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A SNP site significantly related to the precocious traits of Chinese mitten crab and its application

A Chinese mitten crab, early-maturing technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as feed waste and affecting the efficiency of river crab breeding

Active Publication Date: 2021-07-02
FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, due to the existence of a large number of precocious first-year-old crabs, a huge waste of feed was also caused during the breeding stage of crab species, which seriously affected the benefits of river crab culture.

Method used

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  • A SNP site significantly related to the precocious traits of Chinese mitten crab and its application
  • A SNP site significantly related to the precocious traits of Chinese mitten crab and its application
  • A SNP site significantly related to the precocious traits of Chinese mitten crab and its application

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Embodiment 1

[0021] Example 1 SNP screening of the 5'-flanking region sequence of the Vg gene and its correlation analysis with precocious puberty:

[0022] ①Preliminary screening of precocious puberty related SNP loci

[0023] 100 samples of normal and precocious crabs were randomly selected for genomic DNA extraction. Design primers to carry out PCR amplification to the 5'-flank region sequence of Vg gene, amplification primer is: Vg1F:ACTCTCGTCTATTTCCACTATC (SEQ ID NO.3); Vg1R:CCATCCTGCGACAATCAC (SEQ ID NO.4); Amplification length is 801bp, for The amplified PCR product was purified and bidirectionally sequenced, and the SNP site of the 5'-flanking regulatory region sequence of the Vg gene was screened and identified according to the sequencing results.

[0024] For the SNP sites found by sequencing, the trait-genotype association analysis of single loci (Logistic regression analysis based on the co-dominance model and additive model) was performed on the precocious and normal developm...

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Abstract

The invention discloses a SNP site significantly related to the precocious trait of Chinese mitten crab and its application. The SNP site is located at the 5'-flanking region-437bp of the Vg gene of the Chinese mitten crab, and the base variation information is SNP g.-437T>G. The PCR primers used to detect the SNP site are SEQ ID NO.1 and SEQ ID NO.2, and the restriction endonuclease is MboI. There is a significant correlation between this SNP site and the precocious puberty of Eriocheir sinensis, and the risk of precocious puberty in individuals of GG genotype is significantly lower than that of individuals with TT genotype. The SNP sites and PCR primers for genotyping are used in predicting the risk of precocious maturation of Chinese mitten crabs and / or in breeding of Chinese mitten crabs.

Description

technical field [0001] The invention belongs to the field of aquaculture genetic engineering, and relates to a SNP site significantly related to the precocious trait of Chinese mitten crab and its application. Background technique [0002] Chinese mitten crab (Eriocheir sinensis), commonly known as river crab, is one of the most important freshwater aquaculture species in my country. Precocious puberty is one of the bottleneck problems that affect the current efficiency of river crab breeding. During the breeding process, the gonads of young precocious crabs mature in the same year, and cannot continue to moult smoothly, and their weight growth is slow or even stagnant. Most of them die during April to May of the following year. , can no longer continue to raise to the size of 2-year-old adult crabs. In addition, due to the existence of a large number of precocious first-year-old crabs, a huge waste of feed was also caused during the breeding stage of crab species, which se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 徐宇许志强林海邓燕飞李旭光陆全平潘建林
Owner FRESHWATER FISHERIES RES INSITUTE OF JIANGSUPROVINCE