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A strain of Trichoderma pleurotus and its application in the control of jujube anthracnose

A technology for Trichoderma pleurotus and anthracnose, which is applied in the field of biological control preparations, can solve the problems of harming non-target organisms, destroying ecological balance, and drug resistance, and achieves fast growth, strong stress resistance, and large spore production Effect

Inactive Publication Date: 2020-03-27
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of chemical fungicides has brought about a series of serious problems: pesticide residues, environmental pollution, drug resistance, damage to non-target organisms to destroy ecological balance, etc.
However, it has not been reported that Trichoderma pleurotus is used to control jujube anthracnose

Method used

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  • A strain of Trichoderma pleurotus and its application in the control of jujube anthracnose
  • A strain of Trichoderma pleurotus and its application in the control of jujube anthracnose
  • A strain of Trichoderma pleurotus and its application in the control of jujube anthracnose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and purification of Trichoderma pleurotus strain SDLTr16

[0028] Trichoderma pleurotus strain SDLTr16 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from the river mud of Xiaoqing River, Dongying City, and the separation method is:

[0029] (1) Separation: River mud samples were taken from Xiaoqing River in Dongying City. Weigh 1g of river mud into 100mL sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution was spread on the Bengal Red medium plate, and each gradient was coated in three parallels. After culturing at 30°C for 3 days, microbial colonies of different forms on the Bengal Red medium were picked and streaked on the Bengal Red medium plate. , regularly observe the colony growth. Then, the Trichoderma strains were purified by the plate streaking method, and were numbered and...

Embodiment 2

[0041] 1. Fermentation process of Trichoderma pleurotus strain SDLTr16

[0042] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0043] Solid fermentation medium formula (mass percentage): solid material: 50% corn cob, 10% corn flour, 5% cooked soybean meal, 25% bran, 10% rice husk; inorganic salt solution: potassium dihydrogen phosphate 3.5 %, magnesium sulfate 0.04%, ammonium sulfate 4%, and the remainder is water. The solid-to-liquid ratio is 1:0.7 (mass ratio).

[0044] The formulation of a large amount of fermentation medium is the same as that of solid medium.

[0045] Mass solid fermentation process of Trichoderma pleurotus strain SDLTr16:

[0046] ① Strain seed liquid culture: Pick a small amount of spores of Trichoderma pleurotus strain SDLTr16 from the inclined surface of the test tube, transfer them to PDB liquid medium, and culture them on a shaking table at 28°C for 3 to 5 days. This is the seed liquid, and the bacterial activity...

Embodiment 3

[0052] This example provides related experiments on the control effect of Trichoderma pleurotus strain SDLTr16 wettable powder on winter jujube anthracnose.

[0053] 1) Test location

[0054] The test was carried out in Dongzaoyuan, Xiahe Township, Zhanhua District, Binzhou City, Shandong Province. The garden is located in the old course of the Yellow River and belongs to the coastal saline-alkali land with average fertility. The tested variety Zhanhua Dongzao No. 2 is 5 years old, the planting density is 3×3m, the management level is average, and the occurrence of black spot of jujube trees in previous years is relatively heavy. The diseased fruit rate is about 60%, and the serious plot is more than 80%.

[0055] 2) Test drug

[0056] 200 million live spores / gram of Trichoderma pleurotus SDLTr16 wettable powder (prepared in Example 2), 70% mancozeb WP (product of China·Hebei Shuangji Chemical Co., Ltd., commercially available).

[0057] Control object: winter jujube anthra...

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Abstract

The invention discloses a strain of Trichoderma pleurotus and its application in preventing and treating jujube anthracnose. The strain of the present invention is Trichoderma pleuroticola (Trichoderma pleuroticola) strain SDLTr16, which is isolated from the mud of the Xiaoqing River in Dongying City, and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on October 22, 2012. The number is CGMCC No.6694. Tests show that: the biological control preparation of Trichoderma pleurotus of the present invention has good effects on jujube anthracnose, and the control effect is 76.83-92.40%.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a strain of Trichoderma pleurotus and its application in preventing and treating winter jujube anthracnose, and a biological control preparation prepared by using the Trichoderma pleurotus. Background technique [0002] Dongzao is a unique late-maturing jujube variety in China. It is rich in nutrition, contains Vc, cyclic adenosine monophosphate, cyclic guanosine monophosphate, etc., and has good medical and health care effects on various human diseases. Winter jujube is the main economic fruit tree in the Yellow River Delta Basin, and it is also one of the local leading industries. It is developing rapidly. The planting area in Zhanhua County alone reaches 500,000 mu, with an output of 300 million kg. In recent years, the occurrence of winter jujube diseases has become increasingly serious. Winter jujube anthracnose (winter juju...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 刘慇张淑静牛赡光刘盛芳刘幸红秦绪兵
Owner SHANDONG FOREST SCI RES INST
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