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Method for preparing trans-anethole-albumin nanoparticles with indocyanine green fluorescence tracer

A nano-technology of trans-anethole and albumin, which is applied in the directions of nanotechnology, nanotechnology, nano-medicine, etc., can solve the problems such as research reports of nano-particles without trans-anethole, and achieves reduction in particle size, simple and convenient synthesis process, Small particle size effect

Active Publication Date: 2018-11-09
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no research report on nanoparticles of trans-anethole

Method used

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  • Method for preparing trans-anethole-albumin nanoparticles with indocyanine green fluorescence tracer
  • Method for preparing trans-anethole-albumin nanoparticles with indocyanine green fluorescence tracer
  • Method for preparing trans-anethole-albumin nanoparticles with indocyanine green fluorescence tracer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: take 2 ml of 10 mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonication.

[0040] 2) Slowly add 15 μl of 40 mg / ml trans-anethole solution prepared in dichloromethane and 2 μl of 80 mg / ml indocyanine green solution with a pipette gun.

[0041] 3) After stirring evenly, quickly add 100 μl of glutaraldehyde crosslinking agent aqueous solution, and finally stir and react at 700 rpm / min at 20° C. for 3 hours.

[0042] 4) After the reaction is completed, filter with a 220nm water filter.

[0043] 5) The filtrate was centrifuged at 8,000 rpm / min for 4 minutes, and washed 5 times with deionized water to obtain bovine serum albumin nanoparticles loaded with trans-anethole and indocyanine green.

[0044] The morphology of trans-anethole-albumin nanoparticles prepared with indocyanine green fluorescent tracer was observed by transmission electron microscope ( Picture 1-1 ).

Embodiment 2

[0046] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: take 2 ml of 15 mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonication.

[0047] 2) Slowly add 12 μl of 40 mg / ml trans-anethole solution prepared in dichloromethane and 1 μl of 80 mg / ml indocyanine green solution with a pipette gun.

[0048] 3) After stirring evenly, quickly add 80 μl of glutaraldehyde crosslinking agent aqueous solution, and finally stir and react at 800 rpm / min at 20° C. for 4 hours.

[0049]4) After the reaction is completed, filter with a 220nm water filter.

[0050] 5) The filtrate was centrifuged at 8,000 rpm / min for 4 minutes, and washed 5 times with deionized water to obtain bovine serum albumin nanoparticles loaded with trans-anethole and indocyanine green.

[0051] The morphology of trans-anethole-albumin nanoparticles prepared with indocyanine green fluorescent tracer was observed by transmission electron microscope ( Figure 1-2 ).

Embodiment 3

[0053] 1) Preparation of drug-loaded bovine serum albumin nanoparticles: take 2 ml of 15 mg / ml bovine serum albumin aqueous solution, and make it uniform by ultrasonication.

[0054] 2) Slowly add 12 μl of a 50 mg / ml trans-anethole solution prepared in dimethyl sulfoxide and 1 μl of a 90 mg / ml indocyanine green solution with a pipette gun.

[0055] 3) After stirring evenly, quickly add 100 μl of glutaraldehyde crosslinking agent aqueous solution, and finally stir and react at 700 rpm / min at 20° C. for 3 hours.

[0056] 4) After the reaction is completed, filter with a 220nm water filter.

[0057] 5) The filtrate was centrifuged at 7,000 rpm / min for 5 minutes, and washed 6 times with deionized water to obtain bovine serum albumin nanoparticles loaded with trans-anethole and indocyanine green.

[0058] The morphology of trans-anethole-albumin nanoparticles prepared with indocyanine green fluorescent tracer was observed by transmission electron microscope ( Figure 1-3 ).

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Abstract

The invention relates to a method for preparing trans-anethole-albumin nanoparticles with indocyanine green fluorescence tracer. The method comprises the following steps: selecting albumin as a raw material, adjusting the ratio of encapsulated indocyanine green fluorescent dye to trans-anethole drug and solvent, and fully controlling particle size to prepare nanoparticles with size of 20-50 nm; encapsulating the indocyanine green fluorescent dye and trans-anethole simultaneously in albumin nanoparticles, so that the trans-anethole-albumin nanoparticles have therapeutic and imaging functions. The drug release in vitro is determined by high performance liquid chromatography. In phosphate buffer solution, the release rate of sustained release for 96 h is only 31%, and the sustained-released effect is significant. The synthesis process provided by the invention is simple, non-toxic and fast, and has a large yield and significant reduction in the particle size of the particles compared withthe prior art. In an in vitro sustained-release test, the trans-anisole-albumin nanoparticles with indocyanine green fluorescence tracer have good sustained-released effect, and the release rate is only 31% after 96 h, so the nanoparticles have sustained-released effect.

Description

technical field [0001] The invention relates to the technical field of nanomedicine, in particular to a method for preparing trans-anethole-albumin nanoparticles traced with indocyanine green fluorescence. Background technique [0002] Trans-anethole, the main component of fennel oil, is a natural antioxidant with antibacterial, antifungal and anesthetic properties. Studies have shown that anethole may effectively control some non-immune acute inflammation-related diseases through mechanisms such as inhibiting TNF signaling and inhibiting the production and / or release of PGE 2 and NO, exhibiting anti-inflammatory properties. In acute and persistent inflammation models in mice, it was found that trans-anethole can reduce the concentration of cytokines such as TNF-α; in lipopolysaccharide (LPS)-induced acute lung inflammation model, trans-anethole also showed an Inhibition of total protein concentration, inflammatory cells and inflammatory mediators, etc. [0003] Although t...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K31/085A61K49/00A61K47/42A61P31/04A61P31/10A61P23/00B82Y5/00
CPCA61K9/146A61K31/085A61K49/0034A61K49/0056A61K49/0093A61P23/00A61P31/04A61P31/10B82Y5/00
Inventor 常津杨涵武晓丽高俊潇陈星濛
Owner TIANJIN UNIV
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