Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sogatalla furcifera UDP-N-acetylglucosamine pyrophosphorylase gene segment and application of its dsRNA

A technology of UDP-N- and acetylglucosamine pyrolysis, applied in the field of insect genetic engineering

Inactive Publication Date: 2018-11-06
KAILI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since chitin does not exist in humans and other higher animals, the chitin synthesis pathway is relatively safe as a target for pest control, but there is no research on UDP-N-acetylglucosamine pyrophosphorylase in white-backed planthopper. Related reports, there is no report on the dsRNA targeting the UDP-N-acetylglucosamine pyrophosphorylase gene of white-backed planthopper

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sogatalla furcifera UDP-N-acetylglucosamine pyrophosphorylase gene segment and application of its dsRNA
  • Sogatalla furcifera UDP-N-acetylglucosamine pyrophosphorylase gene segment and application of its dsRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The nucleotide sequence of UDP-N-acetylglucosamine pyrophosphorylase gene of white-backed planthopper is shown as SEQ ID NO: 3; the nucleotide sequence is a fragment obtained based on the transcriptome database of white-backed planthopper, and the design specificity Primers were obtained by PCR amplification. The nucleotide sequence is 1470bp in length. The amino acid sequence encoded by the UDP-N-acetylglucosamine pyrophosphorylase gene of white-backed planthopper is as SEQ ID NO: 4; the amino acid sequence is obtained by using ExPASy software to translate the nucleotide sequence SEQ ID NO: 3 into amino acids and analyze their protein properties. The results showed that the UDP-N-acetylglucosamine pyrophosphorylase gene encoded 489 amino acids, the relative molecular weight was 55.07kDa, and the theoretical isoelectric point was 6.37.

[0021] White-backed planthopper UDP-N-acetylglucosamine pyrophosphorylase gene fragment and the method for obtaining the gene fragme...

Embodiment 2

[0023] Example 2: Sequence of UDP-N-acetylglucosamine pyrophosphorylase gene of white-backed planthopper and its encoded amino acid

[0024] 1. Acquisition of full-length sequence of UDP-N-acetylglucosamine pyrophosphorylase gene of white-backed planthopper

[0025] 1) Preliminary analysis of the transcriptome database of the white-backed planthopper was carried out using bioinformatics technology, and two fragments of the SfUAP gene of the white-backed planthopper were determined and obtained through programs such as NCBI database Blast. The following specific primers were designed using Primer Premier6.0 software: upstream primer SfUAP-F 5'-GAACGAGAGGAACTGTGT-3', downstream primer SfUAP-R 5'-GTTGGTGACGACTTCTGT-3', all primers were provided by Sangon Bioengineering (Shanghai) Co., Ltd. Synthetic.

[0026] 2) Select 15 5th-instar nymphs of white-backed planthopper, place them in a 1.5mL RNase-free centrifuge tube, freeze them in liquid nitrogen, grind them into fine powder qu...

Embodiment 3

[0034] Example 3: Synthesis of dsRNA of the white-backed planthopper UDP-N-acetylglucosamine pyrophosphorylase gene

[0035] 1) Based on the obtained SfUAP gene full-length sequence SEQ ID NO: 3, use Primer Premier 6.0 software to design upstream primer 2 SEQ ID NO: 5 and downstream primer 2 SEQ ID NO: 6 with T7 promoter, all primers were produced by Synthesized by Industrial Bioengineering (Shanghai) Co., Ltd.

[0036] 2) Using the cDNA synthesized in Example 2 as a template, PCR amplification was performed using the dsRNA primers synthesized above. The total volume of the PCR reaction system is 25 μL, including: 1 μL each of upstream and downstream primers (10 μM), 10×LA PCR Buffer (Mg 2+ plus) 2.5 μL, dNTP Mixture (2.5mM) 4 μL, cDNA 2 μL, Taq enzyme 0.25 μL and redistilled water 14.25 μL. The PCR reaction conditions were: pre-denaturation at 94°C for 3 min; denaturation at 94°C for 30 s, annealing at 63°C for 30 s, and extension at 72°C for 1 min, a total of 30 cycles; fi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a sogatalla furcifera UDP-N-acetylglucosamine pyrophosphorylase gene segment and application of its dsRNA and belongs to the technical field of insect genetic engineering. Thecandidate gene is provided for sogatalla furcifera RNA mediated pest control strategies, and meanwhile, a theoretical basis is provided for research and development of novel insecticides.

Description

technical field [0001] The invention relates to the technical field of insect genetic engineering, in particular to the application of a UDP-N-acetylglucosamine pyrophosphorylase gene fragment of white-backed planthopper and its dsRNA in pest control. Background technique [0002] The white-backed planthopper Sogatalla furcifera (Horváth) belongs to the Hemiptera planthopper family Delphacidae and is an important pest in rice production in the Asia-Pacific region. It mainly directly sucks the juice of rice plants through adults and nymphs, causing slow growth of rice, delayed tillering, increased shrunken grains, and can cause rice plants to die when seriously damaged; in addition, white-backed planthoppers are also southern rice black-streaked dwarf virus ( Southern rice black-streaked dwarf virus, SRBSDV) is the main medium of transmission, and the harm it causes is even more devastating. At present, the prevention and control of white-backed planthopper still mainly depe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/10C12N9/12C12N15/113A01N57/16A01P7/04
CPCA01N57/16C12N9/1241C12N15/113
Inventor 王召杨洪朱环金道超周操龙贵云
Owner KAILI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com