Novel method for detecting taurocholic acid content and related substances

A technology of taurocholic acid and related substances is applied in the detection field of new taurocholic acid content and related substances, and achieves the effects of strong reliability, low detection cost and reduced detection cost

Active Publication Date: 2018-07-06
ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
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Problems solved by technology

This method effectively separates and accurately quantitatively detects tauro

Method used

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  • Novel method for detecting taurocholic acid content and related substances
  • Novel method for detecting taurocholic acid content and related substances
  • Novel method for detecting taurocholic acid content and related substances

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific embodiment:

[0030] The related substance described in the new taurocholic acid content and related substance detection method of the present invention is cholic acid;

[0031] 1, the chromatographic condition that the present invention adopts:

[0032] 1.1 Chromatographic column: C8 chromatographic column

[0033] 1.2 Mobile phase: After mixing according to the ratio of 0.05mol / L ammonium acetate aqueous solution: methanol: tetrahydrofuran = 5:6:2, adjust the pH of the solution to 7.50 with triethylamine.

[0034] 1.3 Diluent: mobile phase

[0035] 1.4 Flow rate: 1.0ml / min Column temperature: 35°C Injection volume: 20ul

[0036] 1.5 Detector: differential refractive index detector

[0037] 2: Method for determination of related substances and content of taurocholic acid by HPLC

[0038] 2.1 Take the taurocholic acid-TRC reference substance as a known reference substance, dissolve i...

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Abstract

The invention discloses a novel method for detecting a taurocholic acid content and related substances. The method comprises the following steps: taking a taurocholic acid-TRC reference substance as aknown reference substance, dissolving the reference substance by a diluent to form a reference substance solution, and dissolving a taurocholic acid sample by a diluent to form a reference substancesample solution; injecting 20ul of each solution into a liquid chromatograph respectively, recording a peak area, and carrying out calculation according to an external standard method on the basis ofanhydrous taurocholic acid; taking cholic acid as a reference substance, dissolving the reference substance by a mobile phase to form a concentration impurity reference substance solution, and dissolving the taurocholic acid sample by a diluent to form a concentration sample solution; injecting 20ul of each solution into the liquid chromatograph respectively, recording the peak areas of sodium taurocholate and cholic acid in a chromatogram, and calculating the sizes of related substances in the taurocholic acid sample according to the external standard method. Effective separation and accuratequantitative detection of taurochenodeoxycholic acid and chenodeoxycholic acid which are two cholic acid substances with large difference in polarities are realized under the same liquid chromatography condition.

Description

technical field [0001] The invention relates to a novel detection method for taurocholic acid content and related substances. Background technique [0002] The chemical name of taurocholic acid (TCA) is 3α, 7α, 12α trihydroxy-5β-24 cholic acid-N-taurine, the molecular formula is C26H45NO7S, and the molecular weight is 515.71. It is a combined bile acid formed by the shrinkage between the carboxyl group of cholic acid (CA) and the amino group of taurine. Taurocholic acid is the main bile acid in the bile of cattle, sheep, and snakes. Animal bile is a traditional Chinese medicine with a long history. Conjugated bile acids are the active components of bile for antitussive, expectorant and anti-inflammatory effects, and it is the main material basis for the pharmacological effects of bile. Taurocholic acid is the main component of bound bile acids in the bile of cattle and sheep, accounting for 50% to 60% of bound bile acids. The existing documents and inventions mainly inclu...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 郭然娄良吴黎雄张和平
Owner ZHONGSHAN BAILING BIOTECHNOLOGY CO LTD
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