Antioxidant activity detection method for traditional Chinese medicine preparation for activating blood to resolve stasis, and application of the method
A technology for antioxidant activity and traditional Chinese medicine preparation, which is applied in the measurement of color/spectral properties, etc., can solve the problem of no detection method for antioxidant activity, and achieve the effects of improving quality control standards, good reproducibility and fast analysis speed.
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Embodiment 1
[0034] This example provides a method for detecting the antioxidant activity of traditional Chinese medicine preparations for promoting blood circulation and removing blood stasis.
[0035] Take 0.5g of the content powder of Xuefu Zhuyu Capsules, accurately weigh it, place it in a 25mL volumetric flask, dilute to the mark with 50% v / v methanol, ultrasonicate for 10min, shake well, centrifuge at 14000rpm for 10min, and take the supernatant , and then diluted with 50% v / v methanol to 2.00mg / mL as the sample solution, set aside.
[0036] Take 25.00mg of DPPH reagent, weigh it accurately, place it in a 250mL brown volumetric flask, dissolve it with pure methanol, and set the volume to the mark, shake well to obtain the working solution of DPPH methanol.
[0037]Take 50 μL of the sample solution and add it to three parallel wells of a 96-well cell culture plate, and add 150 μL of DPPH methanol working solution as the solution to be tested.
[0038] Take 50 μL of the sample solutio...
Embodiment 2
[0042] This example provides a method for detecting the antioxidant activity of traditional Chinese medicine preparations for promoting blood circulation and removing blood stasis.
[0043] Take 0.5g of the content powder of Xuefu Zhuyu Capsules, accurately weigh it, place it in a 25mL volumetric flask, dilute to the mark with 75% v / v methanol, ultrasonicate for 10min, shake well, centrifuge at 14000rpm for 10min, and take the supernatant , and then diluted with 75% v / v methanol to 2.00mg / mL as the sample solution, set aside.
[0044] Take 25.00mg of DPPH reagent, weigh it accurately, place it in a 250mL brown volumetric flask, dissolve it with pure methanol, and set the volume to the mark, shake well to obtain the working solution of DPPH methanol.
[0045] Take 50 μL of the sample solution and add it to three parallel wells of a 96-well cell culture plate, and add 150 μL of DPPH methanol working solution as the solution to be tested.
[0046] Take 50 μL of the sample soluti...
Embodiment 3
[0050] This example provides a method for detecting the antioxidant activity of traditional Chinese medicine preparations for promoting blood circulation and removing blood stasis.
[0051] Take 0.5g of the content powder of Xuefu Zhuyu Capsules, accurately weigh it, place it in a 25mL volumetric flask, dilute to the mark with 25% v / v methanol, ultrasonicate for 10min, shake well, centrifuge at 14000rpm for 10min, and take the supernatant , and then diluted with 25% v / v methanol to 2.00mg / mL as the sample solution, set aside.
[0052] Take 25.00mg of DPPH reagent, weigh it accurately, place it in a 250mL brown volumetric flask, dissolve it with pure methanol, and set the volume to the mark, shake well to obtain the working solution of DPPH methanol.
[0053] Take 50 μL of the sample solution and add it to three parallel wells of a 96-well cell culture plate, and add 150 μL of DPPH methanol working solution as the solution to be tested.
[0054] Take 50 μL of the sample soluti...
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