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A method for promoting the proliferation and fermentation of Saccharomyces cerevisiae by using high-voltage pulsed electric field

A high-voltage pulsed electric field and Saccharomyces cerevisiae technology, applied in the field of bioengineering, can solve the problems of long fermentation cycle, low production efficiency, and high inoculation cost, and achieve the effects of shortened fermentation cycle, easy operation, and increased alcohol production

Active Publication Date: 2021-10-08
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional alcohol fermentation industry, there are common problems such as high inoculation cost, long fermentation cycle, and low production efficiency.

Method used

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  • A method for promoting the proliferation and fermentation of Saccharomyces cerevisiae by using high-voltage pulsed electric field

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) preparing seed medium and fermentation medium;

[0022] (2) Activation of strains and seed culture: Saccharomyces cerevisiae was streaked onto a solid seed medium, cultivated at 30°C for 24 hours, picked a single colony and inoculated in the seed medium, cultivated at 28°C until logarithmic growth phase, and obtained the need seed solution;

[0023] (3) High-voltage pulsed electric field stimulation and fermentation culture

[0024] Insert the seed solution in (2) into the fermentation medium with an inoculation amount of 5% by volume, connect the fermenter to a flow pump, place the fermenter in a high-voltage pulse electric field treatment chamber to perform high-voltage pulse electric field treatment, and carry out fermentation culture at the same time . The processing parameters of the high-voltage pulsed electric field are: the pulse frequency is 5Hz, the pulse width is 5μs, and the pulsed electric field intensity is 1kV cm -1 . The fermentation culture cond...

Embodiment 2

[0026] (1) preparing seed medium and fermentation medium;

[0027] (2) Activation of strains and seed culture: Saccharomyces cerevisiae was streaked onto a solid seed medium, cultivated at 30°C for 36 hours, picked a single colony and inoculated in the seed medium, cultivated at 32°C until the logarithmic growth phase, and obtained the need seed solution;

[0028] (3) High-voltage pulsed electric field stimulation and fermentation culture

[0029] The seed solution in (2) is inserted into the fermentation culture system according to the inoculation amount of 5% by volume fraction, and the fermentation system maintains a flow pump, and the fermenter is placed in a high-voltage pulse electric field treatment chamber for high-voltage pulse electric field treatment, and fermentation culture is carried out at the same time . The processing parameters of the high-voltage pulsed electric field are: the pulse width is 5 μs, the pulse frequency is 10 Hz, and the pulsed electric field...

Embodiment 3

[0031] (1) preparing seed medium and fermentation medium;

[0032] (2) Activation of strains and seed culture: Saccharomyces cerevisiae was streaked onto a solid seed medium, cultivated at 30°C for 3 hours, picked a single colony and inoculated in the seed medium, cultivated at 30°C until the logarithmic growth phase, and obtained the need seed solution;

[0033] (3) High-voltage pulsed electric field stimulation and fermentation culture

[0034] Insert the seed solution in (2) into the fermentation medium with an inoculation amount of 5% by volume, connect the fermenter to a flow pump, place the fermenter in a high-voltage pulse electric field treatment chamber to perform high-voltage pulse electric field treatment, and carry out fermentation culture at the same time . The processing parameters of the high-voltage pulsed electric field are: the pulse width is 5μs, the pulse frequency is 20Hz, and the pulsed electric field intensity is 6kV cm -1 . The fermentation culture ...

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Abstract

The invention discloses a method for promoting the proliferation and fermentation of Saccharomyces cerevisiae by using a high-voltage pulsed electric field. In the present invention, Saccharomyces cerevisiae is streaked to a solid seed medium for culture, and a single colony is picked and inoculated in the seed medium to be cultivated to the logarithmic growth phase to obtain the required seed liquid; the seed liquid is inserted into the fermentation culture system, and the fermentation culture system maintains The fermentation culture is carried out in a flowing state, and at the same time, the fermentation culture system is placed in a high-voltage pulse electric field treatment chamber for high-voltage pulse electric field treatment; the high-voltage pulse electric field treatment parameters are: pulse width 5μs, pulse frequency 5-20Hz, pulse electric field strength 1-20Hz 6kV·cm ‑1 ; The high-voltage pulsed electric field parameters used in the present invention can increase the activity of enzymes in yeast cells, and at the same time, the expression of related genes in the glycolysis pathway is improved. Under the stimulation of the high-voltage pulsed electric field parameters proposed by the invention, the proliferation speed and fermentation activity of Saccharomyces cerevisiae are strengthened, the fermentation period is shortened, and the alcohol output is increased.

Description

technical field [0001] The invention relates to promoting the proliferation and fermentation of Saccharomyces cerevisiae, relates to the field of bioengineering, in particular to a method for improving the growth, proliferation and fermentation of Saccharomyces cerevisiae by using a high-voltage pulse electric field. Background technique [0002] Saccharomyces cerevisiae is widely used in the fermentation industry, which can effectively utilize various sugar substances, and alcohol is the main product of its fermentation. In the traditional alcohol fermentation industry, there are common problems such as high inoculation cost, long fermentation cycle, and low production efficiency. High-voltage pulsed electric field is a physical processing technology that has emerged in recent years. It achieves the purpose of processing by applying an electric field under certain conditions. It has the advantages of high efficiency, low energy consumption, and continuous processing. It is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00C12N1/18C12R1/865
CPCC12N1/18C12N13/00
Inventor 应南娇范成凯章娟
Owner HANGZHOU DIANZI UNIV
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