Novel method for drug loading system constructed by means of sensitization blood platelets for treating immune thrombocytopenia

A technology for thrombocytopenia and sensitive platelets, applied in pharmaceutical formulations, allergic diseases, blood diseases, etc., can solve the problems of short curative effect and high price, and achieve the advantages of prolonged circulation time, good biocompatibility, and avoidance of toxic and side effects. Effect

Inactive Publication Date: 2018-07-31
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs such as gamma globulin and thrombopoietin are short-lived and expensive
Moreover, the risk of ITP patients due to related treatment is even greater than the risk of the disease itself

Method used

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  • Novel method for drug loading system constructed by means of sensitization blood platelets for treating immune thrombocytopenia
  • Novel method for drug loading system constructed by means of sensitization blood platelets for treating immune thrombocytopenia
  • Novel method for drug loading system constructed by means of sensitization blood platelets for treating immune thrombocytopenia

Examples

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Effect test

Embodiment 1

[0033] A new method of a drug-loading system for treating immune thrombocytopenia constructed based on sensitized platelets carrying vincristine, the method comprising the following steps:

[0034] Step 1: Add 3.2% sodium citrate solution by weight to the obtained whole blood at room temperature of 20±5°C for anticoagulation, and the volume ratio of whole blood to sodium citrate solution is 9:1 After centrifugation at 1200rpm for 5min, platelet-rich plasma was obtained;

[0035] Step 2: Platelet-rich plasma was centrifuged at 3500rpm for 10min, and washed three times with PBS buffer to make it no longer contain plasma and other blood components, and the supernatant was discarded to obtain washed platelets, and the platelet count was controlled to be (5-10)×10 6 / ul.

[0036] Step 3: Incubate 500 μl of the resuspension of washed platelets with 2.5 μl of CD41 antibody in an environment of 25±0.5° C. for 15 minutes with shaking, and centrifuge at 3500 rpm for 2 times for 5 minut...

Embodiment 2

[0045] A new method of a drug-loading system for treating immune thrombocytopenia constructed by carrying modified vindesine on sensitized platelets, the method comprises the following steps:

[0046] Step 1: Add 4% sodium citrate solution by weight to the obtained whole blood at room temperature of 20±5°C for anticoagulation, and the volume ratio of whole blood to sodium citrate solution is 10:1 After centrifugation at 1200rpm for 5min, platelet-rich plasma was obtained;

[0047] Step 2: Platelet-rich plasma was centrifuged at 3500rpm for 5min, and washed three times with PBS buffer to make it no longer contain plasma and other blood components, and the supernatant was discarded to obtain washed platelets, and the platelet count was controlled to be (5-10)×10 6 / ul.

[0048] Step 3: Incubate 400 μl of the resuspension of the washed platelets with 2 μl of CD41 antibody in an environment of 25±0.5° C. for 30 minutes with shaking, and centrifuge at 3500 rpm for 3 times for 10 m...

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Abstract

The invention discloses a novel method for a drug loading system constructed by means of sensitization blood platelets for treating immune thrombocytopenia. The method comprises the steps that firstly, whole blood is anti-coagulated and centrifuged to obtain platelet rich plasma, then, deep centrifuging washing is performed to obtain washed platelets, the washed platelets react with CD41 monoclonal antibody oscillation, and centrifuging is performed to obtain the CD41 monoclonal antibody modified target macrophage sensitization blood platelets; then, the sensitization blood platelets and an immunosuppressor or a chemically modified cytotoxic drugs are mixed and incubated, centrifuging and purifying are performed, and finally the sensitization blood platelet drug loading system carrying drug for resisting immune thrombocytopenia is obtained. The drug loading system has the advantages of being abundant in source, high in drug loading rate, good in biocompatibility, high in targeting, long in cycle time and the like, the drug can precisely target macrophage, and the concentration of the drug inside the macrophage is increased, so that the drug curative effect is improved, and meanwhile the toxic and side effect on normal tissue is effectively relieved.

Description

technical field [0001] The invention relates to a drug-carrying system, in particular to a new method of a drug-carrying system for treating immune thrombocytopenia constructed on the basis of sensitized platelets. Background technique [0002] Immune thrombocytopenia (ITP) is an acquired autoimmune disease, which is more common in women of reproductive age, with an annual incidence of about 12 / 100,000. In recent years, the incidence rate has been increasing year by year, of which refractory ITP accounts for about 1 / 10. A number of studies have shown that ITP is caused by viral infection or unexplained structural changes in platelets to produce autoanti-platelet antibodies, and platelets sensitized by antibodies are destroyed in macrophages in the liver and spleen, resulting in peripheral thrombocytopenia. The mononuclear macrophages involved in the destruction of platelets mainly function in three ways: (1) endocytosis of sensitized platelets and activation of the compleme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/51A61K47/46A61K45/00A61K31/475A61P7/04A61P37/02
CPCA61K31/475A61K45/00A61K47/46A61K47/51A61P7/04A61P37/02
Inventor 许佩佩欧阳建陈兵汪帆柳旭左华芹
Owner NANJING DRUM TOWER HOSPITAL
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