Quantum dot immunochromatography detection card of utilizing ternary system immune competition method to detect sildenafil medicines and detection method thereof
A technology of sildenafil and ternary system, which is applied in the direction of measuring devices, material inspection products, analytical materials, etc., can solve the problems of low detection signal strength and sensitivity, inability to uniformly prepare signal substances, insufficient stability, etc., and achieve activity Favorable protective effect, short detection time, and high light energy conversion efficiency
Pending Publication Date: 2018-09-18
SHAOGUAN COLLEGE
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Problems solved by technology
3. HPLC and LC-MS can only detect known sildenafil. For the emerging sildenafil analogues whose molecular structure has been deliberately Phase / tandem ion trap mass spectrometry (LC / ESI-MS / MS) technology can detect
The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive
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[0033] see figure 1 , which is the quantum dot immunochromatographic detection card for the detection of sildenafil drugs by the ternary system immunocompetitive method of this embodiment, including PVC liners, and sequentially adhered to the PVC liners and partially stacked between adjacent ones. A detection antigen release pad, a quantum dot probe release pad, a nitrocellulose membrane (NC membrane) and an absorbent pad are provided; the detection antigen release pad includes a detection antigen coupled by OVA and sildenafil drugs; The quantum dot probe release pad comprises a quantum dot probe marked with an anti-OVA antibody; the nitrocellulose membrane is provided with a detection line and a quality control line, and the detection line is fixed with an anti-sildenafil drug antibody, so The above quality control line was fixed with OVA antigen.
[0034] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane with...
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The invention relates to a quantum dot immunochromatography detection card of utilizing a ternary system immune competition method to detect sildenafil medicines and a detection method thereof. The detection card comprises a liner plate, a detection antigen release pad, a quantum dot probe release pad, a chromatography membrane, and a water absorption pad. The detection antigen release pad comprises a detection antigen, which is formed by coupling ovalbumin and sildenafil medicines. The quantum dot probe release pad comprises a quantum dot probe, which is labeled by an anti-ovalbumin antibody.The chromatography membrane is provided with a detection line and a quality control line. An anti-sildenafil antibody is fixed on the detection line. An ovalbumin antigen is fixed on the quality control line. The detection antigen is dissolved into a detection system from the release pad and forms a ternary system immune complex with the detection antibody and the labeled antibody, and a fluorescence detection signal is generated. Under the detection and reference effects of the quality control line, based on the fluorescence signal of the detection line, people can judge whether a sample contains sildenafil medicines or not, the strength and stability of detection signals are both enhanced, and the detection sensitivity and quantifying precision are improved.
Description
technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health care products and Chinese patent medicines, especially the quantum dots for the detection of sildenafil drugs by the ternary system immune competition method Immunochromatography detection card and detection method. Background technique [0002] In the early 1990s, a PDE-5 inhibitor drug used by Pfizer for the treatment of coronary heart disease was found to be effective in the treatment of impotence in clinical trials. Huge business benefits. [0003] Driven by huge market interests, lawbreakers illegally add sildenafil drugs (including its various structural analogues) to natural impotence products to increase the impotence effect to promote sales and seek illegal benefits. The harm of this kind of behavior to public health has become a worrying global...
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Login to View More IPC IPC(8): G01N33/58
CPCG01N33/582G01N33/9453
Inventor郭杰标钟瑞敏章仕杰钟林涛谢伟光蒋昊黄钻元朱哲
OwnerSHAOGUAN COLLEGE