Prefabricated test powder for rapidly preparing nucleic acid electrophoretic buffer solution based on taurine and use method thereof

Abstract

The invention belongs to the technical field of biological analysis, and provides prefabricated test powder for rapidly preparing a nucleic acid electrophoretic buffer solution based on taurine and ause method thereof. The prefabricated test powder comprises the following components by weight: based on preparation of 1 L of the electrophoretic buffer solution with one-time (1*) concentration, each part of the prefabricated test powder contains 9.6-12 g of Tris base, 3.755-7.51 g of taurine, and 0.186-0.744 g of Na2EDTA.2H2O. At room temperature, each part of the test powder is fully mixed andmutually soluble with 1 L of deionized water, 1L of the electrophoretic buffer solution with 1* concentration is obtained, and the pH value of the electrophoretic buffer solution is 8.7-9.2. The prepared prefabricated test powder provided by the invention can rapidly prepare the nucleic acid electrophoretic buffer solution with fixed concentration and fixed pH value. The prepared buffer solutionhas long electrophoretic life and good positive and negative electrode stability. The prepared electrophoretic buffer solution is in favor of protection of DNA and RNA against degrading during electrophoresis, maintains a stable double-helix secondary structure, and enhances the accuracy and sensitivity of fluorescence detection after nucleic acid electrophoresis.

Description

technical field [0001] The invention belongs to the field of biological analysis, and provides a prefabricated test powder for rapid preparation of nucleic acid electrophoresis buffer in biochemical or molecular biological analysis, a preparation method and a use method thereof. Background technique [0002] Electrophoresis detection plays an important role in the biochemical and molecular biological analysis of nucleic acids. It is one of the routine methods for nucleic acid quality and concentration detection, and an important basis for analyzing the size, mass concentration and purity of DNA and RNA fragments. Traditional nucleic acid electrophoresis mainly uses acetate buffer system TAE (Tris-Acetic acid-EDTA) or borate buffer system TBE (Tris-Boric acid-EDTA), and the laboratory needs to prepare the solution independently. Not only the preparation process is time-consuming and labor-intensive, but also the pH value of the solution needs to be adjusted before use after t...

AI Technical Summary

Problems solved by technology

Not only the preparation process is time-consuming and labor-intensive, but also the pH value of the solution needs to be adjusted before use after the components are dissolved, which increases the time and error probability of buffer preparation. At the same time, the electrophoresis life of TAE and TBE is short, at 4-5 hours After the nucleic acid electrophoresis, the pH value of the positive and negative poles of the electrophoresis tank will change significantly, which is not conducive to the stability of the structure of DNA and RNA, and it is necessary to use a new buffer for electrophoresis