A kind of improved experimental buffer and its application

A buffer and experimental technology, applied in the field of biological detection, can solve problems such as false positives, and achieve the effect of reducing electrostatic force, reducing non-specific reaction, and reducing non-specific binding

Active Publication Date: 2020-10-09
GUANGZHOU FENGHUA BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the above method is used, there are still many false positive results in the clinical expansion application.

Method used

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  • A kind of improved experimental buffer and its application
  • A kind of improved experimental buffer and its application
  • A kind of improved experimental buffer and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] An embodiment of the improved experimental buffer of the present invention comprises the following components: Tris-HCl buffer, sodium chloride, 8-anilinonaphthalene-1-sulfonate, thimerosal, calf serum, casein, Na 2 EDTA and Procline300;

[0030] The mass volume ratio of the sodium chloride is 2.55%; the mass volume ratio of 8-anilinonaphthalene-1-sulfonate is 0.08%; the mass volume ratio of thimerosal is 0.02‰; the volume ratio of calf serum is 10% ; The mass volume ratio of casein is 0.2%; Na 2 The mass volume ratio of EDTA is 0.001%; ​​the volume ratio of Procline300 is 0.1%.

[0031] The preparation method of the improved experimental buffer is as follows: prepare according to the preparation amount of 1L, accurately weigh 6.06g Tris, 0.01g Na 2 Add EDTA, 2.0g casein, 25.50g sodium chloride, 0.8g 8-anilinonaphthalene-1-sulfonate, 0.02g thimerosal into a clean container, add 300mL purified water to fully dissolve the solid reagent, slowly add 3.0mL Mix well with H...

Embodiment 2

[0033] An embodiment of the improved experimental buffer of the present invention comprises the following components: PB buffer, sodium chloride, 8-anilinonaphthalene-1-sulfonate, calf serum, bovine serum albumin, Na 2 EDTA and Procline300;

[0034] The mass volume ratio of the sodium chloride is 10.00%; the mass volume ratio of 8-anilinonaphthalene-1-sulfonate is 0.2%; the volume ratio of calf serum is 10%; the mass volume ratio of bovine serum albumin 0.2%; Na 2 The mass volume ratio of EDTA is 0.001%; ​​the volume ratio of Procline300 is 0.1%.

[0035] The preparation method of the improved experimental buffer is as follows: prepare according to the preparation amount of 1L, accurately weigh 2.32g disodium hydrogen phosphate dodecahydrate, 0.44g sodium dihydrogen phosphate dihydrate, 0.01g Na 2 Add EDTA, 2.0g bovine serum albumin, 100.0g sodium chloride, 2.0g 8-anilinonaphthalene-1-sulfonate into a clean container, add 300mL purified water to fully dissolve the solid reag...

Embodiment 3

[0037] An embodiment of the improved experimental buffer of the present invention comprises the following components: MOPS buffer, sodium chloride, sodium salicylate, mouse serum, tryptone, Na 2 EDTA and Procline300;

[0038] The mass volume ratio of described sodium chloride is 1.60%; The mass volume ratio of sodium salicylate is 0.5%; The volume ratio of mouse serum is 10%; The mass volume ratio of tryptone is 0.2%; 2 The mass volume ratio of EDTA is 0.001%; ​​the volume ratio of Procline300 is 0.1%.

[0039] The preparation method of the improved experimental buffer is as follows: prepare according to the preparation amount of 1L, accurately weigh 10.46g 3-(N-morpholine)propanesulfonic acid, 8.50ml triethanolamine, 0.01g Na 2 Add EDTA, 2.0g tryptone, 16.0g sodium chloride, 5.0g sodium salicylate into a clean container, add 300mL purified water to fully dissolve the solid reagent, then add 100mL mouse serum and 1.0mL Procline300 to make up the purified water to 1000mL, mix...

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Abstract

The invention discloses an improved experimental buffer solution. The improved experimental buffer solution comprises a basic experimental buffer solution, sodium chloride and a dissociation agent, wherein the dissociation agent is at least one selected from the group consisting of 8-anilinonaphthalene-1-sulfonate, thimerosal, sodium salicylate and sodium trichloroacetate. The invention also discloses the application of the improved experimental buffer solution. The improved experimental buffer solution provided by the invention can effectively reduce electrostatic force, promote the dissolution and dissociation of non-specific immunoreactive or non-specifically adsorbed low-affinity protein ligands, and reduce the non-specific binding of antigens and antibodies; and the improved experimental buffer solution can promote the dissociation of non-specific immunoreactive or non-specifically adsorbed protein ligands under the action of the dissociation agent, so non-specific reactions of immunoassay are reduced, and detection resolution, accuracy and precision are improved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to an improved experimental buffer and application thereof. Background technique [0002] Immunoassay is an analytical method that uses antigen-antibody specific binding reactions to detect various substances (drugs, hormones, proteins, microorganisms, etc.), and has the advantages of high sensitivity, strong specificity, rapidity and low cost; it can be used for a large number of samples Routine analysis; it can be used for qualitative screening of samples, and can also be used for quantitative determination of samples to determine the content of components to be measured in samples. Labeled immunoassay is the use of tracer substances such as fluorescein, isotope or enzyme to label antibodies (or antigens) for antigen-antibody reactions. The purpose of monitoring the immune response is achieved by measuring the markers in the immune complex. The main types...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/531G01N33/576G01N33/68
CPCG01N33/53G01N33/531G01N33/5761G01N33/5764G01N33/6893
Inventor 谭玉华刘灿赵凡一毛静宜
Owner GUANGZHOU FENGHUA BIOENG
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