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A kind of rapid propagation method of detoxification and microbud grafting of citrus

A citrus, fast technology applied in the field of plant propagation to achieve the effect of simple operation, low cost and easy implementation

Active Publication Date: 2020-06-30
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far, the rapid propagation method using citrus virus-free micro-bud grafting is the first case in China.

Method used

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  • A kind of rapid propagation method of detoxification and microbud grafting of citrus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1. Induction of adventitious buds of citrus: Take the young shoots of tangerine collected from the test field of the College of Horticulture of South China Agricultural University as explants, wash the axillary buds in the lower part of the shoots with tap water, and first use 75% alcohol by volume fraction Scrub and disinfect the surface of the aqueous solution for 10 seconds, wash twice with sterile water, then soak in 0.1% mercuric chloride aqueous solution for 8 minutes, and wash three times with sterile water, and finally cut the stem into 1 cm size and inoculate it in the induction medium The culture conditions were 26-28°C, and the light intensity was 50 μmol m -2 the s -1 , cultivated for 28 days under the condition of light time 8h / d to induce adventitious buds (see figure 1 A); induction medium is: contain 6-benzylaminopurine (BAP) 3.0mg, indole-3-butyric acid (IBA) 0.1mg and 6-furfurylaminopurine (KT) 0.1mg in every liter of medium, The remaining component...

Embodiment 2

[0034] S1. Induction of adventitious buds of citrus: Take the young shoots of tangerine collected from the test field of the College of Horticulture of South China Agricultural University as explants, wash the axillary buds in the lower part of the shoots with tap water, and first use 75% alcohol by volume fraction The surface of the aqueous solution was scrubbed and disinfected for 10 seconds, washed twice with sterile water, then soaked in 0.1% mercuric acid aqueous solution for 8 minutes, washed with sterile water for 3 times, then placed in the refrigerator for 48 hours, and then used with a concentration of 0.1 % mercuric chloride aqueous solution for 2 minutes, rinsed with sterile water for 3 times. Finally, the stems were cut into 1 cm in size and inoculated on the induction medium for cultivation. The cultivation conditions were 26-28°C and the light intensity was 60 μmol m -2 the s -1 Adventitious buds were induced by culturing for 27 days under the condition of ligh...

Embodiment 3

[0040] S1. Induction of adventitious buds of citrus: Take the young shoots of tangerine collected from the test field of the College of Horticulture of South China Agricultural University as explants, wash the axillary buds in the lower part of the shoots with tap water, and first use 75% alcohol by volume fraction Scrub and disinfect the surface of the aqueous solution for 10 seconds, wash twice with sterile water, then soak in 0.1% mercuric chloride aqueous solution for 8 minutes, and wash three times with sterile water, and finally cut the stem into 1 cm size and inoculate it in the induction medium Cultivate on the ground, the culture condition is 26~28℃, the light intensity is 80μmol m -2 the s -1 Adventitious buds were induced by culturing for 28 days under the condition of light time 8h / d; the induction medium was: 3.0mg of 6-benzylaminopurine (BAP) and 0.1mg of indole-3-butyric acid (IBA) per liter of medium and 0.1 mg of 6-furfuryl aminopurine (KT), and the remaining...

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Abstract

The invention discloses a detoxification and gemmule-grafting rapid propagating method for oranges. According to the method, in an entire experimentation process consisting of explant induction, rootstock sterile sowing and grafted seedling obtaining, orange adventitious buds induced are innovatively used as scions and are grafted to aseptic seedlings of rootstocks so as to obtain grafted seedlings, after transplanting, the survival rate reaches up to 93% or more, thus, a large number of non-toxic orange grafted seedlings can be obtained, and the orange industry can be sustainably and healthily developed. The method disclosed by the invention is simple in operation and easy to implement, used culture media and reagents are easy in preparation and low in cost, and thus, the method is proneto large-scale popularization.

Description

Technical field: [0001] The invention belongs to the field of plant propagation, and in particular relates to a rapid propagation method for citrus detoxification and microbud grafting. Background technique: [0002] Citrus retioulata Blanco is an extremely important fruit tree, which is planted in tropical and subtropical regions of the world, and its planting area and total output rank first among fruits. China is one of the important origins of citrus, with rich citrus germplasm resources and a wide variety of fine varieties. Citrus is one of the fruit trees with the largest planting area and output in my country. In 2016, the total planting area of ​​citrus nationwide was 38.412 million mu, and the total output reached 37.649 million tons. The citrus industry has become a supporting industry for fruit and even agriculture in my country, and has had a significant impact on the income of fruit farmers, farmers' employment, and the ecological environment. The development o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/30A01G2/35A01G2/38A01G17/00A01G24/10A01G24/15A01G24/25A01G24/28A01H4/00
CPCA01G2/30A01G2/35A01G2/38A01G17/005A01G24/10A01G24/15A01G24/25A01G24/28A01H4/001A01H4/008
Inventor 陈国华陈冬怡胡群英黄春华黄芳叶秀粦
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI