Application of cuprous oxide nanoparticles for preparing drug for treating bladder cancer

A technology of cuprous oxide and chemotherapeutic drugs, applied in the field of medicine, can solve the problems of unsatisfactory response rate, toxic side effects, and allergic reactions of bladder cancer antitumor drugs, achieve good clinical application prospects, reduce side effects, and reduce recurrence rates

Inactive Publication Date: 2018-11-13
许传亮 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to solve the problems of unsatisfactory response rate, allergic reaction, toxic and side effects, and drug resistance of existing bladder cancer antitumor drugs, and provide a new drug that can treat and / or prevent bladder cancer

Method used

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  • Application of cuprous oxide nanoparticles for preparing drug for treating bladder cancer
  • Application of cuprous oxide nanoparticles for preparing drug for treating bladder cancer
  • Application of cuprous oxide nanoparticles for preparing drug for treating bladder cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: In vitro anti-proliferation experiment of cuprous oxide against bladder cancer cells

[0035] The bladder tumor cell lines 5637 and T24 used in the research were provided by the Urology Laboratory of Changhai Hospital, Second Military Medical University, and were purchased from the Cell Bank of Shanghai Academy of Biological Sciences, Chinese Academy of Sciences.

[0036] (1) Microscopic observation of the cells to be treated: human bladder urothelial cancer cells T24, J82, 5637, SW-780 human normal urothelial cells SVHUC-1; cells in logarithmic growth phase and in good condition were selected and subjected to the above method. Trypsinize, centrifuge to retain the cell pellet, and resuspend into a single cell suspension. After counting by a cell counter, dilute the cell suspension with serum-containing medium to adjust the final cell concentration to 2.5×104 cells / mL.

[0037] (2) Take a 96-well plate, mark it according to the predetermined concentration grad...

Embodiment 2

[0042] Example 2: Cuprous oxide nanoparticles induce cycle arrest in bladder cancer cells

[0043] (1) Select bladder urothelial carcinoma T24 and 5637 cell lines as the research objects, digest, centrifuge and count as above, and dilute the cell concentration to 5×104 cells / mL.

[0044] (2) Take 2mL of cell suspension and add it to each duplicate well of a 6-well plate. The cells are evenly distributed by the figure-eight shaking method. Incubate in a carbon dioxide cell incubator for 6-8 hours until the cells adhere to the wall.

[0045] (3) Observe that the cells in the multiple wells are well adhered to the wall, discard the old medium, and add cuprous oxide nanoparticles-1640 medium with a concentration of 1.25 μg / mL, 2.5 μg / mL, and 5 μg / mL respectively, and add to the blank control group The 1640 medium without cuprous oxide nanoparticles was cultivated in the incubator for 48 hours.

[0046] (4) Digest with trypsin according to the above method and collect the cell pel...

Embodiment 3

[0049] Example 3: Cuprous oxide nanoparticles induce apoptosis of bladder cancer cells

[0050] (1) Select bladder urothelial carcinoma T24 and 5637 cell lines as the research objects, digest, centrifuge and count as above, and dilute the cell concentration to 5×104 cells / mL.

[0051] (2) Pipette 2mL of cell suspension into each duplicate well of the 6-well plate, shake the cells evenly, and incubate in the carbon dioxide cell incubator for 6-8 hours until the cells adhere to the wall.

[0052] (3) Observe that the cells in the multiple wells are well adhered to the wall, discard the old medium, and add cuprous oxide nanoparticles-1640 medium with a concentration of 1.25 μg / mL, 2.5 μg / mL, and 5 μg / mL respectively, and add to the blank control group The 1640 medium without cuprous oxide nanoparticles was cultivated in the incubator for 48 hours.

[0053] (4) Digest with trypsin according to the above method and collect the cell pellets of each duplicate well, add an appropriat...

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Abstract

The invention provides an application of cuprous oxide nanoparticles for preparing a drug for treating and/or preventing bladder cancer. A nano-drug of the cuprous oxide nanoparticles has the good features of a nano material, and shows the remarkable selective anti-tumor effect in an in-vitro experiment. At the same time, the cuprous oxide nanoparticles can be rapidly metabolized in an animal body, and apparent liver and kidney toxicity and other important organ injuries are not discovered, the safety is higher. The application provides new application for a cuprous oxide nanoparticle preparation, and provides a new pharmacy selection for chemotherapy of the bladder cancer. A relapse rate of the bladder tumor is reduced, the side effect of the drug therapy is reduced, and the quality of life of a patient is improved, so the cuprous oxide nanoparticle preparation has a good clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of cuprous oxide nano preparations in the preparation of bladder cancer chemotherapy drugs and bladder cancer infusion therapy drugs. Background technique [0002] Bladder cancer is one of the common malignant tumors of the urinary system. Globally, the incidence of bladder cancer ranks ninth among all malignant tumors. In my country, its incidence ranks sixth among male malignant tumors and first among all genitourinary system malignant tumors. With the advancement of science and technology, bladder cancer diagnosis and treatment techniques are constantly innovating. However, statistics show that the incidence and mortality of bladder cancer in my country have not been significantly improved in the past ten years. [0003] Antineoplastic drug therapy is an important means of treating and preventing bladder cancer in addition to surgery. Bladder infusion therap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/34A61K9/14A61P35/00
CPCA61K9/14A61K33/34A61P35/00
Inventor 许传亮熊巧任乾王野曾蜀雄刘安伟刘飞薛永平
Owner 许传亮
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