Tissue chip making system based on image recognition and positioning and chip making method thereof

Abstract

The invention discloses a tissue chip making system based on image recognition and positioning and a chip making method thereof. The chip making system comprises a cutting system and a computer control system; the cutting system comprises a numerical control cutting machine, an XYZ axis translation work table, a 360-degree-rotation rotary table, a receptor wax block frame, a freezing table and animage recognition and positioning module. The chip making process of the chip making system comprises sample placing, sample position identification, tissue sample image acquisition, tissue sample image processing, setting of cutting parameters, cutting of a tissue sample, tissue chip information identification and storage, and obtaining of the tissue chip with traceability of information. The method obtains a chip taking area by visual identification, has the advantages of high degree of automation and high work efficiency, through linear cutting of the tissue sample, tissue damage is effectively reduced, and the chip taking efficiency and success rate are improved; a database is established for management of the information of the tissue chip, so as to be easy to follow-up queries.

Description

technical field [0001] The invention relates to a finger core system and a core making method, in particular to a tissue chip core making system through image recognition and positioning and a core making method using the core making system, belonging to the technical field of biological and medical instruments. Background technique [0002] Tissue arrays, also called tissue microarrays (TMAS), are multiple different tissue samples placed on a single receptor block (receiver block) or paraffin block. Slice the receptor block (receiving block) according to the usual method with a microtome to obtain a specimen. [0003] Tissue microarray technology is a major innovation in experimental technology. Clinical pathologists and biologists use slices of arrayed biological tissues and pathological specimens for analysis to obtain key information on the status and changes of high-throughput biological tissues and pathological specimens. [0004] In the process of fabricating tissue ...

AI Technical Summary

Problems solved by technology

Repetitive operations such as sampling and tissue core trimming are time-consuming and labor-intensive when preparing multi-sample tissue arrays

At present, there are still the following problems in the tissue chip preparation system and method commonly used in the market: First, the current tissue chip preparation uses a sampling needle for sampling, such as: Chinese utility model application number: 201620069234.6; authorization announcement number: CN 205317515U. Sampling by pressing and rotating, although the impact on the donor tissue block or wax block is small, it is difficult to ensure the integrity of the tissue core taken, and the prepared wax core has serious problems such as tissue compression, deformation, and fracture; in addition, the sampling needle The sampling depth is not easy to control. Although the existing mechanical sampling device can accurately control the puncture depth, when sampling thinner samples, the front end of the wax core may be a blank wax core, so the tissue core in the prepared tissue chip is not located on the same plane. After that, there are vacancies in the chip array, which reduces the chip manufacturing efficiency and yield

Secondly, such as China’s invention, application number: 201310396643.8; application publication number: CN 103439163A The loose-type tissue chip preparation instrument and method used in the wax mold need manual operation in the process of inserting the wax core, and it has not yet achieved complete automation and accuracy. It is also difficult to guarantee; at the same time, the result of wax core addressing in this invention needs to be manually coded and recorded, which is inefficient and difficult to ensure accuracy

In the end, very few automatic tissue chip preparation instruments with image recognition came out, but all of them were used for needle samplers, and their own single two-dimensional image recognition monitoring system could not completely control the coring of wire cutting, only Select the sampling area from the upper part of the wax block, and the sampling depth cannot be selected from the side, which is prone to blank wax cores, and ultimately cannot take into account the degree of automation and the quality of coring

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