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Primer combination for detection of methylation of DNA under detection, method of detecting methylation of DNA under detection and application of primer combination

A primer composition and methylation technology, applied in the direction of recombinant DNA technology, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of limited diagnostic value of triple-negative breast cancer and other problems

Inactive Publication Date: 2018-09-14
CANCER HOSPITAL AFFILIATED TO HOSPITAL XINJIANG MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the histopathological diversity of triple-negative breast cancer, the heterogeneity of the same pathological tumor cells, and the complexity of tumor biological behavior, the current routine single-gene detection has limited diagnostic value for triple-negative breast cancer.

Method used

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  • Primer combination for detection of methylation of DNA under detection, method of detecting methylation of DNA under detection and application of primer combination
  • Primer combination for detection of methylation of DNA under detection, method of detecting methylation of DNA under detection and application of primer combination
  • Primer combination for detection of methylation of DNA under detection, method of detecting methylation of DNA under detection and application of primer combination

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Experimental program
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Embodiment

[0044] Embodiment: The sequences involved in the methylation testing method provided in the embodiment of the present invention are shown in Table 1.

[0045] Table 1: Sequence

[0046]

[0047] The methylation primers provided in the examples of the present invention and each set of specific amplification primer sequences in the methylation primer combination composed of methylation primers can specifically capture the target gene in the sample to be tested, and through the PCR reaction Amplify the target gene, and then obtain the methylation level of the target gene.

[0048] Embodiment: 1, sample collection and preservation

[0049] Three-negative breast cancer and benign lesions (breast fibroadenoma) tissue samples of different clinical stages were collected, one was used for immunohistochemical identification, and the other was embedded in foil paper and frozen with liquid nitrogen, and stored at -80°C.

[0050] 2. Immunohistochemical screening

[0051] Take tumors ...

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Abstract

The invention relates to a primer combination for the detection of methylation of DNA under detection, comprising primer pairs, wherein, the primer pair A includes primer 1 (as shown in the sequence SEQ 1 and primer 2 (as shown in sequence SEQ2; the primer pair B includes primer 3 (as shown in SEQ 3) and primer 4 (as shown in SEQ 4); the primer combination is applicable to the early diagnosis of triple-negative breast cancer.

Description

technical field [0001] The invention relates to the field of specific primers for detecting triple-negative breast cancer, in particular to a primer composition, a detection method and an application for detecting DNA methylation to be tested. Background technique [0002] Triple-negative breast cancer is a malignant tumor that occurs in the glandular epithelium of the breast. The immune system can use immune surveillance to target tumor-specific antigens, activate T cell-mediated anti-tumor immune responses, and achieve the purpose of disease control. Human leukocyte antigen (HLA) is an important part of the body's immune surveillance system, mediates the antigen processing machinery (APM) functional pathway, and presents the antigen-peptide complex produced in the cell to the cell surface, It makes it possible for cytotoxic T lymphocytes (cytotoxic T lymphocytes, CTL) to recognize endogenous antigenic peptides in cells, which is an important prerequisite for anti-tumor ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/118C12Q2600/154
Inventor 木妮热·木沙江艾秀清古丽扎热叶·艾库拉贺春钰王义海司马义力·买买提尼亚孜木克代斯·拜克提亚尔朱相露韩有溪曹茜
Owner CANCER HOSPITAL AFFILIATED TO HOSPITAL XINJIANG MEDICAL UNIV
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