System and method for segmentation of three-dimensional microscope images
An imaging system and image technology, applied in image analysis, image enhancement, image data processing and other directions, can solve problems such as differential segmentation results
Active Publication Date: 2018-08-31
MOLECULAR DEVICES
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Direct methods (e.g., automatic thresholding methods) can produce poor segmentation results due to relatively low signal-to-noise ratio and densely packed objects
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[0023] refer to figure 1 , as will be apparent to those skilled in the art, the HCIS 100 may include an X-Y stage 102, one or more objective lenses 104, one or more illumination sources 106, one or more filters 108, an image capture device 110 and a controller 112 . HCIS 100 may also include one or more mirrors 114 that direct light from illumination source 106 to sample tray 116, which may be positioned on X-Y stage 102, and direct light from such sample tray 116 to the image capture device 110 . Typically, sample tray 116 includes a plurality of wells 118 , and a sample (eg, biological cells) to be imaged by HCIS 100 may be disposed in each such well 118 .
[0024] Although figure 1 Light from illumination source 106 is shown reflected from sample tray 116 to image capture device 110, but it is understood that additional mirrors (not shown) could be used so that light from illumination source 106 is transmitted through sample tray 116 and towards the image Capture device...
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A system and method to segment an image captured from an image capture device of a high content imaging system includes an image acquisition module that receives the image captured by the image capture device. A coarse object detection module develops a coarse segmented image, wherein each pixel of the coarse segmented image is associated with a corresponding pixel in the captured image and is identified as one of an object pixel and a background pixel. A marker identification module selects at least one marker pixel from the pixels of the coarse segmented image, wherein each marker pixel is one of a contiguous group of object pixels in the coarse segmented image that is furthest from a background pixel relative to neighboring pixels of the group. An object splitting module that comprisesa plurality of processors operating in parallel that associates each object pixel of the coarse segmented image with a marker pixel, wherein a distance based metric between the object pixel and the marker pixel is less than the distance based metric between the object pixel and any other marker pixel in the coarse segmented image.
Description
[0001] Related Application Cross Reference [0002] This application claims priority to US Application No. 15 / 003,302, filed January 21, 2016, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] The subject matter relates to high-content image systems, and more particularly, to systems and methods for automatically analyzing and segmenting images obtained using such systems. Background technique [0004] High-content imaging systems (HCIS) can be used to obtain microscopic images of biological samples. Such an image may include multiple cells against the background field. Further, HCIS can be used to obtain a series of microscope images of a biological sample, where each image is obtained, for example, using a different focal point. This series of microscope images can be combined to generate a three-dimensional view of a biological sample. Such series of microscope images can also be analyzed to segment and identify a po...
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IPC IPC(8): G06T7/11G06T7/136
CPCG06T7/11G06T7/136G06T2207/10061G06T2207/10012G06T2207/30024G06T7/0012G06T2207/10056G06T2207/30204G06V20/695
Inventor 洪迪慧阿夫鲁·科恩
Owner MOLECULAR DEVICES