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A kind of compound screening and identification method of soybean salt tolerance

An identification method and a technology of salt tolerance, applied in the agricultural field, can solve problems that have not been reported, and achieve the effects of strong operability, clear material components, and simple and efficient screening indicators

Active Publication Date: 2020-09-04
JIANGSU ACAD OF AGRI SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

Large-scale indoor screening and identification of crops are mostly carried out at the germination and seedling stages, but there is no report on the combined screening and identification of soybean salt tolerance at the germination and seedling stages

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this laboratory, the soybean variety Nannong 1138 (approved variety, commercially available) was used as the mutagenic receptor material, and about 2000 Nannong 1138 soybean mutant libraries were constructed through EMS chemical induction and Co60 radiation methods. According to the above method, the mutant materials were screened for salt tolerance, and a series of materials with different salt tolerance were obtained. The specific implementation methods are as follows:

[0037] 1. Screening concentration pre-test:

[0038]1. Germination period: Under the conditions of the glass greenhouse of our research laboratory, a plastic cup with an upper diameter of 8 cm, a lower diameter of 6 cm, and a depth of 11 cm was selected for sowing, and three small holes were drilled at the bottom of the cup as channels for the solution to circulate. In order to keep each sowing cup and the total dry weight of the contents basically the same, each cup is first filled with dry vermic...

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Abstract

The invention discloses a soybean-salt-resistance composite screening and identification method. In the method, the germination period and the seedling period are combined, the soybean materials are subjected to salt-resistance screening and identification with the continuous multi-generation-continuous-screening composite strategy, and the salt resistance of different soybean germplasm resourcesis comprehensively assessment evaluated. According to the soybean-salt-resistance composite screening and identification method, vermiculite is used as a fixed matrix, the saline concentration is accurately controlled with the water level keeping method, the emergence rate, the plant height and the growth state serve as main salt-resistance indexes, a series of different materials which have saltresistance and can stably inheriting through the strict controllable screening program are obtained, and a material basis is laid for soybean excellent species cultivation and salt-resistance mechanism studying. The soybean-salt-resistance composite screening and identification method is efficient, reliable, simple, practical and high in operability, and has high production realistic significance.

Description

technical field [0001] The invention relates to a compound screening and identification method of soybean salt tolerance, which belongs to the field of agricultural technology. Background technique [0002] Saline soil mainly includes saline-alkali land in inland arid and semi-arid areas and coastal areas with high salinity tidal flats, which are widely distributed in the world, with an area of ​​about 1 billion hectares (Wang Zunqin, 1993). There are also about 90 million hectares of saline soil (Li Bin et al., 2005) in the mainland and eastern coastal areas, accounting for up to 20% of the country's cultivated land. Salinization is becoming more and more serious, coupled with the continuous silting of coastal beaches, the area of ​​salinized soil is still expanding. Large-scale soil salinization has caused a large number of low-yield fields, which has had a serious impact on agricultural production and the agricultural ecological environment. The comprehensive agricultura...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/40A01G24/15A01H1/04
CPCA01G22/40A01G24/15A01H1/04
Inventor 黄益洪邵宏波徐照龙戚维聪陆海鹰刘佳
Owner JIANGSU ACAD OF AGRI SCI