Extraction and preservation method of mulberry leaf active substance

A technology of active substances and preservation methods, which is applied in the field of extraction and preservation of mulberry leaf active substances, can solve problems such as difficulty in maintaining the stability of active substances, cost or safety, difficulty in meeting large-scale production, and single components of active substances. Achieve the effects of low price, good economy and environmental protection, and simple process

Active Publication Date: 2021-08-31
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the new solvents that have been reported include supercritical fluids and ionic liquids, it is difficult to meet the requirements of large-scale production due to cost or safety issues, and the extracted active substance has a single component, making it difficult to maintain the stability of the active substance

Method used

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  • Extraction and preservation method of mulberry leaf active substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting and preserving active substances of mulberry leaves, comprising the following steps:

[0026] Weigh choline chloride and glycerin, mix according to the molar ratio of 1:2, the total mass is 100g, add them to the round bottom flask in turn, put them in a water bath at 80°C and keep stirring for 0.5h to form a transparent liquid. Add the synthesized solvent to 100g of water, weigh 50g of mulberry leaf powder and add it to the above-prepared solvent, extract in a 60°C water bath with ultrasonication at 150W for 0.5h, after ultrasonication, centrifuge the mixture at 12000rpm / min for 10min, and take the supernatant for detection and analysis , the measured yield of mulberry polyphenols in the extract was 206.2 mg / g, the yield of flavonoids was 73.9 mg / g, the yield of polysaccharides was 42.3 mg / g, and the yield of alkaloids was 4.5 mg / g. The extraction mixture was stored at 25° C., 4° C. and -20° C., and the content of the above-mentioned active subst...

Embodiment 2

[0028] A method for extracting and preserving active substances of mulberry leaves, comprising the following steps:

[0029] Weigh choline chloride and glucose, mix according to the molar ratio of 1:1, the total mass is 90g, add them to the round bottom flask in sequence, put them in a water bath at 80°C and keep stirring for 1 hour, and then form a transparent liquid. Add the synthesized solvent to 10g of water, weigh 30g of mulberry leaf powder and add it to the above-prepared solvent, extract it in a 60°C water bath with ultrasonication at 150W for 0.5h, after ultrasonication, centrifuge the mixture at 12000rpm / min for 10min, and take the supernatant for detection and analysis , the measured yield of mulberry polyphenols in the extract was 186.2 mg / g, the yield of flavonoids was 63.4 mg / g, the yield of polysaccharides was 35.6 mg / g, and the yield of alkaloids was 4.1 mg / g. The extraction mixture was stored at 25° C., 4° C. and -20° C., and the content of the above-mentioned...

Embodiment 3

[0031] A method for extracting and preserving active substances of mulberry leaves, comprising the following steps:

[0032] Weigh choline chloride and trehalose, mix according to the molar ratio of 2:1, the total mass is 100g, add them to the round bottom flask in turn, put them in a water bath at 80°C and keep stirring for 3 hours, and then form a transparent liquid. Add the synthesized solvent to 50g of water, weigh 30g of mulberry leaf powder and add it to the above-prepared solvent, extract it in a 60°C water bath with ultrasonication at 150W for 0.5h, after ultrasonication, centrifuge the mixture at 12000rpm / min for 10min, and take the supernatant for detection and analysis , the measured yield of mulberry leaf polyphenols in the extract was 253.2 mg / g, the yield of flavonoids was 92.9 mg / g, the yield of polysaccharides was 55.3 mg / g, and the yield of alkaloids was 5.6 mg / g. The extraction mixture was stored at 25° C., 4° C. and -20° C., and the content of the above-ment...

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Abstract

The invention discloses a method for extracting and preserving active substances of mulberry leaves. The method comprises the following steps: mixing a natural deep eutectic solvent with water as an extraction solvent, adding mulberry leaves, heating and extracting at 60-90°C for 0.5-3.0 h, then centrifuge to get the supernatant, which contains active substances such as polyphenols, flavonoids, polysaccharides and alkaloids; . The method of the invention utilizes the natural deep eutectic solvent of the plant source components as the extraction solvent and stabilizes the auxiliary extraction active substance, the extraction active substance has a broad spectrum, and the obtained product has properties such as natural safety. At the same time, the present invention utilizes the hydrogen bond interaction in the natural deep eutectic solvent to improve the stability of the active substance, which is a technical inspiration that does not exist in the prior art.

Description

technical field [0001] The invention relates to a method for extracting and preserving active substances of mulberry leaves. Background technique [0002] Organic solvents are generally used as extractants to obtain active ingredients from animals, plants or microorganisms. Due to the polar nature of organic solvents, it is difficult to maintain the stability of broad-spectrum active substances. At the same time, problems such as organic solvents are volatile, toxic, and solvent residues lead to technical bottlenecks in large-scale production. With the improvement of people's food and drug safety awareness, the use of traditional organic solvents in production has been controversial, and the development of new safe and green solvents is an inevitable trend to solve this urgent problem. [0003] Although the new solvents that have been reported include supercritical fluids and ionic liquids, it is difficult to meet the requirements of large-scale production due to cost or sa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/105A23L3/36
CPCA23L3/36A23V2002/00A23L33/105A23V2250/21
Inventor 周鹏飞邝哲师王旭苹唐翠明戴凡炜潘木水王振江黄静
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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