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Angiotensin II magnetic particle chemiluminescence detection kit and detection method

A technology of angiotensin and chemiluminescence method, which is applied in the field of magnetic particle chemiluminescence method angiotensin II assay kit, can solve the problems of many operation steps, pollution, and need for further improvement, and achieve high detection sensitivity, improved sensitivity, The effect of good application prospects

Inactive Publication Date: 2018-09-18
TAIZHOU ZECEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the detection methods for detecting angiotensin II are radioimmunoassay technique and chemiluminescence method. Among them, radioimmunoassay technique has the defects of radioactive pollution and many operation steps, while chemiluminescence method has high sensitivity, good specificity, simple operation and environmental protection. Friendly advantages, but chemiluminescence methods include streptavidin magnetic particle chemiluminescence immunoassay technology and fluorescein isothiocyanate magnetic particle chemiluminescence immunoassay technology, both of which have high sensitivity, good specificity and simple operation , environmental friendliness, but the sensitivity and specificity of fluorescein isothiocyanate magnetic particle chemiluminescence immunoassay technology in the detection of angiotensin II still need to be further improved

Method used

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  • Angiotensin II magnetic particle chemiluminescence detection kit and detection method
  • Angiotensin II magnetic particle chemiluminescence detection kit and detection method
  • Angiotensin II magnetic particle chemiluminescence detection kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Angiotensin II Magnetic Particle Chemiluminescence Detection Kit

[0029] The kit of this embodiment includes a magnetic separation reagent for streptavidin magnetic particles, a biotinylated angiotensin II monoclonal antibody, an alkaline phosphatase-labeled angiotensin II derivative, and a calibrator.

[0030] The angiotensin II antibody used in this example is a monoclonal antibody, the antibody is a mouse antibody, and the calibrator and sample contain the antigen to be tested.

[0031] 1. The preparation steps of biotinylated angiotensin II monoclonal antibody are as follows:

[0032] 1) Measure 1 mg of angiotensin II monoclonal antibody;

[0033] 2. Calculate based on the molecular weight of angiotensin II monoclonal antibody (30000) and the molecular weight of biotin-NHS (587), and weigh the biotin-NHS according to the molar ratio of 1:20;

[0034] 3) Weigh about 0.4 mg of biotin-NHS, and dissolve it with DMSO to a final concentration of 5 mg / ml;

[...

Embodiment 2

[0063] Comparison of the sensitivity (Table 1), precision (Table 2) and serum test (Table 3) of Example 2 and Comparative Example 1.

[0064] The sensitivity of table 1 embodiment 2 and comparative example 1

[0065]

[0066]

[0067] The precision of table 2 embodiment 2 and comparative example 1

[0068]

[0069] Table 3 embodiment 1 and comparative example 1 measured value and given value contrast and contrast between the measured value

[0070]

[0071]

[0072] It can be seen from the results that the correlation between the measured values ​​and given values ​​of different systems is above 0.9, indicating that the correlation is good, and the correlation between the measured values ​​of different systems is greater than 0.9, indicating that the measured values ​​of system 1 and system 2 are consistent.

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Abstract

The invention discloses an angiotensin II magnetic particle chemiluminescence detection kit, which comprises a streptavidin magnetic particle magnetic separation reagent, a biotinylated angiotensin IImonoclonal antibody, an alkaline phosphatase-labeled angiotensin II derivative and a calibrator. The kit has the advantages of high sensitivity, selectivity and specificity. The invention also discloses a detection method of the angiotensin II magnetic particle chemiluminescence detection kit, and the method is simple, avoids radioactive contamination and prolongs the expiration date of the reagent.

Description

technical field [0001] The invention belongs to the technical field of chemical diagnostic reagents, in particular to a magnetic particle chemiluminescence assay kit and a detection method for angiotensin II. Background technique [0002] Renin produces angiotensin I by cleaving angiotensinogen synthesized by the liver. Angiotensin I can be further cleaved into angiotensin II (Angiotensin II) by angiotensin converting enzyme (AngiotensinConVertingEnzyme, ACE). [0003] Angiotensin II is the most important component of angiotensin. There are angiotensin receptors on the smooth blood vessels of the human body, cells of the zona glomerularis of the adrenal cortex, and cells of some parts of the brain, heart and kidney organs. Angiotensin II binds to angiotensin receptors, causing corresponding physiological effects. Angiotensin acts on vascular smooth muscle, which can constrict arterioles throughout the body and increase arterial blood pressure. Angiotensin II is one of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/535G01N33/543
CPCG01N33/577G01N33/535G01N33/54326
Inventor 李婷婷刘振世
Owner TAIZHOU ZECEN BIOTECH CO LTD
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