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Novel method for rapidly detecting ascorbic acid

A technology of ascorbic acid and a new method, which is applied in the field of chemical analysis and detection, can solve the problems that the accuracy and specificity of the detection results are greatly affected, the detection of ascorbic acid cannot be selectively detected, and the detection results are affected, etc., achieving high sensitivity, low cost, The effect of simple method

Active Publication Date: 2018-10-12
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many literature reports on the detection method of ascorbic acid, but most of them are based on the reduction of ascorbic acid (Journal of Agricultural & Food Chemistry, 2016, 64 (1): 371. Talanta 165 (2017) 346–350. Nanoscale, 2017, 9( 29):10167-10172.SpectrochimicaActa Part A:Molecular and Biomolecular Spectroscopy 180(2017)204–210.Sensors&Actuators B Chemical,2015,212:214-219.Journal of Luminescence 192(2017)47–55.Biosensors85 210–217. Talanta 162 (2017) 135–142.), there are many reducing substances in food and beverages, so these methods have a great impact on the accuracy and specificity of the test results
In a biological environment, glutathione and dopamine coexisting with ascorbic acid will also affect the test results
Most of the previous methods for the detection of ascorbic acid using manganese dioxide nanomaterials cannot achieve selective detection of the above substances

Method used

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  • Novel method for rapidly detecting ascorbic acid
  • Novel method for rapidly detecting ascorbic acid
  • Novel method for rapidly detecting ascorbic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1mMMKMnO 4 Add to 0.01M MES buffer and dissolve with 10mL of distilled water. Then, the mixture was sonicated for about 30 minutes (color changed to tan). The manganese dioxide material was obtained by centrifugation at 10,000 rpm for 10 minutes. After repeated washing with distilled water five times, the final product was dispersed with 10 mL of distilled water and stored at 4°C. The final concentration of MnO2 nanomaterials was 0.68 mM. Prepare ascorbic acid final concentration of 10 μM. UV-Vis spectrophotometer was used to measure the UV absorption spectra, respectively.

Embodiment 2

[0025] 1 mM OPDA mixed with 0.2 mM MnO2 nanomaterials (a); 0.2 mM MnO2 mixed with 20 μM AA, followed by 1 mMOPDA (b); 1 mM OPDA mixed with 20 μM AA (c); 1 mM OPDA (d); 0.2 mM MnO2 (e); 20 μM AA (f). Measure the fluorescence intensity using a fluorescence spectrophotometer (e.g. figure 1 shown). The excitation wavelength was 360 nm, and the emission wavelengths were 425 nm and 568 nm, respectively.

Embodiment 3

[0027] Fluorescence intensity was measured by 1mM OPDA and different concentrations of MnO2 (0, 0.25, 0.05, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5mM). The excitation wavelength is 360 nm, and the emission wavelength is 568 nm.

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Abstract

The invention discloses a novel method for rapidly detecting ascorbic acid. The novel method is characterized in that a manganese dioxide nano material, o-phenylenediamine and AA are simultaneously mixed, and the detection of AA concentration is realized by detecting of changes of fluorescence intensities of the manganese dioxide nano material and the o-phenylenediamine. The method is very simple,and has strong specificity, good stability and fast detection speed, corresponding kits and test strips can be further developed and researched, and the method can meet the requirements of rapid andaccurate on-site detection, and can be applied to food safety detection, disease detection, bioanalysis, environmental analysis and other fields.

Description

technical field [0001] The invention relates to the field of chemical analysis, in particular to a new method for rapid detection of ascorbic acid (AA), and belongs to the technical field of detection. Background technique [0002] Ascorbic acid (AA), also known as vitamin C, is a common water-soluble vitamin that is abundant in fruits and vegetables. AA is also an antioxidant that is artificially added to foods in the food industry. In addition, AA plays an important role in many processes of human daily life and is vital to human life, so AA is added to food and food preservatives to ensure the quality of food. At the same time, AA is also widely used in many other fields, animal feed, beverages, cosmetics, pharmaceutical preparations, etc. A lack of AA in the human body can cause various diseases, including the common cold, scurvy, mental illness, infertility, and cancer. Unfortunately, as an exogenous chemical, AA cannot be synthesized by the body itself and can only ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/82
CPCG01N21/6428G01N33/82
Inventor 刘亚青吕彦龙王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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