Applications of picroside II in anti-breast cancer drugs

A technology of picroside and anti-breast cancer, which is applied to the application field of picroside II in anti-breast cancer drugs, can solve problems such as limitation, and achieve the effects of inhibiting proliferation, good anti-metastatic effect, and not easy to produce drug resistance

Inactive Publication Date: 2018-07-13
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemically synthesized drugs of Western medicine to treat cancer has a signi

Method used

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  • Applications of picroside II in anti-breast cancer drugs
  • Applications of picroside II in anti-breast cancer drugs
  • Applications of picroside II in anti-breast cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. The effect of picroside II on the viability of MDA-MB-231 cells

[0032] MDA-MB-231 cells were treated with different concentrations (0-40μM) of picroside II for 24, 48, 72h, and MTT (20μL, 5mg / mL) reagent was added, and the absorbance value was measured on a multi-function microplate reader. like figure 1 As shown, picroside II had little effect on the viability of MDA-MB-231 cells when the drug concentration was lower than 40 μM. Therefore, the above concentrations will be further used to evaluate the anti-invasion and anti-migration effects of picroside II.

[0033] 2. Picroside II inhibits the migration of MDA-MB-231 cells in vitro

[0034] To further test the in vitro anti-metastatic activity of picroside II, a wound healing assay was used in the present invention. MDA-MB-231 cells were plated at 1 × 10 per well 5 The cells were seeded in a 24-well culture plate. After culturing for 24 hours, the wound was vertically drawn with a 1000 μL pipette tip, and the...

Embodiment 2

[0046] Except that the dosage of intraperitoneal injection in the animal experiment was 40 mg / kg, other experiments and detection methods were the same as in Example 1, and the results showed that the drug had good antitumor effect.

Embodiment 3

[0048] Except that the dosage of intraperitoneal injection in the animal experiment was 60 mg / kg, other experiments and detection methods were the same as those in Example 1, and the results showed that the drug had a good antitumor effect.

[0049] In the present invention, berberine II is added with pharmaceutically acceptable auxiliary materials, and can be made into various dosage forms according to conventional methods, such as liquid injections of various specifications, powder injections, emulsions for injection, tablets, pills, capsules, ointments, Creams, patches, liniments, powders, sprays, implants, drops, suppositories, ointments, candies, etc.

[0050] The administration route of berberine II includes various administration routes, such as oral administration, injection administration, implant administration, intracavitary administration, sublingual administration, transdermal administration, internal and external application and the like.

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Abstract

The invention belongs to the field of anti-breast cancer drugs, and discloses applications of picroside II in anti-breast cancer drugs. Picroside II possesses excellent anti-metastasis effects both invivo and in vitro, is capable of inhibiting invading and metastasis of breast cancer cells, inhibiting propagation of tumor cells in vivo, is a natural compound, is low in toxic and side effect, andgenetic toxicity; drug resistance is not easily induced; a drug resource is provided for research on inhibition on tumor invading and metastasis; and a novel idea is provided for clinical treatment ofbreast cancer.

Description

technical field [0001] The present invention relates to an anti-breast cancer drug, in particular to the application of picroside II in the anti-breast cancer drug. Background technique [0002] Breast cancer is one of the most frequently diagnosed malignancies in women and is the leading cause of cancer death. Some scholars believe that breast cancer will become a major factor affecting women's health. According to the International Cancer Research Center of the World Health Organization, in 2012, new cases of breast cancer accounted for about 25.1% (1,671,149 cases) of total cancer incidence; deaths accounted for 14.7% (521,907 cases) of total cancer deaths. Similar to the situation in many other countries, breast cancer has become the most common malignant tumor among Chinese women. According to statistics, new breast cancer cases in China each year account for about 12.2% (187,213 cases) of the global total, while deaths account for about 9.6% (47,984 cases) of the tot...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P35/00A61P35/04
CPCA61K31/7048
Inventor 楼成华赵华军朱智慧陈琰
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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