Strain with antagonistic action on cabbage sclerotinia sclerotiorum as well as culture method and application of strain

A technology of sclerotinia and antagonism, which is applied in the field of strains and their cultivation, can solve the problems of soil, water and ecological environment pollution, increase of pesticide residues, etc., and achieve no environmental pollution, safe and effective growth, and protection of farmland ecosystems Effect

Inactive Publication Date: 2018-11-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The excessive use of chemical pesticides in traditional agriculture will not only increase the amount of pesticide residues, but also lead to the resistance of many crop diseases and insect pests, and cause pollution to soil, water and ecological environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Strain with antagonistic action on cabbage sclerotinia sclerotiorum as well as culture method and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0011] Effect of Antagonistic Antibacterial Fermentation Broth on Mycelia Growth of Sclerotinia sclerotiorum

[0012] There are three treatments: treatment 1 is control (ck) potato sucrose (PS) liquid medium 300ul; treatment 2 is 150ul fermentation broth + 150ul potato sucrose (PS) liquid medium; treatment 3 is 300ul fermentation broth. Every treatment is repeated 3 times, respectively coated on the PSA medium, and then use a 5mml puncher to get the bacterium cake at the edge of the colony of Sclerotinia sclerotiorum, and then inoculate it on the PSA medium that has been coated In the center of the plate, after culturing at 28°C for 72 hours, m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a strain with an antagonistic action on cabbage sclerotinia sclerotiorum. The strain is paenibacillus kribbensis and the diameter of a single thallus is (0.5 to 0.7)*(2 to 3) microns; the strain has no bacterial capsule and peripheral flagellum. The strain is gram positive and a spore has the size of (0.7 to 0.9)*(1.1 to 1.4) microns and has an oval shape. A bacterial colony has a rough and non-transparent surface and is white or slightly yellow; after the bacterial colony is subjected to bacterium shake culture in a potato dextrose liquid culture medium for 72h, the bacterial colony is slightly yellow; the strain is preserved in China Center for Type Culture Collection (CCTCC) on April 26, 2018, with the preservation number of CCTCC M 2018236. The strain provided by the invention is simple to produce and low in cost, and has no toxicity, no harms and no environment pollution; the growth of cabbage sclerotinia sclerotiorum hyphae is safely and effectively inhibited; meanwhile, green cultivation of cabbages is facilitated and a farmland ecosystem is protected.

Description

technical field [0001] The invention relates to the field of bacterial strains, in particular to a bacterial strain with antagonistic effect on Sclerotinia sclerotiorum of Chinese cabbage and its cultivation method and application. Background technique [0002] Bacteria of the genus Paenibacillus have been isolated from a variety of environments, and many species are associated with humans, animals, plants, and the environment. Most of the bacteria of the genus Paenibacillus are found in the soil, and are usually plant root growth-promoting bacteria. These bacteria of the genus Paenibacillus can promote plant growth and can be used in agricultural production. Some Paenibacillus bacteria produce antibacterial compounds that are useful in medicine or pesticides, and others are used to produce enzymes for bioremediation or to produce valuable chemicals. Paenibacillus polymyxa can produce polypeptide antibiotics, antagonistic proteins, enzymes, plant hormones, flocculants and o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 雷娟利钟新民李必元岳智臣陶鹏赵彦婷
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap