Preparation method and application of nucleic acid aptamer modified gold nanostars

A nucleic acid aptamer, gold nanotechnology, applied in measurement devices, instruments, scientific instruments, etc., to achieve the effects of good stability, promotion of modification effect, and scientific and reasonable design

Inactive Publication Date: 2017-12-08
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, gold nanostars are multi-branched nanoparticles with cutting-edge structures, which have attracted extensive attention due to their convenient synthesis, high photothermal conversion efficiency, and high surface-enhanced ramen effect. The application in the field of biosensors for residue detection has not been reported yet

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  • Preparation method and application of nucleic acid aptamer modified gold nanostars
  • Preparation method and application of nucleic acid aptamer modified gold nanostars
  • Preparation method and application of nucleic acid aptamer modified gold nanostars

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Embodiment

[0034] The embodiment of the present invention provides a method for preparing a nucleic acid aptamer-modified gold nanostar, and the specific steps include:

[0035] (1) Preparation of gold nanostar solution: prepare 4-hydroxyethylpiperazineethanesulfonic acid solution and 0.01% (w / v) chloroauric acid solution with a pH value of 7.6 and a concentration of 40mmo / L respectively, and measure 15mL 4-Hydroxyethylpiperazineethanesulfonic acid solution, added to 0.3mL chloroauric acid solution, incubated at room temperature for 40 minutes, and finally, the obtained incubation product was stored in a brown reagent bottle and placed in a 4 Store in refrigerator;

[0036] (2) Modification of the nucleic acid aptamer of gold nanostars: configure 1% sodium dodecyl sulfate solution, 1Mol / L sodium chloride solution, PBS solution with a pH value of 7.4, and reconstituted solution, respectively, The solution is a mixed solution comprising 20nMol / L of sodium phosphate, 5% bovine serum albumi...

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Abstract

The invention discloses a preparation method and application of nucleic acid aptamer modified gold nanostars. The preparation method comprises the steps of: sequentially adding dATP solution and lauryl sodium sulfate solution into gold nanostar solution to oscillate and incubate; then adding sodium chloride solution at intervals repeatedly to regulate ion strength; then adding sulfydryl modified nucleic acid aptamer to carry out incubation and modification; and finally, carrying out centrifugation to obtain nucleic acid aptamer modified gold nanostar sediments. The nucleic acid aptamer modified gold nanostars is applied in pesticide residue detection in mode that by assembling a chromatographic test strip based on the competition law and detecting a combination object of the nucleic acid aptamer modified gold nanostars, judgement is carried out to obtain a pesticide residue result. According to the method provided by the invention, the nucleic acid aptamer modified gold nanostars can be efficiently, rapidly and conveniently prepared; the obtained nucleic acid aptamer modified gold nanostars are high in stability and high in bioactivity; the provided method which uses the chromatographic test strip for pesticide residue detection is reliable in principle, scientific and reasonable in design, simple, convenient and rapid to detect, high in efficiency and wide in application prospect.

Description

technical field [0001] The invention relates to the field of functional nanobiological materials, in particular to a preparation method and application of nucleic acid aptamer-modified gold nanostars. Background technique [0002] Pesticides play an important role in agricultural production. Reasonable use can effectively control pests, eliminate weeds, and increase crop yields; however, the indiscriminate use and abuse of pesticides have brought many hazards to the ecological environment and human health. In recent years, the food safety issue that consumers are most concerned about when purchasing agricultural products such as fruits and vegetables is pesticide residues. Therefore, the supervision and testing of pesticide residues in food with the allowable amount of pesticide residues as the limit is of great significance to ensure food safety in our country . [0003] In the prior art, the detection methods of pesticide residues mainly include gas chromatography, liquid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/558
CPCG01N33/531G01N33/558
Inventor 罗云波许文涛程楠黄昆仑徐瑗聪杨湛森贺晓云
Owner CHINA AGRI UNIV
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