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Bacterial-derived anti-tumor PNPase gene as well as preparation method and application thereof

An anti-tumor and genetic technology, applied in the field of anti-tumor, can solve problems such as unsatisfactory effects, and achieve the effect of inhibiting proliferation and invasion

Inactive Publication Date: 2021-06-25
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, on a global scale, the main methods of tumor treatment include surgical resection, radiotherapy and chemotherapy, and immunotherapy, etc., but the effects of these methods are not satisfactory. Searching for new effective treatment methods and methods has become an urgent task in the current clinical treatment of tumors.

Method used

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  • Bacterial-derived anti-tumor PNPase gene as well as preparation method and application thereof
  • Bacterial-derived anti-tumor PNPase gene as well as preparation method and application thereof
  • Bacterial-derived anti-tumor PNPase gene as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: A bacterial-derived anti-tumor PNPase gene, the anti-tumor PNPase gene is a nucleotide fragment of the SV40 nuclear localization signal sequence added to the 3' end of the PNPase gene sequence, and the DNA sequence of the anti-tumor PNPase gene is as shown in SEQ ID NO.1 Show.

[0019] The protein sequence of the anti-tumor PNPase gene is shown in SEQ ID NO.2.

Embodiment 2

[0020] Example 2: Application of a bacterial-derived anti-tumor PNPase gene, application of the bacterial-derived anti-tumor PNPase gene in inhibiting tumors.

Embodiment 3

[0021] Embodiment 3: A kind of preparation method of the anti-tumor PNPase gene of bacterial origin, this method is: clone the protein coding region of the PNPase gene of E. The oligonucleotide sequence enables the PNPase fusion protein expressed in the cell to be located in the eukaryotic cell, and uses the RNA hydrolysis property of PNPase to directly hydrolyze a variety of RNAs in the nucleus, blocking RNA protein translation and exerting various physiological functions, thereby Inhibit the growth, proliferation and invasion of cancer cells.

[0022] 1. Experimental reagents, materials and instruments

[0023] 1. Experimental reagents and materials

[0024] Blood / cell / tissue genomic DNA extraction kit, G×L DNA Polymerase, MiniBESTAgarose Gel DNA Extraction Kit (Ver.4.0), pMD18-T vector, Xho I and EcoR I, DNAA-Tailing Kit, endotoxin-free plasmid extraction kit, and fluorescent quantitative PCR kit were purchased from TakKaRa Corporation. Newborn bovine serum was purchas...

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Abstract

The invention discloses a bacterial-derived anti-tumor PNPase gene as well as a preparation method and application thereof. The anti-tumor PNPase gene is a nucleotide segment formed by adding an SV40 nuclear localization signal sequence to the 3' terminal of a PNPase gene sequence, and the DNA sequence of the anti-tumor PNPase gene is shown as SEQ ID NO.1. According to the present invention, by using the strong RNA exo-hydrolysis characteristic of the bacteria PNPase, the bacteria PNPase is expressed and positioned in the cancer cell nucleus by using the gene engineering technology so as to directly hydrolyze a variety of RNAs transcribed in the cancer cell nucleus, block RNA protein translation and a variety of physiological functions, and inhibit the proliferation and invasion of cancer cells.

Description

technical field [0001] The invention belongs to the field of anti-tumor technology, and relates to a bacterial-derived anti-tumor PNPase gene and its preparation method and application. Background technique [0002] Cancer treatment is an important topic in today's medical field, and it is also an unavoidable social problem. According to the 2018 "Global Cancer Report", due to Chinese people's bad living habits, population aging, environmental pollution and lack of popularization of early cancer screening For many reasons, the incidence and mortality of cancer in China remain high, and the incidence and mortality of three types of cancer, lung cancer, liver cancer and gastric cancer, account for about half of the world's total, making my country's current medical tasks extremely difficult. At present, on a global scale, the main methods of tumor treatment include surgical resection, radiotherapy and chemotherapy, and immunotherapy, etc., but the effects of these methods are n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/22C12N15/85A61P35/00
CPCC12N9/22C12N15/85A61P35/00
Inventor 孙达权石静徐国强薛殿婷石松余畅何淼
Owner GUIZHOU MEDICAL UNIV
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