A kind of yellow fluorescent carbon dot and its preparation method and application
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A technology of yellow fluorescence and carbon dots, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., to achieve good solubility and dispersibility, expand the application range, and good detection results
Active Publication Date: 2020-11-10
SHANXI UNIV
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Problems solved by technology
However, it is still challenging to controlly prepare fluorescent carbon dots with specific structures using facile preparation methods and apply them to the detection of targeted molecules.
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Embodiment 1
[0032] Preparation method of carbon dots:
[0033] 1) Take 0.30g of o-phenylenediamine and aminobutyric acid respectively in a glass beaker, add 20mL of deionized water, stir thoroughly and ultrasonically dissolve for 15 minutes;
[0034] 2) Transfer the reaction mixture to a hydrothermal reaction kettle, place it in an oven, and react at 160°C for 8 hours to obtain a brown solution;
[0035] 3) Take out the hydrothermal reaction kettle, cool it naturally, dilute the reaction product 10 times, centrifuge at 13000r / m for 15min, remove insoluble matter to obtain a clear yellow solution, and filter it with a 0.22μm membrane to obtain pure carbon dots aqueous solution;
[0036] 4), freeze-drying the carbon dot aqueous solution to obtain a carbon dot solid.
Embodiment 2
[0038] Fluorescent carbon dots prepared in Example 1 were characterized by fluorescence excitation, emission and ultraviolet absorption spectra (see figure 1 ), for AFM and XPS characterization (see Figure 2-3 ), the fluorescent carbon dots prepared by the present invention emit yellow fluorescence, which are monodisperse amorphous nanoparticles, and the surface contains amino, carboxyl, and hydroxyl groups.
Embodiment 3
[0040] Get the fluorescent carbon dot aqueous solution (0.2mg / mL) 2mL that embodiment 1 prepares and place in the fluorescence cuvette, add the norfloxacin solution that 0.2mL concentration is 0.05,0.10,0.15...0.95,1.0mM respectively, mix uniform, the emission spectrum was scanned in a fluorophotometer (λ ex =420nm,λ em =564nm), according to the relationship between the concentration of norfloxacin and the relative fluorescence intensity change value, calculate the detection range and detection limit of carbon dots to norfloxacin. (See Figure 4 )
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Abstract
The invention discloses a yellow fluorescence carbon dot, a preparation method of the yellow fluorescence carbon dot, and an application of the yellow fluorescence carbon dot to label-free detection of quinolone antibiotics. The carbon dot is a yellow fluorescence carbon dot taking o-phenylenediamine and aminobutyric acid as the raw materials and prepared through a one-step hydro-thermal synthesismethod. The preparation method of the carbon dot is simple and convenient, and the prepared carbon dot is stable in optical property and good in water solubility and dispersity. The carbon dot, as afluorescence probe, is used to rapidly sensitively detect the quinolone antibiotics without label due to the special surface structure. When the quinolone antibiotics exist, the fluorescence of the carbon dot is effectively quenched by the quinolone antibiotics, and the content of the quinolone antibiotics in a sample is detected according to the variation degree of the fluorescence. Compared withthe traditional method, the provided quinolone antibiotics detection method is simple, convenient and fast, no label is extraordinarily modified, the sample is directly detected, and the method has more advantages in the practical application.
Description
technical field [0001] The invention relates to a fluorescent carbon point, in particular to a yellow fluorescent carbon point and a preparation method thereof, and the carbon point is used for label-free detection of quinolone antibiotics. Background technique [0002] Quinolones antibiotics are artificially synthesized third-generation antibiotic drugs containing 4-quinolone basic structure for the prevention and treatment of pathogenic bacterial infections. Quinolones target bacterial deoxyribonucleic acid (DNA), hinder DNA gyrase, further cause irreversible damage to bacterial DNA, destroy normal metabolism and reproduction of bacteria, and thus achieve antibacterial effects. Due to its strong penetration, long half-life, and broad antimicrobial activity against Gram-positive and negative bacteria in the environment and food, it is widely used in humans and animals to treat respiratory and skin infectious diseases Wait. However, due to its increasing consumption, it ha...
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