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Trichoderma brevisense gsaamlshu-1 and its application

A technology of Trichoderma brevis, CGMCCNO.15391, applied in the field of microorganisms, can solve the problems of environmental pollution, disease and insect resistance, pesticide residues, etc.

Active Publication Date: 2021-03-23
INST OF PLANT PROTECTION GANSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the long-term and large-scale use of chemical pesticides, the problems of pesticide residues, environmental pollution and resistance of diseases and insect pests have become increasingly prominent, which has brought huge risks to people's life safety and economic income.

Method used

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  • Trichoderma brevisense gsaamlshu-1 and its application
  • Trichoderma brevisense gsaamlshu-1 and its application
  • Trichoderma brevisense gsaamlshu-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0040] Example 2 Inhibition of Trichoderma brevis GSAAMLSHU-1 to corn stalk rot pathogen

[0041] 1. Test methods and investigation items

[0042] Transfer the corn stalk rot pathogens F. graminearum and Pythium inflatum preserved in the laboratory to the PDA for activation, and culture them at 25°C until the mycelia cover the plate. , use a puncher to make a cake with a diameter of 6mm, transfer the cake of Trichoderma and the cake of corn stalk rot pathogen to the two sides of the PDA plate respectively, and keep the two cakes at a distance of 4cm, and cultivate them at a constant temperature of 25°C for 5 days. Observe and record the colony radius of Trichoderma and stem rot pathogens. Inoculate only the pathogenic bacteria of stem rot but not Trichoderma as a control. Five plates were replicated per treatment. Measure the colony growth rate to calculate the mycelium inhibition rate. Wherein the PDA formula is: 200g of potatoes, 15g of glucose, 12g of agar, and 1000mL o...

Embodiment 4

[0054] The influence of embodiment 4 Trichoderma brevis GSAAMLSHU-1 on the growth and development of corn and wheat

[0055] 1. Preparation method of spore suspension

[0056] After activating the Trichoderma brevis strain GSAAMLSHU-1 stored at low temperature at 5°C, it was inoculated on PDA medium, and after growing at a constant temperature in a constant temperature incubator at 28°C for 4 days, the concentration of 1×10 was first prepared with sterile water. 7 individual / mL stock solution of spore suspension, and then successively diluted to 1×10 6 / mL and 1×10 5 spore suspension per mL for later use.

[0057] 2. Test methods and survey indicators

[0058] Healthy and uniform-sized corn varieties (Jinsui No. 3) and wheat seeds (Huixianhong) were selected, disinfected with 75% alcohol for 10 minutes, and then rinsed with sterile water for 3 times. Put the treated seeds into a sterilized petri dish with a diameter of 90mm lined with double-layer filter paper, add 10 corn...

Embodiment 5

[0067] Example 5 Effect of Trichoderma brevis GSAAMLSHU-1 on soybean growth and development

[0068] 1. Test methods and survey indicators

[0069]Before soybean planting, 50 g of solid fermented product of Trichoderma brevismi was applied in the hole, and the soybean morphological indicators were investigated at the adult soybean plant stage, including plant height, fresh weight on the ground, and number of pods.

[0070] 2. Test results

[0071] It can be seen from the test results that compared with the control, the strain has a very significant difference in the effect on soybean plant height and pod number (P<0.01), and has a certain growth-promoting effect (Table 4).

[0072] Table 4 Effects of different concentrations of Trichoderma brevisense strain GSAAMLSHU-1 on morphological indicators of wheat

[0073]

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Abstract

The present invention relates to Trichoderma brevis GSAAMLSHU-1 and application thereof. Trichoderma brevicompactum GSAAMLSHU‑1 of the present invention has a preservation number of CGMCC NO.15391. The strain GSAAMLSHU‑1 has broad-spectrum antagonism against plant pathogenic fungi, and can be used for the control of crop diseases; at the same time, because the strain is relatively safe for the growth and development of various plants, and has a certain concentration range for the growth and development of corn, wheat, and soybean. Therefore, it is expected to be used as a bacterial fertilizer to play a good role in the growth and development of crops, to overcome a series of problems caused by the excessive application of chemical pesticides, and to have broad application prospects in agricultural production.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to Trichoderma brevis GSAAMLSHU-1 and its application. Background technique [0002] With the long-term and large-scale use of chemical pesticides, problems such as pesticide residues, environmental pollution, and drug resistance of diseases and insect pests have become increasingly prominent, which have brought huge risks to people's life safety and economic income. In order to solve the pollution problem of chemical pesticides to the environment and agricultural products, countries around the world have introduced various measures to limit and reduce the use of chemical pesticides. Denmark banned the use of chemical pesticides nationwide since June 1998. The Chinese government attaches great importance to the sustainable development of agriculture. In July 1997, the State Planning Commission, the Ministry of Science and Technology and other departments included "high-efficiency...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C05F11/08C05G3/60C12R1/885
CPCA01N63/30C05F11/08C05G3/00C12N1/145C12R2001/885Y02A40/10
Inventor 郭成王春明周天旺徐生军荆卓琼
Owner INST OF PLANT PROTECTION GANSU ACAD OF AGRI SCI
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