Antioxidant activity detection method for traditional Chinese medicine preparation for activating blood to resolve stasis, and application of the method

A technology for antioxidant activity and traditional Chinese medicine preparation, which is applied in the measurement of color/spectral properties, etc., can solve the problem of no detection method for antioxidant activity, and achieve the effects of improving quality control standards, good reproducibility and fast analysis speed.

Pending Publication Date: 2018-11-30
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the problem that there is no antioxidant activity detection method in the quality standards of traditional Chinese medicine preparations for promoting blood circulation and removing bloo

Method used

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  • Antioxidant activity detection method for traditional Chinese medicine preparation for activating blood to resolve stasis, and application of the method
  • Antioxidant activity detection method for traditional Chinese medicine preparation for activating blood to resolve stasis, and application of the method
  • Antioxidant activity detection method for traditional Chinese medicine preparation for activating blood to resolve stasis, and application of the method

Examples

Experimental program
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Example Embodiment

[0033] Example 1

[0034] This embodiment provides a method for detecting the antioxidant activity of a traditional Chinese medicine preparation for promoting blood circulation and removing blood stasis.

[0035] Take 0.5g of the content of Xuefu Zhuyu Capsule powder, accurately weigh it, place it in a 25mL volumetric flask, dilute to the mark with 50% v / v methanol, sonicate for 10min, shake well, centrifuge at 14000rpm for 10min, take the supernatant , And then diluted with 50% v / v methanol to 2.00 mg / mL as a sample solution for use.

[0036] Take 25.00mg of DPPH reagent, accurately weigh it, place it in a 250mL brown volumetric flask, dissolve it with pure methanol, and dilute to the mark, shake it up, and then obtain the DPPH methanol working solution.

[0037] Take 50μL of the sample solution and add it to 3 parallel wells of a 96-well cell culture plate, and add 150μL of DPPH methanol working solution as the solution to be tested.

[0038] Take 50 μL of the sample solution and add...

Example Embodiment

[0041] Example 2

[0042] This embodiment provides a method for detecting the antioxidant activity of a traditional Chinese medicine preparation for promoting blood circulation and removing blood stasis.

[0043] Take 0.5g of the content of Xuefu Zhuyu Capsule powder, accurately weigh it, place it in a 25mL volumetric flask, dilute to volume with 75% v / v methanol, sonicate for 10min, shake well, centrifuge at 14000rpm for 10min, take the supernatant , And then diluted with 75% v / v methanol to 2.00mg / mL as a sample solution for use.

[0044] Take 25.00mg of DPPH reagent, accurately weigh it, place it in a 250mL brown volumetric flask, dissolve it with pure methanol, and dilute to the mark, shake it up, and then obtain the DPPH methanol working solution.

[0045] Take 50μL of the sample solution and add it to 3 parallel wells of a 96-well cell culture plate, and add 150μL of DPPH methanol working solution as the solution to be tested.

[0046] Take 50 μL of the sample solution and add it...

Example Embodiment

[0049] Example 3

[0050] This embodiment provides a method for detecting the antioxidant activity of a traditional Chinese medicine preparation for promoting blood circulation and removing blood stasis.

[0051] Take 0.5g of the content of Xuefu Zhuyu Capsule powder, accurately weigh it, place it in a 25mL volumetric flask, dilute to volume with 25% v / v methanol, sonicate for 10min, shake well, centrifuge at 14000rpm for 10min, take the supernatant , And then diluted with 25% v / v methanol to 2.00 mg / mL as a sample solution for use.

[0052] Take 25.00mg of DPPH reagent, accurately weigh it, place it in a 250mL brown volumetric flask, dissolve it with pure methanol, and dilute to the mark, shake it up, and then obtain the DPPH methanol working solution.

[0053] Take 50μL of the sample solution and add it to 3 parallel wells of a 96-well cell culture plate, and add 150μL of DPPH methanol working solution as the solution to be tested.

[0054] Take 50 μL of the sample solution and add i...

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Abstract

The invention discloses an antioxidant activity detection method for a traditional Chinese medicine preparation for activating blood to resolve stasis, which includes steps of: 1) ultrasonically extracting the traditional Chinese medicine preparation with a methanol solution to obtain a sample solution; 2) dissolving 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) in methanol to obtain a DPPH/methanol work solution; 3) mixing the sample solution, the DPPH/methanol work solution and methanol according to certain ratio to obtain a to-be-detected solution, a blank solution and a contrast solution,detecting the absorbancy of the solutions by means of a microplate reader, then calculating removal rate; 4) performing result correction with Vc being an internal standard substance, and expressing the antioxidant activity of the traditional Chinese medicine preparation by means of SR sample/SRVC. The invention also provides an application of the detection method for detecting the antioxidant activity of Xuefuzhuyu capsule.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis, and in particular relates to a method for detecting antioxidant activity of a traditional Chinese medicine preparation for promoting blood circulation and removing blood stasis and its application. Background technique [0002] Blood stasis syndrome includes blood stagnation in veins and viscera due to poor blood supply, or stagnation of blood from menstruation in the body, or syndrome caused by dirty blood, which can be seen in coronary heart disease, cerebrovascular disease, pulmonary heart disease, liver cirrhosis, etc. disease. Patients with blood stasis syndrome usually have at least one of abnormal platelet and coagulation function, hemodynamic changes, peripheral circulation disorder, inflammatory response, and connective tissue disease. The use of blood-activating and stasis-removing drugs, especially traditional Chinese medicine preparations for promoting b...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 柴欣张竞丁佳敏房士明杨静于卉娟张中群
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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