Unlock instant, AI-driven research and patent intelligence for your innovation.
A liver cancer targeting ruthenium complex and its preparation method and application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A ruthenium complex, liver cancer technology, applied in the field of medicine, can solve problems such as lack of tumor targeting, achieve good anti-cancer effect, improve water solubility, and good biocompatibility.
Active Publication Date: 2021-08-03
SHENZHEN UNIV
View PDF9 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] In view of the deficiencies in the prior art above, the purpose of the present invention is to provide a liver cancer-targeting ruthenium complex and its preparation method and application, aiming to solve the problem that the existing metal anti-tumor drugs lack tumor targeting
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0040] The present invention also provides a preferred embodiment of the preparation method of the aforementioned liver cancer-targeted ruthenium complex, the preparation process is as follows: figure 1 shown, including:
[0041] Step A, synthetic peptide
[0042] Step B, connecting β-alanine to the N-terminus of the polypeptide to obtain
[0043] Step C, adding the carboxyl-containing ruthenium complex and the product obtained in step B to the reactive agent for reaction, and purifying the product to obtain
[0044] The present invention selects a ruthenium complex (Ru) with an active carboxyl structure (-COOH) and a polypeptide with liver cancer targeting, and designs between the two, which can improve the water-soluble β-alanine of the product, and finally synthesizes a protein with liver cancer targeting Oriented ruthenium complexes (Ru-T-Pep).
[0045] In the present invention, the polypeptide can be of any structure type, and a preferred polypeptide is HCBP1 (se...
Embodiment 1
[0053] Embodiment 1 (reaction process is as figure 2 shown)
[0054] (1) Solid-phase synthesis of polypeptides is prepared by Rink-Amide-MBHA-Resin resin (10eq, degree of substitution: 0.53mmol / L). Nitrogen gas is passed through at room temperature, and the reaction is carried out by bubbling. The amino acids with Fmoc protection are sequenced FQHPSFI sequential synthesis of peptides.
[0055] (2) Using HCTU (1eq) and DIPEA (0.5eq) as a coupling agent, link the β-Ala amino acid to the N-terminus of the above polypeptide to obtain FQHPSFI-β-A-NH 2 , mass confirmed that the target product with m / z 473.5 was obtained.
[0056](3) Condensation reaction The product of step (2) is mixed with ruthenium complex (1.2eq), HoBt (3eq), PyBop (3eq) and DIPEA (3eq), and the reaction is carried out under nitrogen for 10 hours. In the ruthenium complex The carboxyl group of β-alanine undergoes a coupling condensation reaction with the amino group in β-alanine. The above crude product was...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a liver cancer-targeting ruthenium complex and its preparation method and application. The general structural formula of the liver cancer-targeting ruthenium complex is: wherein, is a ruthenium complex; and is a polypeptide group. Tests show that the liver cancer-targeting ruthenium complex of the present invention can selectively target liver tumor cells, that is, it has a good anticancer effect on liver cancer cells, but basically has no toxicity to normal cells.
Description
technical field [0001] The invention relates to the technical field of medicine, in particular to a liver cancer-targeting ruthenium complex and its preparation method and application. Background technique [0002] Malignant tumors are obviously tissue invasive, causing tumor tissue to be mixed with normal tissue. Currently, non-targeted chemotherapy in clinical practice often causes damage to normal tissue, causing huge toxic and side effects to patients. Only targeted therapy can achieve this goal. optimal therapeutic effect. Because targeted drugs can selectively gather at the tumor site, and then specifically kill tumor cells without affecting the normal tissues around the tumor, thereby improving efficacy and reducing side effects. [0003] Currently used metal anti-tumor drugs, such as cisplatin and oxaliplatin, lack tumor targeting, which leads to serious side effects and great pain for patients during treatment. [0004] Therefore, the prior art still needs to be i...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More