus strain Celeribacter sp. GHF1031 and a white Rubrobacter strain Albirhodobacter sp. GHF1032 which are obtained through separation and purification of mud which is spit out by Ruditapes philippinarum. The flocculating agent which is obtained by using the corresponding method and the compound microbial inoculum which is prepared from the fast-growing bacillus strain Celeribacter sp. GHF1031and the white Rubrobacter strain Albirhodobacter sp. GHF1032 has the characteristics of a high flocculation rate, has stable performance, no toxic and side effects, high safety, simple production andlow cost at the same time, and can be used as a wastewater flocculating agent.

Description

technical field [0001] The invention relates to the technical field of flocculant preparation, in particular to a method for preparing a high-efficiency flocculant by using a compound bacterial agent. Background technique [0002] Flocculants are one of the commonly used agents in water treatment technology. In recent years, the most widely used traditional high-molecular polymer flocculants have been found to have potential problems that threaten human health and destroy the ecological environment. Research on new environmentally friendly and pollution-free flocculants has become a hot issue in water treatment research. Most of them are microbial flocculants. Bioflocculants are a class of polymers with biodegradable functions, including polysaccharides, lipids and proteins secreted by microorganisms with such special functions. Because of its safety and non-pollution to the environment, it is valued by many scientific researchers at home and abroad. At present, a variety...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C02F101/20C12R1/01
CPCC02F3/34C02F2101/20C12N1/20
Inventor 穆军杨桥冯丽娟陈庆国阳广凤
Owner ZHEJIANG OCEAN UNIV
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