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Nano-selenium loaded VP1 gene siRNA and preparation method and application thereof

A nano-selenium and gene technology, applied in biochemical equipment and methods, DNA/RNA fragments, genetic engineering, etc., can solve the problems that nano-selenium-carried siRNA anti-virus research has not yet been reported, and achieve enhanced target selectivity and extended residence time Effect of time, long half-life

Inactive Publication Date: 2018-11-20
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the antiviral research of nanoselenium carrying siRNA has not been reported

Method used

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  • Nano-selenium loaded VP1 gene siRNA and preparation method and application thereof
  • Nano-selenium loaded VP1 gene siRNA and preparation method and application thereof
  • Nano-selenium loaded VP1 gene siRNA and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Design and synthesis of siRNA targeting EV71 virus VP1 gene

[0036] The VP1 gene region of the C4 subtype EV71 virus popular in Asia was selected, and siRNA was designed and chemically synthesized.

[0037] The interfering gene site is the 157th nucleotide, and the siRNA sequence is:

[0038] Sense 5'-ggcaucaucaaaugcuagutt-3',

[0039] Antisense 5'-acuagcauuugaugaugcctt-3'.

[0040] The scrambled negative control siRNA (scramble siRNA) sequence is:

[0041] Sense 5'-gcaagaauggugcacccautt-3';

[0042] Antisense 5'-augggugcaccauucuugctt-3'.

[0043] (2) Preparation and characterization of nano-selenium system

[0044]0.1ml of 400μg / ml vitamin C solution was added dropwise to 4ml of 400μg / ml sodium selenite solution, magnetically stirred for 2 hours, and purified nano-selenium solution was obtained after dialysis. Take the above nano-selenium solution, add PEI and siRNA, stir magnetically for 1 h, centrifuge the reacted solution at 10,000 rpm for 10 minutes, was...

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Abstract

The invention discloses a nano-selenium loaded VP1 gene siRNA. The nano-selenium loaded VP1 gene siRNA is small in particle size, and can avoid an immune system to reach smallest capillaries, so thatresidence time is prolonged in a bloodstream; and the nano-selenium loaded VP1 gene siRNA has the characteristics of high target selectivity and long half-life. The genetic locus interfered by the siRNA is nucleotide of the 157th; and the siRNA sequence is: Sense 5'-ggcaucaucaaaugcuagutt-3', Antisense 5'-acuagcauuugaugaugcctt-3'. The invention also provides a preparation method and an applicationof the nano-selenium loaded VP1 gene siRNA.

Description

technical field [0001] The invention relates to VP1 gene siRNA loaded with nano-selenium, in particular to VP1 gene siRNA loaded with nano-selenium and its preparation method and application. Background technique [0002] Hand, foot and mouth disease is a common infectious disease caused by enteroviruses, mainly in infants and young children, with fever and rashes or herpes on the hands, feet, mouth and other parts as the main features. Most patients have mild symptoms, but a small number of patients may develop fatal complications such as myocarditis, pulmonary edema, acute flaccid paralysis, aseptic meningitis, encephalitis, and some severely ill children progress rapidly and easily cause death. Since the first large-scale outbreak of hand, foot and mouth disease in China in 2008, it has become popular every year to varying degrees, often causing kindergartens in various places to be forced to suspend classes and kindergartens, bringing huge negative impacts on society and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/713A61K9/19A61K47/02A61P31/14
CPCA61K9/19A61K47/02A61P31/14C12N15/1131C12N2310/14
Inventor 朱冰李英华林正方
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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