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A sheep whey protein immunomodulatory peptide, its preparation method and application

A technology of immune regulation and goat whey, which is applied in the field of preparation of goat whey protein immunomodulatory peptides, can solve the problems such as few reports of goat milk-derived immunomodulatory peptide sequences, and achieve good immune regulation activity and good absorption effect , High food safety effect

Active Publication Date: 2020-03-17
HYPROCA NUTRITION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the immunomodulatory peptide sequences derived from goat milk

Method used

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  • A sheep whey protein immunomodulatory peptide, its preparation method and application
  • A sheep whey protein immunomodulatory peptide, its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A goat milk protein immunomodulatory peptide, which includes two peptide sequences: Thr-Gly-Pro-Ile-Pro-Asn-Ser-Leu (TGPIPNSL) and Ala-Pro-Glu-Pro-Glu-Val-Phe (APEPEVF ).

[0026] The preparation method of the above-mentioned goat milk protein immunomodulatory peptide is as follows:

[0027] The first step is to weigh 10g of goat whey protein, dissolve it in 200mL of water, adjust the pH to 2.0 in a water bath at 50°C, add 40KU of pepsin, and then hydrolyze for 1 hour while maintaining the temperature and pH value to obtain the hydrolysis reaction system;

[0028] The second step is to keep the temperature of the hydrolysis reaction system constant, adjust the pH value of the hydrolysis reaction system to 8.0, add trypsin 50KU, and continue hydrolysis for 2 hours; raise the temperature of the reaction system to 85°C and keep it for 15 minutes to obtain goat milk protein hydrolyzate;

[0029] The third step is to cool the goat milk protein hydrolyzate to room temperatu...

Embodiment 2

[0032] A goat milk protein immunomodulatory peptide, which includes three peptide sequences: Thr-Gly-Pro-Ile-Pro-Asn-Ser-Leu (TGPIPNSL), Arg-Phe-Phe-Asp-Asp-Lys-Ile-Ala ( RFFDDKIA) and Ala-Pro-Glu-Pro-Glu-Val-Phe (APEPEVF).

[0033] The preparation method of the above-mentioned goat milk protein immunomodulatory peptide is as follows:

[0034] The first step is to weigh 15g of goat whey protein, dissolve it in 300mL of water, adjust the pH to 3.0 in a 55°C water bath, add 50KU of pepsin, and then hydrolyze for 2 hours while maintaining the temperature and pH value to obtain the hydrolysis reaction system;

[0035] The second step is to keep the temperature of the hydrolysis reaction system constant, adjust the pH value of the hydrolysis reaction system to 8.0, add trypsin 40KU, and continue hydrolysis for 2 hours; raise the temperature of the reaction system to 85°C and keep it for 20 minutes to obtain goat milk protein hydrolyzate;

[0036] The third step is to cool the goa...

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Abstract

The invention provides goat whey protein immunoregulation peptide. The goat whey protein immunoregulation peptide contains at least one peptide sequence of Thr-Gly-Pro-Ile-Pro-Asn-Ser-Leu, Arg-Phe-Phe-Asp-Asp-Lys-Ile-Ala and Ala-Pro-Glu-Pro-Glu-Val-Phe. The goat whey protein immunoregulation peptide is obtained from goat whey protein, and the raw materials are easily available; the goat whey protein immunoregulation peptide has very obvious promotion effect on the proliferation activity of spleen lymphocyte, shows certain dose dependent effect and has immunoregulation effect. The invention also discloses a preparation method of the goat whey protein immunoregulation peptide. The preparation method has simple steps; two kinds of protease (pepsase and trypase) are combined to hydrolyze the goat whey protein, so that active peptide fragments can be released well; the goat whey protein hydrolysate is separated and purified by a gel chromatographic column and high-performance liquid chromatography, and the purity of the product is higher. The invention also provides application of the goat whey protein immunoregulation peptide. The goat whey protein immunoregulation peptide is applied to preparation of food or health-care food with immunoregulation activity, and has wide application prospect.

Description

technical field [0001] The invention relates to the field of food technology, in particular to a goat whey protein immunomodulatory peptide, a preparation method and application thereof. Background technique [0002] At present, with the development of society, problems such as environmental pollution, social pressure, and population aging are becoming more and more serious. Under the influence of these factors, the body will inevitably experience a decline in immunity, which will lead to a series of health problems. In recent years, milk-derived immune active peptides have attracted much attention. Milk-derived immunoactive peptides are short peptides separated from milk proteins by protease hydrolysis, and are the second type of biologically active peptides found in milk proteins after morphine peptides. In previous reports, multiple immunomodulatory peptide sequences have been isolated from milk, such as TTMPLY, RYLGYLE, RYLGYL, FFVAPFPEVFGK derived from milk αs1-casein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/16C07K1/14C07K1/36A23L33/18
CPCA23L33/18A23V2002/00C07K7/06C12P21/06A23V2250/55A23V2200/324
Inventor 侯艳梅毛学英刘跃辉阿尔弗雷德·亨德里克曼刘柯驿尹亚军吴红
Owner HYPROCA NUTRITION CO LTD
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