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Preparation method of litsea cubeba fruit essential oil with antibacterial activity

A technology of antibacterial activity and Radix chinensis, applied in the field of plant essential oils, can solve the problems of complicated preparation steps, long preparation time, low extraction rate, etc., and achieve the effects of improving extraction efficiency, shortening extraction time, and excellent bacteriostatic effect.

Active Publication Date: 2018-10-19
清远市瑶康生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chinese patent application CN104120036A discloses a processing method of litsea cubeba oil, which uses steam distillation to obtain litsea cubeba oil through five steps of raw material crushing, distillation, fractionation, condensation, and oil-water separation, and the method shortens the processing time , reduce energy consumption and increase oil yield, but this method has certain requirements for equipment, the preparation time is longer, and the preparation steps are more cumbersome, which is not conducive to popularization of production
Another Chinese patent application CN102552403B discloses a litsea cubeba extract and its anticancer application. The litsea cubeba extract is obtained by drying, steaming, and pressing, which has good anticancer effects. effect, can be used to prepare various anticancer drugs, but this method has a large loss in the extraction process, which makes the required quantity of Litsea cubeba raw materials large, resulting in an increase in cost, and the extraction rate is low, and the quality of the obtained extract poor disadvantage
The existing extraction method takes a long time, so the time cost is relatively large, and it cannot be popularized and applied more effectively

Method used

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  • Preparation method of litsea cubeba fruit essential oil with antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A preparation method of litsea cubeba essential oil with antibacterial activity, comprising the following steps:

[0028] (1) Processing of Litsea Cubeba Raw Material: Put 40 g of Litsea Cubeba after cleaning into a retort.

[0029] (2) Extraction of Litsea Cubeba Essential Oil: Take Litsea Cubeba obtained in step (1) as raw material, add 750 mL of distilled water, extract for 15 min under the condition of microwave power of 750 W, and stand still to take out the upper layer oil sample.

[0030] (3) Drying of Litsea Cubeba Essential Oil: The oil sample obtained in step (2) was dried with anhydrous sodium sulfate to obtain 2.81 g of Litsea Cubeba Essential Oil, with a yield of 7.03%.

Embodiment 2

[0032] A preparation method of litsea cubeba essential oil with antibacterial activity, comprising the following steps:

[0033] (1) Processing of Litsea Cubeba Raw Materials: Litsea cubeba fruit is optimized and washed, and 30 g of the pretreated Litsea Cubeba fruit is weighed and put into a retort.

[0034] (2) Extraction of Litsea Cubeba essential oil: take Litsea cubeba obtained in step (1) as raw material, add 800mL of distilled water, extract for 10min under the condition of microwave power of 800W, and stand still to take out the upper layer oil sample.

[0035] (3) Drying of Litsea Cubeba Essential Oil: The oil sample obtained in step (2) was dried with anhydrous sodium sulfate to obtain 2.09 g of Litsea Cubeba Essential Oil, with a yield of 6.97%.

experiment example 1

[0045] Antibacterial experiment of experimental example 1 litsea cubeba essential oil

[0046] The size of the inhibition zone to Escherichia coli, Aspergillus niger, and Saccharomyces cerevisiae was measured by the filter paper method, and the specific experimental method was as follows:

[0047] (1) Prepare a number of circular filter paper sheets with a diameter of 9mm, divide them into three groups according to the experimental group, positive control group and blank control group, put Litsea cubeba essential oil into them respectively after dry heat sterilization, and use supercritical CO 2 Soak the extracted litsea cubeba essential oil and distilled water for 2 hours;

[0048](2) The concentration of the bacterial cell suspension is 107-108cfu / mL, and the petri dish is divided into Escherichia coli group, Aspergillus niger group and Saccharomyces cerevisiae group, and 0.2mL of bacterial cell suspension is added to each petri dish, and the coating is uniform;

[0049] (3...

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Abstract

The invention provides a preparation method of litsea cubeba fruit essential oil with antibacterial activity. The litsea cubeba fruit essential oil with antibacterial activity mainly comprises 56.60%of citral, 22.95% of Limonene, 7.95% of pinene, 2.81% of laurene, and 1.44% of linalool. According to the preparation method, microwave assisted extraction technology is adopted, an extracted productcan be obtained through destroying gland cells in litsea cubeba fruit skin tissues. In the preparation method, air drying, crushing, and sieving of the litsea cubeba fruit raw material are not needed,essential oil loss can be avoided as far as possible, and at the same time, cost is reduced; and in addition, the microwave assisted extraction technology, so that extraction efficiency is increasedgreatly, and cost is reduced.

Description

technical field [0001] The invention belongs to the field of plant essential oils, in particular to a preparation method of litsea cubeba essential oil with antibacterial activity. Background technique [0002] Litsea cubeba, also known as Bicheng eggplant, pheasant pepper, wood ginger, etc., is a deciduous shrub or small tree of the genus Zingia lauraceae, mainly distributed in Hunan, Hubei, Jiangxi, Fujian, Yunnan, and south of the Yangtze River. Guizhou, Zhejiang, Anhui, Guangdong and other provinces. Litsea Cubeba is an important aromatic plant in my country, and its peel, leaves and flowers contain volatile oil—Litsea cubeba essential oil. [0003] Litsea cubeba essential oil refers to the natural essential oil extracted from the fresh fruit of Litsea cubeba. It is a light yellow to orange oily liquid, and its main components are terpenoids, including monoterpenoids and sesquiterpenoids. , in addition to some other compounds. Litsea cubeba essential oil contains a la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02
CPCC11B9/02C11B9/025
Inventor 李颖庄晓慈汪勇何佳静
Owner 清远市瑶康生物科技有限公司
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