Strain with antagonistic action on cabbage sclerotinia sclerotiorum as well as culture method and application of strain

Abstract

The invention discloses a strain with an antagonistic action on cabbage sclerotinia sclerotiorum. The strain is paenibacillus kribbensis and the diameter of a single thallus is (0.5 to 0.7)*(2 to 3) microns; the strain has no bacterial capsule and peripheral flagellum. The strain is gram positive and a spore has the size of (0.7 to 0.9)*(1.1 to 1.4) microns and has an oval shape. A bacterial colony has a rough and non-transparent surface and is white or slightly yellow; after the bacterial colony is subjected to bacterium shake culture in a potato dextrose liquid culture medium for 72h, the bacterial colony is slightly yellow; the strain is preserved in China Center for Type Culture Collection (CCTCC) on April 26, 2018, with the preservation number of CCTCC M 2018236. The strain provided by the invention is simple to produce and low in cost, and has no toxicity, no harms and no environment pollution; the growth of cabbage sclerotinia sclerotiorum hyphae is safely and effectively inhibited; meanwhile, green cultivation of cabbages is facilitated and a farmland ecosystem is protected.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a bacterial strain with antagonistic effect on Sclerotinia sclerotiorum of Chinese cabbage and its cultivation method and application. Background technique [0002] Bacteria of the genus Paenibacillus have been isolated from a variety of environments, and many species are associated with humans, animals, plants, and the environment. Most of the bacteria of the genus Paenibacillus are found in the soil, and are usually plant root growth-promoting bacteria. These bacteria of the genus Paenibacillus can promote plant growth and can be used in agricultural production. Some Paenibacillus bacteria produce antibacterial compounds that are useful in medicine or pesticides, and others are used to produce enzymes for bioremediation or to produce valuable chemicals. Paenibacillus polymyxa can produce polypeptide antibiotics, antagonistic proteins, enzymes, plant hormones, flocculants and o...

AI Technical Summary

Problems solved by technology

The excessive use of chemical pesticides in traditional agriculture will not only increase the amount of pesticide residues, but also lead to the resistance of many crop diseases and insect pests, and cause pollution to soil, water and ecological environment

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