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AMLI-ETO fused gene detection kit

A detection kit and gene fusion technology, applied in the field of molecular biology, can solve the problems of increasing the stability of the solution, reducing the requirements, etc., and achieve the effects of accurate and reliable detection results, improved storage resistance, and avoidance of false negative results.

Active Publication Date: 2018-10-23
SUREXAM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is an urgent need to develop a premixed fluorescent probe working solution with added probe stabilizer, which can be stored without degradation or side reactions to simplify the required operations, greatly reduce the requirements for users and the use environment, and provide Convenience, but at the same time, higher requirements are put forward for the stability of the kit, which increases the difficulty of ensuring the stability of its solution. In view of this, the invention

Method used

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  • AMLI-ETO fused gene detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0032] An embodiment of the gene probe stabilizer of the present invention, the gene probe stabilizer comprises the following components: 7.8g / L sodium citrate, 14.6g / L NaCl, 110g / L dextran sulfate, the volume percentage is 72% deionized formamide, 3.8mmol / L Tris-HCl, 0.5mmol / L EDTA, 20mmol / L betaine and 0.1% by volume of polyvinylpyrrolidone.

Embodiment 2

[0034] An embodiment of the gene probe stabilizer of the present invention, the gene probe stabilizer comprises the following components: 8g / L sodium citrate, 12g / L NaCl, 90g / L dextran sulfate, volume percentage is 45% Deionized formamide, 3mmol / L Tris-HCl, 0.8mmol / L EDTA, 15mmol / L betaine and 0.2% polyvinylpyrrolidone by volume.

Embodiment 3

[0036] An embodiment of the gene probe stabilizer of the present invention, the gene probe stabilizer comprises the following components: 6g / L sodium citrate, 15g / L NaCl, 150g / L dextran sulfate, volume percentage is 80% Deionized formamide, 8mmol / L Tris-HCl, 1mmol / L EDTA, 25mmol / L betaine and 0.3% by volume polyvinylpyrrolidone.

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Abstract

The invention discloses a gene probe stabilizing agent. The gene probe stabilizing agent is prepared from the following components: sodium citrate, NaCl, dextran sulfate, deionized formamide, Tris-HCl, EDTA (Ethylene Diamine Tetraacetic Acid), betaine and polyvinyl pyrrolidone. The invention also discloses application of the gene probe stabilizing agent and an AMLI-ETO fused gene detection kit containing the gene probe stabilizing agent. According to the gene probe stabilizing agent provided by the invention, degradation of a probe in a storage process can be effectively prevented, a retentionperiod of the gene probe stabilizing agent is prolonged, the storage resistance of the kit is improved, the reliability of a detection result can be ensured, and a false negative result is avoided. The AMLI-ETO fused gene detection kit containing the gene probe stabilizing agent disclosed by the invention has the advantages of good stability, high sensitivity and specificity, and accurate and reliable detection result.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a gene probe stabilizer and an AML1-ETO fusion gene detection kit containing the probe stabilizer. Background technique [0002] AML1-ETO fusion gene is also a diagnostic indicator of AML, and it is also a prognostic indicator of good prognosis. Acute myeloid leukemia (acute myeloid leukemia, AML) is mainly manifested as the malignant proliferation of myeloid blasts. It has two subtypes: undifferentiated myeloid leukemia (M1) and partially differentiated myeloid leukemia (M2). It is reported that t(8;21)(q22;q22) chromosomal translocation exists in l2%-20% of acute myeloid leukemia (AML), especially in AML-M2, the incidence rate can be as high as 40%-80%. [0003] The AML1 gene located on chromosome 21q22 is fused with the ETO gene of 8q22 to produce AML1-ETO and ETO-AML1 fusion genes. It is generally believed that the expression level is extremely low or t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886
Inventor 许嘉森吴诗扬刘志明朱蓉彭璨璨
Owner SUREXAM BIO TECH
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