Establishment of method for judging similarity of pure species of Dendrobium denneanum, judging standard and judging method

Abstract

The invention relates to the establishment of a method for judging the similarity of pure species of Dendrobium denneanum, a judging standard and a judging method. The judging process of the method comprises the following steps: S1, collecting a sample of Dendrobium denneanum which is consistent with the gene sequencing conclusion, and measuring the diameter of the second internode stem of each sample; S2, performing normality test on the second internode stem diameter variable of the sample; S3, obtaining a standard interval: if the result obtained in the step S2 obeys the normal distribution, the 95% confidence interval of the mean value and the 95% confidence interval of the standard deviation are obtained according to a normal distribution totality calculation formula, and the confidence intervals can be used as standard ranges for identifying the purity of unknown samples. According to the collected data, the built judgment standard is that the 95% confidence interval of the meanvalue and the 95% confidence interval of the standard deviation are (4.0515, 4.4661) and (1.0178, 1.3137) respectively. The sample of the Dendrobium denneanum only needs to be compared with the intervals. The invention can identify the purity of Dendrobium denneanum varieties by the morphological characteristics, so that the value or existence of the artificially planted Dendrobium denneanum can be simply judged. The accuracy of identification is high, and important practical significance is achieved.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, in particular to the establishment of a method for judging the similarity of pure Dendrobium sheathes, judgment standards and judgment methods, that is, the method for establishing the correlation between the morphological characteristics of Dendrobium sheathes and the conclusion of gene sequencing and The identification method of the purity of the tested Dendrobium officinale species, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus in the family Orchidaceae, including Dendrobium sheath, Dendrobium fringe, Dendrobium officinale, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. It was l...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium sheathing.

However, the long-term artificial planting has also brought about false ones and shoddy ones, because ① artificial planting has changed the growth environment of wild Dendrobium; ② extensive application of various types of fertilizers, diseased , the application of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium sheathing; ③And because there are many varieties of Dendrobium, the hybridization between the varieties makes the traits of its close relatives cross; ④Others are uncontrollable Or unmeasurable factors, in summary, the medicinal ingredients of some artificially planted Dendrobium sheaths have changed, and even important medicinal ingredients have disappeared. Correspondingly, the medicinal ingredients of these medicinal ingredients have changed or disappeared The gene sequence is also substantially different from the original wild gene sequence

And once the medicinal value of these dendrobium sheaths weakens or disappears, and the field continues to use them without knowing it, the consequences will be very serious, and when people do not know it, the dendrobium sheaths will disappear from the medical field the consequences are more serious

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