Method for preparing probiotic microcapsule by utilizing spray drying
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of probiotics and microcapsules, applied in the field of microorganisms, can solve the problem of high bacterial mortality, achieve the effect of ensuring the number of viable bacteria and increasing tolerance
Inactive Publication Date: 2018-12-07
广州富诺营养科技有限公司
View PDF4 Cites 5 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] Aiming at the deficiencies of the prior art, the present invention provides a method for preparing probiotic microcapsules by spray drying, which
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Example Embodiment
[0024] Example one:
[0025] A method for preparing probiotic microcapsules by spray drying includes the following steps:
[0026] 1. Preparation of culture medium: The culture medium is made up of the following parts by weight: 5 parts of yeast powder, 10 parts of peptone, 20 parts of glucose, 5 parts of sodium acetate trihydrate, 0.5 parts of magnesium sulfate heptahydrate, and 2 parts of potassium hydrogen phosphate. Parts, 2 parts of diammonium citrate, 10 parts of meat extract, 0.05 parts of manganese sulfate tetrahydrate, Tween-801 parts, 1000 parts of purified water; mix yeast powder, peptone, glucose, sodium acetate trihydrate, magnesium sulfate heptahydrate , Dipotassium hydrogen phosphate, diammonium citrate, meat extract 10, manganese sulfate tetrahydrate, Tween-80 in accordance with the ratio, pour into purified water and heat to dissolve, dissolve, disassemble, and place in a sterilization pot at 121℃ Sterilize for 20 minutes under the conditions, and set aside;
[002...
Example Embodiment
[0038] Embodiment two:
[0039] The basic steps of this embodiment are the same as the first embodiment, the difference is: in step 3, place the culture in a 37°C incubator. After 12 hours of incubation, put the petri dish into a 70°C constant temperature water bath and bath for 5 minutes.
Example Embodiment
[0040] Example three:
[0041] The basic steps of this embodiment are the same as those of the first embodiment. The difference is: in step 3, the culture is placed in a 37°C incubator. After 13 hours of cultivation, the petri dish is placed in a 80°C constant temperature water bath and the water bath is bathed for 6 minutes.
[0042] The comparison table of the embodiment of the present invention and the existing preparation method under the same experimental conditions:
[0043]
[0044] It can be seen from the above table that, compared with the existing preparation method, the product of the present invention can effectively increase the number of viable bacterial powder.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for preparing a probiotic microcapsule by utilizing spray drying. The method comprises the following steps:1, preparation of a culture medium; 2, activation of thallus; 3, proliferation and heat shock of the thallus: inoculating the activated thallus according to 1 percent of inoculation quantity, putting the activated thallus in a 37 DEG C-constant temperature boxand performing culturing; and putting a culture dish into a constant-temperature water bath pot of 60 DEG C to 80 DEG C after culturing is carried out for 11 hours to 13 hours, and then performing water bath for 4 minutes to 6 minutes; 4, concentration of the thallus: pouring culture liquid after heat shock into a sterilizing tube, putting the sterilizing tube into a centrifugal machine, settingrotation speed of the centrifugal machine as 6000r/min, and ensuring that the temperature is 4 DEG C and the centrifugation time is 10 minutes; 5, preparation of mixed suspension; 6, spray drying; and7, performing packaging. The invention relates to the technical field of microorganisms. By adopting the method for preparing the probiotic microcapsule by utilizing the spray drying, the problem ofa high death ratio of the thallus caused by a higher temperature in a drying machine is solved, and the viable count of probiotic powder is ensured.
Description
technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for preparing probiotic microcapsules by spray drying. Background technique [0002] Probiotics, also known as prebiotics, probiotics, active bacteria, and microecological preparations, refer to those live bacterial products that maintain the balance of human flora and have beneficial effects on human health or contain bacterial components and metabolites. Dead bacteria products. The types of probiotics currently used in humans mainly include Lactobacillus, Bifidobacterium, Enterococcus, Lactococcus, Bacillus subtilis, Escherichia coli, Bacillus cereus, Bacillus licheniformis, Streptococcus thermophilus, Clostridium butyricum and Yeast, etc., but traditionally people use mainly two genera of Bifidobacterium and Lactobacillus. However, the most critical problem of probiotic preparations is that the number of viable bacteria in the product decreases rapidly. Accor...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more