A preparation method of trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence

A nano-technology of trans-anethole and albumin, which is applied in the direction of nano-medicine, nanotechnology, nanotechnology, etc., can solve the problems of nano-particle research reports without trans-anethole, increase the drug loading capacity, and simplify the synthesis process , the effect of particle size reduction

Active Publication Date: 2021-05-04
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no research report on nanoparticles of trans-anethole

Method used

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  • A preparation method of trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence
  • A preparation method of trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence
  • A preparation method of trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: take 2 ml of 10 mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonication.

[0040] 2) Slowly add 15 μl of 40 mg / ml trans-anethole solution prepared in dichloromethane and 2 μl of 80 mg / ml indocyanine green solution with a pipette gun.

[0041] 3) After stirring evenly, quickly add 100 μl of glutaraldehyde crosslinking agent aqueous solution, and finally stir and react at 700 rpm / min at 20° C. for 3 hours.

[0042] 4) After the reaction is completed, filter with a 220nm water filter.

[0043] 5) The filtrate was centrifuged at 8,000 rpm / min for 4 minutes, and washed 5 times with deionized water to obtain bovine serum albumin nanoparticles loaded with trans-anethole and indocyanine green.

[0044] The morphology of trans-anethole-albumin nanoparticles prepared with indocyanine green fluorescent tracer was observed by transmission electron microscope ( Picture 1-1 ).

Embodiment 2

[0046] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: take 2 ml of 15 mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonication.

[0047] 2) Slowly add 12 μl of 40 mg / ml trans-anethole solution prepared in dichloromethane and 1 μl of 80 mg / ml indocyanine green solution with a pipette gun.

[0048] 3) After stirring evenly, quickly add 80 μl of glutaraldehyde crosslinking agent aqueous solution, and finally stir and react at 800 rpm / min at 20° C. for 4 hours.

[0049]4) After the reaction is completed, filter with a 220nm water filter.

[0050] 5) The filtrate was centrifuged at 8,000 rpm / min for 4 minutes, and washed 5 times with deionized water to obtain bovine serum albumin nanoparticles loaded with trans-anethole and indocyanine green.

[0051] The morphology of trans-anethole-albumin nanoparticles prepared with indocyanine green fluorescent tracer was observed by transmission electron microscope ( Figure 1-2 ).

Embodiment 3

[0053] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: take 2 ml of 15 mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonication.

[0054] 2) Slowly add 12 μl of a 50 mg / ml trans-anethole solution prepared in dimethyl sulfoxide and 1 μl of a 90 mg / ml indocyanine green solution with a pipette gun.

[0055] 3) After stirring evenly, quickly add 100 μl of glutaraldehyde crosslinking agent aqueous solution, and finally stir and react at 700 rpm / min at 20° C. for 3 hours.

[0056] 4) After the reaction is completed, filter with a 220nm water filter.

[0057] 5) The filtrate was centrifuged at 7,000 rpm / min for 5 minutes, and washed 6 times with deionized water to obtain bovine serum albumin nanoparticles loaded with trans-anethole and indocyanine green.

[0058] The morphology of trans-anethole-albumin nanoparticles prepared with indocyanine green fluorescent tracer was observed by transmission electron microscope ( Figure 1-3 ).

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Abstract

The invention relates to a method for preparing trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence; using albumin as a raw material, adjusting the concentration of indocyanine green fluorescent dye and trans-anethole drug Ratio and solvent, to fully control the size of the particle size, to prepare particles with a particle size of 20-50 nanometers; to simultaneously entrap indocyanine green fluorescent dye and trans-anethole in albumin nanoparticles, so that it has both therapy and imaging capabilities. The drug release in vitro was determined by high performance liquid chromatography. In phosphate buffer solution, the release rate of the sustained-release 96h only reached 31%, which has obvious sustained-release effect. The synthesis process of the invention is simple, non-toxic, fast, and the output is large, and the particle size of the particles is obviously reduced compared with the prior art. In the sustained-release test in vitro, the indocyanine green fluorescently traced trans-anethole-albumin nanoparticles have a good sustained-release effect, and the release rate only reaches 31% in 96 hours, which has a sustained-release effect.

Description

technical field [0001] The invention relates to the technical field of nanomedicine, in particular to a method for preparing trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence. Background technique [0002] Trans-anethole, the main component of fennel oil, is a natural antioxidant with antibacterial, antifungal and anesthetic properties. Studies have shown that anethole may effectively control some non-immune acute inflammation-related diseases through mechanisms such as inhibiting TNF signaling and inhibiting the production and / or release of PGE 2 and NO, exhibiting anti-inflammatory properties. In acute and persistent inflammation models in mice, it was found that trans-anethole can reduce the concentration of cytokines such as TNF-α; in lipopolysaccharide (LPS)-induced acute lung inflammation model, trans-anethole also showed an Inhibition of total protein concentration, inflammatory cells and inflammatory mediators, etc. [0003] Although t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/14A61K31/085A61K49/00A61K47/42A61P31/04A61P31/10A61P23/00B82Y5/00
CPCA61K9/146A61K31/085A61K49/0034A61K49/0056A61K49/0093A61P23/00A61P31/04A61P31/10B82Y5/00
Inventor 常津杨涵武晓丽高俊潇陈星濛
Owner TIANJIN UNIV
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