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A colloidal gold rapid detection test device for pyrazolone antipyretic and analgesic drugs, its preparation method and application

A technology for antipyretic and analgesic and pyrazolone, which is applied in the field of biological detection, can solve problems such as inability to detect rapidly on site, and achieve the effects of being economical, fast, easy to manufacture, and convenient to use

Active Publication Date: 2020-08-28
广东省药品检验所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] In order to overcome the problem that the current technology cannot detect rapidly on the spot, the purpose of the present invention is to provide a colloidal gold detection device and its method that are easy to use, fast and easy, high in sensitivity and accuracy, and can measure pyrazolone antipyretic and analgesic drugs on site. Preparation method and use

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  • A colloidal gold rapid detection test device for pyrazolone antipyretic and analgesic drugs, its preparation method and application
  • A colloidal gold rapid detection test device for pyrazolone antipyretic and analgesic drugs, its preparation method and application

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preparation example Construction

[0052] The preparation method of the colloidal gold detection device for the above-mentioned pyrazolone antipyretic and analgesic drugs, comprising the following steps:

[0053] A nitrocellulose membrane is prepared, and a detection line and a quality control line are formed on the nitrocellulose membrane; the detection line is a pyrazolone antipyretic and analgesic drug that can be combined with a monoclonal antibody of a pyrazolone antipyretic and analgesic drug The conjugated antigen is prepared by linear spotting on nitrocellulose membrane; the quality control line is the rabbit anti-mouse antibody that can bind to the monoclonal antibody of pyrazolone antipyretic and analgesic drug, which is prepared on nitrocellulose membrane. prepared by linear spotting;

[0054] Assemble the test strip: Lap and paste the sample pad, nitrocellulose membrane and absorbent paper on the bottom plate in turn in the same direction;

[0055] Preparation of reaction cups: The reaction cups co...

Embodiment 1

[0072] Example 1 Preparation of haptens of pyrazolone antipyretic and analgesic drugs

[0073] In a 100 mL three-necked flask, 3.53 g of Sulpyrine, 1.52 g of bromoacetic acid, and 0.1 g of sodium carbonate were sequentially added, and the reaction was carried out at 90° C. in an acetonitrile solution overnight. After rotary evaporation, water was added, the solution was adjusted to slightly acidic (pH 5.5-6.8) with dilute hydrochloric acid, extracted with ethyl acetate for 2-3 times, the organic phases were combined, evaporated to dryness, mixed with samples, and purified by column to obtain pyrazolone Antipyretic and analgesic drug hapten, its chemical structure is:

Embodiment 2

[0074] Example 2 Synthesis of pyrazolone antipyretic and analgesic drug immune antigen

[0075] Immune antigens were prepared from haptens of pyrazolone antipyretic and analgesic drugs. Dissolve 0.1 mmol of the hapten of pyrazolone antipyretic and analgesic drug in 2 mL of DMF, and add 0.2 mmol of DCC and 0.15 mmol of NHS with stirring. The reaction was performed overnight at 4°C under magnetic stirring. After centrifugation, the supernatant was A solution, and 140 mg of limpet hemocyanin (KLH) was weighed and dissolved in 10 mL of PBS (pH 8.0) with a concentration of 0.1 mol / L. Add 1 mL of DMF, stir and dissolve to prepare solution B. Under magnetic stirring, solution A is gradually dropped into solution B, and the reaction is carried out at 4°C for 12 hours. After centrifugation, the supernatant was taken and dialyzed against normal saline for 3 days at 4°C, and the dialysate was replaced three times a day. The obtained whole antigen was dispensed into 0.5 mL centrifuge tub...

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Abstract

The invention discloses a colloidal gold rapid detection device for pyrazolone antipyretic and analgesic drugs, and a preparation method and a use thereof. The device comprises two parts: a pyrazoloneantipyretic and analgesic drug colloidal gold test strip and a reaction cup. The pyrazolone antipyretic and analgesic drug colloidal gold test strip consists of a base plate, sample pads laid in thesame direction on the base plate successively, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane is labeled with a detection line T line and a quality control line C line. The reaction cup comprises a pyrazolone antipyretic and analgesic drug colloidal gold labeled antibody. The colloidal gold detection device can effectively detect the pyrazolone antipyreticand analgesic drugs (such as analgin, aminopyrine, antipyrine, isopropylantipyrine, 4-methylaminopyrine and the like), and has the advantages of good specificity, high sensitivity, simple operation,realization of on-site rapid detection and the like, and can better meet the requirements of on-site supervision and law enforcement of supervision departments and detection agencies.

Description

technical field [0001] The patent of the present invention discloses a colloidal gold detection device and a preparation method thereof, aiming to provide a fast, simple, high-sensitivity, pyrazolone antipyretic and analgesic drug (such as Dipyrone, Aminopyrine, Antipyretic, etc.) that can be detected simultaneously. Rapid screening kits for pyrin, ipraantipyrine, 4-methylaminoantipyrine, etc.), which are suitable for the on-site determination of pyrazolone antipyretic and analgesic drugs illegally added to health products, and belong to biological detection technology field. Background technique [0002] In recent years, illegal additions to health products such as Chinese patent medicines, health food and food have emerged one after another. Among them, analgesic health products have become the hardest hit by illegal additions and have always been a research hotspot in the field of analysis. [1-3] . Because antipyretic, analgesic and anti-inflammatory drugs are a class o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 温家欣王炳志严义勇何嘉雯刘丛丛
Owner 广东省药品检验所
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