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Efficient flocculating bacterium and application thereof in sewage treatment

A technology for flocculating bacteria and sewage, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., can solve problems such as carcinogenicity, water quality impact, toxicity, etc., achieve high flocculation rate, simple and efficient sewage treatment Effect

Active Publication Date: 2022-03-22
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic flocculants such as aluminum chloride, polyferric sulfate, etc. Although these substances are cheap, they are easy to introduce some metal ions and affect water quality
Organic flocculants are mostly toxic or carcinogenic

Method used

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  • Efficient flocculating bacterium and application thereof in sewage treatment
  • Efficient flocculating bacterium and application thereof in sewage treatment
  • Efficient flocculating bacterium and application thereof in sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Isolation and Screening of Bacteria with Flocculation Effect

[0038] 1. Medium:

[0039] Liquid medium: urea 1.5g / L, glucose 20g / L

[0040] Solid medium: urea 1.5g / L, glucose 20g / L, agar 20g / L

[0041] 2. Experimental steps:

[0042] (1) Weigh 10g of activated sludge sample collected from sewage plant sludge, add it to a test tube with 100mL of pure water, and stir it evenly with a magnetic stirrer;

[0043] (2) according to 10 -1 、10 -2 、10 -3 For three gradient dilutions, take 0.2mL of the diluted sample and spread it on a solid medium plate;

[0044] (3) Put the plate upside down and place it in a constant temperature incubator at 30°C for 24 hours, then pick out strains with large morphological differences, put them in a test tube containing 5 mL of liquid medium, and place them in a constant temperature shaking incubator at 30°C and 160 rpm / min Cultured in medium for more than 72h;

[0045] (4) pick single bacterium colony to obtain the single bacterium co...

Embodiment 2

[0051] Identification of flocculation strains

[0052] (1) Physiological and biochemical tests

[0053] The bacterial species were identified by Gram staining, VP test, gelatin liquefaction test, amylase test, nitrate reduction test and indigo matrix test, the results are as follows Figure 3-6 shown. And its statistics are shown in Table 1 below. In Table 1, "+" indicates that the test result is positive, and "-" indicates that the test result is negative.

[0054] Table 1

[0055]

[0056] (2) Identification of 16S rDNA gene sequence

[0057] Take 1 mL of the cultured bacterial liquid in Example 1, put it in a centrifuge tube and centrifuge, collect the bacterial cells to collect the bacterial cells, use a DNA extraction kit to extract DNA, and after electrophoresis detection, use the universal primer 27F (5'-agagtttgatcmtggctcag-3' , shown in SEQ ID NO.1) and primer 1492R (5'-ttggytaccttgttacacact-3', shown in SEQ ID NO.2) for amplification.

[0058] Sequencing result...

Embodiment 3

[0060] The flocculation effect of the sieved flocculation bacteria is evaluated by flocculation experiments of simulated aquaculture sewage. The specific implementation method is as follows:

[0061] (1) Configure simulated aquaculture sewage, the main components of which are shown in Table 1

[0062] Table 1. Composition of artificially simulated aquaculture wastewater

[0063]

[0064] (2) Take 50mL of simulated aquaculture sewage configured, and slowly add 2mL of the fermented flocculation strain separated and purified in Example 1 to it, and the bacterial concentration of the resulting mixture is 2×10 7 cfu / mL, pH is 6, stir rapidly at 200r / min for 5min, then stir at 100r / min at a slow speed for 5min to make it uniform, let it stand for 30min, measure the supernatant of the sample to be tested at 550nm with a UV-visible spectrophotometer The absorbance of the solution and the absorbance of the supernatant of the blank solution were used to calculate the flocculation ra...

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Abstract

The invention relates to the technical field of sewage treatment, in particular to a high-efficiency flocculating bacterium and application thereof in sewage treatment, and the flocculating bacterium is pseudomonas guguanensis, is named as Peseomonas guguanensis 2021XNI14, and is preserved in the China Center for Type Culture Collection (CCTCC). The flocculating bacterium can be used for preparing a microbial flocculant, is used for treating aquaculture sewage, village sewage, domestic sewage and industrial sewage, and has the advantages of high efficiency, high nitrogen removal rate and high flocculation rate. The flocculating bacterium is easy to screen, high in culture yield and easy to be used for preparing a microbial flocculant, and has a wide application prospect in the sewage treatment industry.

Description

technical field [0001] The application relates to the technical field of sewage treatment, in particular to a high-efficiency flocculation bacterium and its application in sewage treatment. Background technique [0002] The flocculants currently used in sewage treatment mainly include inorganic flocculants, organic flocculants and biological flocculants. Inorganic flocculants such as aluminum chloride, polyferric sulfate, etc. Although these substances are cheap, they are easy to introduce some metal ions and affect water quality. Most organic flocculants are toxic or carcinogenic. [0003] Microbial flocculant is a kind of highly efficient, non-toxic and non-secondary pollution water treatment agent with flocculation effect obtained by fermentation of microorganisms such as bacteria and fungi. These microorganisms are called flocculation bacteria. Compared with inorganic and organic flocculants, microbial flocculants have the following advantages: easy to use, wide range ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/04C12Q1/04C02F3/34C12R1/38
CPCC12N1/02C12N1/04C12Q1/04C02F3/34Y02W10/10
Inventor 彭霞邓兵濮振宇关亚萍秦心儿高其双邵中保吕周亚刘晓华刘武
Owner WUHAN ACADEMY OF AGRI SCI
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